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Estimation of Antioxidant Power in Various Extracts of Euphorbia helioscopia L. with Five Different in vitro Antioxidant Models
Corresponding Author(s) : Bashir Ahmad
Asian Journal of Chemistry,
Vol. 26 No. 4 (2014): Vol 26 Issue 4
Abstract
Reactive oxygen species and reactive nitrogen species are generated within living body as a result of redox reactions and these may damage biomolecules i.e. lipids, proteins and DNA that cause number of pathological conditions. Plants are rich source of antioxidants that can combat oxidative stress-induced diseases. Various in vitro methods have been designed to assess antioxidant activity of medicinal plants. The aim of current study was to evaluate antioxidant activity of Euphorbia helioscopia extracts and latex employing five in vitro antioxidant assays (DPPH, TAC, FRAP, FTC and BCL). The data showed promising antioxidant activity in plant latex (highest) and L.MT (second highest) in all the methods. The other extracts did also show appreciable antioxidant activity but significantly lower than latex and L.MT. Significant positive Pearson correlation (at 0.01 level, two tailed) was also found among all the methods. It is concluded that the plants rich in antioxidants could be helpful in combating diseases.
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- A. Arora, R.K. Sairam and G.S. Srivastava, Curr. Sci., 82, 1227 (2002).
- L. Bergendi, L. Benes, Z. Durackova and M. Ferencik, Life Sci., 65, 1865 (1999); doi:10.1016/S0024-3205(99)00439-7.
- Y.-Z. Fang, S. Yang and G. Wu, Nutrition, 18, 872 (2002); doi:10.1016/S0899-9007(02)00916-4.
- A. Saraswathy, D. Ramasamy and D.S. Nandini, Indian Drugs., 45, 631 (2008).
- A. Shirwaikar, K.S. Prabhu and I.S.R. Punitha, Indian J. Exp. Biol., 44, 993 (2006).
- I.M. Lee, N.R. Cook, J.E. Manson, J.E. Buring and C.H. Hennekens, J. Natl. Cancer Inst., 91, 2102 (1999); doi:10.1093/jnci/91.24.2102.
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- T. Sun and C.T. Ho, Food Chem., 90, 743 (2005); doi:10.1016/j.foodchem.2004.04.035.
- M.J. Engelhart, M.I. Geerlings and A. Ruitenberg, JAMA, 287, 3223 (2002); doi:10.1001/jama.287.24.3223.
- M.C. Sabu and R. Kuttan, J. Ethnopharmacol., 81, 155 (2002); doi:10.1016/S0378-8741(02)00034-X.
- E.A. Ostrakhovitch and I.B. Afanas’ev, Biochem. Pharmacol., 62, 743 (2001); doi:10.1016/S0006-2952(01)00707-9.
- D. Harman, Drugs Aging, 3, 60 (1993); doi:10.2165/00002512-199303010-00006.
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- D. Huang, B. Ou and R.L. Prior, J. Agric. Food Chem., 53, 1841 (2005); doi:10.1021/jf030723c.
- H.E. Miller, J. Am. Oil Chem. Soc., 48, 91 (1971); doi:10.1007/BF02635693.
- E.N. Frankel, Dundee, 10, 23 (1998).
- G.K. Jayaprakasha and R.L. Jaganmohan, Z. Naturforsch, 55c, 1018 (2000).
- W.B. Williams, M.E. Cuvelier and C. Berset, Lebensmittel-Wissen.–Technol./Food Sci. Technol., 28, 25 (1995).
- I.E.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996); doi:10.1006/abio.1996.0292.
- P. Prieto, M. Pineda and M. Aguilar, Anal. Biochem., 269, 337 (1999); doi:10.1006/abio.1999.4019.
- H. Mistuda, K. Yuasumoto and K. Iwami, EiyoTo Shokuryo, 19, 210 (1996).
- C. Wong, H. Li, K. Cheng and F. Chen, Food Chem., 97, 705 (2006); doi:10.1016/j.foodchem.2005.05.049.
- M. Uzair, B.A. Loothar and B.A. Choudhary, Pak. J. Pharm. Sci., 22, 18 (2009).
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- P.X. Nunefs, S.F. Silva, R.J. Guedes and S. Almeida, Biological oxidations and antioxidant activity of natural products. Phytochemicals as nutraceuticals-Global Approaches to Their Role in Nutrition and Health (2012).
- G. Cao, E. Sofic and R.L. Prior, Free Radic. Biol. Med., 22, 749 (1997); doi:10.1016/S0891-5849(96)00351-6.
- S. Uzma, H. Khalid, A. Mobashir, I.B. Nadeem, M. Arif and A. Bashir, Pak. J. Pharm. Sci. (2014) (in Press).
