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Vol. 26 No. 4 (2014): Vol 26 Issue 4

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Enhanced Production of Rhamnolipids by Pseudomonas aeruginosa JQ927360 Using Response Surface Methodology

https://doi.org/10.14233/ajchem.2014.15851
A. Jamal
Department of Chemistry, Government College University, Lahore, Pakistan
M.Z. Qureshi
Department of Chemistry, Government College University, Lahore, Pakistan
N. Ali
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
M.I. Ali
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan
A. Hameed
Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan

Corresponding Author(s) : A. Jamal

asifjamall@yahoo.com

Asian Journal of Chemistry, Vol. 26 No. 4 (2014): Vol 26 Issue 4

Abstract

Rhamnolipids are fascinating microbial surfactants having great industrial importance. Present study was focused to investigate the role of some critical fermentation parameters for the production of rhamnolipids from P. aeruginosa JQ927360. The process for the production of rhamnolipids was optimized using central composite design and response surface methodology. The factors subjected for studies were pH, temperature, shaking speed and inoculum size. The maximum rhamnolipids yield of 4.44 g/L was achieved at optimum level of process variable i.e., pH 7, 33 °C, 155 rpm and 2.8 % inoculum size. The results of the present investigation suggested that application of mathematical modeling remained very effective to determine optimum reaction conditions and improved the yield of given bioprocess.

Keywords

Rhamnolipids P. aeruginosa Response surface methodology Biosurfactants Optimization
Jamal, A., Qureshi, M., Ali, N., Ali, M., & Hameed, A. (2014). Enhanced Production of Rhamnolipids by Pseudomonas aeruginosa JQ927360 Using Response Surface Methodology. Asian Journal of Chemistry, 26(4), 1044–1048. https://doi.org/10.14233/ajchem.2014.15851
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