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Vol. 26 No. 4 (2014): Vol 26 Issue 4

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Optimization Process of Total Phenol from Suaeda glauca Bge Leaf by Response Surface Methodology and Free Radical Scavenging Activity of Extract

https://doi.org/10.14233/ajchem.2014.16329
X.H. Wang
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Department of Chemistry, Yancheng Teachers University, Xiwang Road, Yancheng 224051, Jiangsu Province, P.R. China Institute of Applied Chemistry & Environmental Engineering, Department of Chemistry, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, P.R. China
J.T. Dai
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Department of Chemistry, Yancheng Teachers University, Xiwang Road, Yancheng 224051, Jiangsu Province, P.R. China Institute of Applied Chemistry & Environmental Engineering, Department of Chemistry, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, P.R. China
J.P. Wang
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Department of Chemistry, Yancheng Teachers University, Xiwang Road, Yancheng 224051, Jiangsu Province, P.R. China Institute of Applied Chemistry & Environmental Engineering, Department of Chemistry, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, P.R. China
Y.L. Gu
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Department of Chemistry, Yancheng Teachers University, Xiwang Road, Yancheng 224051, Jiangsu Province, P.R. China Institute of Applied Chemistry & Environmental Engineering, Department of Chemistry, Yancheng Teachers University, Yancheng 224051, Jiangsu Province, P.R. China

Corresponding Author(s) : X.H. Wang

wangxhong1227@163.com

Asian Journal of Chemistry, Vol. 26 No. 4 (2014): Vol 26 Issue 4

Abstract

Suaeda glauca Bge leaf extract was evaluated for total phenolic content and antioxidant capacity. The extraction process of total phenolics based microwave-assisted extraction was optimized by single-factor experiment and response surface methodology, which parameters included the solid-liquid ratio, extraction time and extraction cycle. Under the optimized experimental conditions, the best results were obtained that in highest extraction total phenolic content was 2624.29 ug g-1 with microwave time for 68.34 min, liquid-solid ratio for 62.33 mL g-1 and three time extraction. The proposed microwave-assisted extraction was an efficient extraction procedure with shorter extraction time and remarkable higher efficiencies. The assay of antioxidant capacity contained potential free radical scavenging activities for 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), hydroxyl radical (OH), superoxide radicals (O2–) and hydrogen peroxide (H2O2), which compared with standards VC/BHT. The present study indicated that Suaeda glauca Bge leaf possess a strong anti-radical activity and may be considering to make efforts in the oxidation resistance of application.

Keywords

Microwave-assisted extraction Antiradical Response surface methodology Total phenols
Wang, X., Dai, J., Wang, J., & Gu, Y. (2014). Optimization Process of Total Phenol from Suaeda glauca Bge Leaf by Response Surface Methodology and Free Radical Scavenging Activity of Extract. Asian Journal of Chemistry, 26(4), 1215–1220. https://doi.org/10.14233/ajchem.2014.16329
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