- G. Di Carlo, N. Mascolo, A.A. Izzo and F. Capasso, Life Sci., 65, 337 (1999); doi:10.1016/S0024-3205(99)00120-4.
- P. Montoro, A. Braca, C. Pizza and N. Detommasi, Food Chem., 92, 349 (2005); doi:10.1016/j.foodchem.2004.07.028.
References
A. Arora, R.K. Sairam and G.S. Srivastava, Curr. Sci., 82, 1227 (2002).
L. Bergendi, L. Benes, Z. Durackova and M. Ferencik, Life Sci., 65, 1865 (1999); doi:10.1016/S0024-3205(99)00439-7.
Y.-Z. Fang, S. Yang and G. Wu, Nutrition, 18, 872 (2002); doi:10.1016/S0899-9007(02)00916-4.
A. Saraswathy, D. Ramasamy and D.S. Nandini, Indian Drugs., 45, 631 (2008).
A. Shirwaikar, K.S. Prabhu and I.S.R. Punitha, Indian J. Exp. Biol., 44, 993 (2006).
I.M. Lee, N.R. Cook, J.E. Manson, J.E. Buring and C.H. Hennekens, J. Natl. Cancer Inst., 91, 2102 (1999); doi:10.1093/jnci/91.24.2102.
B. Wang, W. Zhang, X. Duan and X. Li, Food Chem., 113, 1101 (2009); doi:10.1016/j.foodchem.2008.08.078.
T. Sun and C.T. Ho, Food Chem., 90, 743 (2005); doi:10.1016/j.foodchem.2004.04.035.
M.J. Engelhart, M.I. Geerlings and A. Ruitenberg, JAMA, 287, 3223 (2002); doi:10.1001/jama.287.24.3223.
M.C. Sabu and R. Kuttan, J. Ethnopharmacol., 81, 155 (2002); doi:10.1016/S0378-8741(02)00034-X.
E.A. Ostrakhovitch and I.B. Afanas’ev, Biochem. Pharmacol., 62, 743 (2001); doi:10.1016/S0006-2952(01)00707-9.
D. Harman, Drugs Aging, 3, 60 (1993); doi:10.2165/00002512-199303010-00006.
W. Zheng and S.Y. Wang, J. Agric. Food Chem., 49, 5165 (2001); doi:10.1021/jf010697n.
Y.Z. Cai, M. Sun and H. Corke, J. Agric. Food Chem., 51, 2288 (2003); doi:10.1021/jf030045u.
D. Huang, B. Ou and R.L. Prior, J. Agric. Food Chem., 53, 1841 (2005); doi:10.1021/jf030723c.
H.E. Miller, J. Am. Oil Chem. Soc., 48, 91 (1971); doi:10.1007/BF02635693.
E.N. Frankel, Dundee, 10, 23 (1998).
G.K. Jayaprakasha and R.L. Jaganmohan, Z. Naturforsch, 55c, 1018 (2000).
W.B. Williams, M.E. Cuvelier and C. Berset, Lebensmittel-Wissen.–Technol./Food Sci. Technol., 28, 25 (1995).
I.E.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996); doi:10.1006/abio.1996.0292.
P. Prieto, M. Pineda and M. Aguilar, Anal. Biochem., 269, 337 (1999); doi:10.1006/abio.1999.4019.
H. Mistuda, K. Yuasumoto and K. Iwami, EiyoTo Shokuryo, 19, 210 (1996).
C. Wong, H. Li, K. Cheng and F. Chen, Food Chem., 97, 705 (2006); doi:10.1016/j.foodchem.2005.05.049.
M. Uzair, B.A. Loothar and B.A. Choudhary, Pak. J. Pharm. Sci., 22, 18 (2009).
G. Perry, A.K. Raina, A. Nunomura, T. Wataya, L.M. Sayre and M.A. Smith, Free Radic. Biol. Med., 28, 831 (2000); doi:10.1016/S0891-5849(00)00158-1.
P.X. Nunefs, S.F. Silva, R.J. Guedes and S. Almeida, Biological oxidations and antioxidant activity of natural products. Phytochemicals as nutraceuticals-Global Approaches to Their Role in Nutrition and Health (2012).
G. Cao, E. Sofic and R.L. Prior, Free Radic. Biol. Med., 22, 749 (1997); doi:10.1016/S0891-5849(96)00351-6.
S. Uzma, H. Khalid, A. Mobashir, I.B. Nadeem, M. Arif and A. Bashir, Pak. J. Pharm. Sci. (2014) (in Press).
G. Di Carlo, N. Mascolo, A.A. Izzo and F. Capasso, Life Sci., 65, 337 (1999); doi:10.1016/S0024-3205(99)00120-4.
P. Montoro, A. Braca, C. Pizza and N. Detommasi, Food Chem., 92, 349 (2005); doi:10.1016/j.foodchem.2004.07.028.