Copyright (c) 2014 AJC
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Study on Polymer Formation and Film-forming Properties Under Catalysis of Transglutaminase Between Casein and Keratin
Corresponding Author(s) : Hong-Ru Wang
Asian Journal of Chemistry,
Vol. 26 No. 4 (2014): Vol 26 Issue 4
Abstract
Different mass ratio of casein and keratin were used to produce heteropolymer under the treatment of transglutaminase. The molecular weight of the polymer was investigated by SDS-PAGE. Films mechanical and water vapor barrier properties of the blended film were also studied. Film morphology was observed by using scanning electron microscopy. The crosslinking between casein and keratin proved by electrophoresis and the molecular weight of the new polymer was presumed about 50-70 kDa. The casein/keratin blended films showed an increase in elongation and water vapor permeability with transglutaminase treatment. The synergistic effect can be seen from the elongation value of the transglutamiase treatment casein/keratin blended filmed at the mass ratio of 4:1. The value of the tensile strength of the blended films decreased significantly with the increasing content of keratin.
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- C. Partschefeld, U. Schwarzenbolz, S. Richter and T. Henle, Biotechnol. J., 2, 456 (2007); doi:10.1002/biot.200600232.
- M.M. Taylor, C. Liu, W.N. Marmer and E.M. Brown, J. Am. Leather Chemists Assoc., 98, 435 (2003).
- N. Nio, M. Motoki and K. Takinami, Agric. Biol. Chem., 49, 2283 (1985); doi:10.1271/bbb1961.49.2283.
- E.E. Babiker, Food Chem., 70, 139 (2000); doi:10.1016/S0308-8146(99)00231-9.
- L.T. Lim, Y. Mine and M.A. Tung, J. Agric. Food Chem., 46, 4022 (1998); doi:10.1021/jf980567n.
- J.M. Cardamone and J.G. Phillips, Text. Res. J., 77, 277 (2007); doi:10.1177/0040517507078788.
- D.R. Kahn and I. Cohen, Biochim. Biophys. Acta, 668, 490 (1981); doi:10.1016/0005-2795(81)90184-7.
- M.C. Alexandre, Y. Popineau, G. Viroben, M. Chiarello, A. Lelion and J. Gueguen, Agric. Food Chem., 41, 2208 (1993); doi:10.1021/jf00035a073.
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- A. Basman, H. Köksel and P.K.W. Ng, Eur. Food Res. Technol., 215, 419 (2002); doi:10.1007/s00217-002-0573-3.
- M. Yildirim and N.S. Hettiarachchy, J. Food Sci., 63, 248 (1998); doi:10.1111/j.1365-2621.1998.tb15719.x.
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- J.C. Ramirez-Suarez and Y.L. Xiong, Meat Science, 65, 899 (2003); doi: 10.1016/S0309-1740(02)00297-8.
- J.H. Oh, B. Wang, P.D. Field and H.A. Aglan, J. Food Sci. Technol., 39, 287 (2004); doi:10.1111/j.1365-2621.2004.00783.x.
- A. Bonet, W. Blaszczak and C.M. Rosell, Cereal Chem., 83, 655 (2006); doi:10.1094/CC-83-0655.
- E. Hernàndez-Balada, M.M. Taylor, J.G. Phillips, W.N. Marmer and E.M. Brown, Bioresour. Technol., 100, 3638 (2009); doi:10.1016/j.biortech.2009.02.039.
- L. Kurth, Food Technol. Austr., 35, 420 (1983).
- H. Chambi and C. Grosso, Food Res. Int., 39, 458 (2006); doi:10.1016/j.foodres.2005.09.009.
- L. Cui, X. Fan, P. Wang, Q. Wang and G. Fu, Eng. Life Sci., 11, 201 (2011); doi:10.1002/elsc.201000110.
- C. Li, W. Qiang, W. Ping and H. Qingsong, J. Appl. Polymer, 4, 2053 (2011).
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- H. Chen, J. Dairy Sci., 78, 2563 (1995); doi:10.3168/jds.S0022-0302(95)76885-0.
- S. Reichl, M. Borrelli and G. Geerling, Biomaterials, 32, 3375 (2011); doi:10.1016/j.biomaterials.2011.01.052.
- T. Tanabe, N. Okitsu, A. Tachibana and K. Yamauchi, Biomaterials, 23, 817 (2002); doi:10.1016/S0142-9612(01)00187-9.
- N.K. Howell, Biopolymer Mixtures, University Press, Nottingham, pp. 329-347 (1995).
References
C. Partschefeld, U. Schwarzenbolz, S. Richter and T. Henle, Biotechnol. J., 2, 456 (2007); doi:10.1002/biot.200600232.
M.M. Taylor, C. Liu, W.N. Marmer and E.M. Brown, J. Am. Leather Chemists Assoc., 98, 435 (2003).
N. Nio, M. Motoki and K. Takinami, Agric. Biol. Chem., 49, 2283 (1985); doi:10.1271/bbb1961.49.2283.
E.E. Babiker, Food Chem., 70, 139 (2000); doi:10.1016/S0308-8146(99)00231-9.
L.T. Lim, Y. Mine and M.A. Tung, J. Agric. Food Chem., 46, 4022 (1998); doi:10.1021/jf980567n.
J.M. Cardamone and J.G. Phillips, Text. Res. J., 77, 277 (2007); doi:10.1177/0040517507078788.
D.R. Kahn and I. Cohen, Biochim. Biophys. Acta, 668, 490 (1981); doi:10.1016/0005-2795(81)90184-7.
M.C. Alexandre, Y. Popineau, G. Viroben, M. Chiarello, A. Lelion and J. Gueguen, Agric. Food Chem., 41, 2208 (1993); doi:10.1021/jf00035a073.
C. Larré, S. Denery-Papini, Y. Popineau, G. Deshayes, C. Desserme and J. Lefebvre, Cereal Chem., 77, 121 (2000); doi:10.1094/CCHEM.2000.77.2.121.
A. Basman, H. Köksel and P.K.W. Ng, J. Food Sci., 67, 2654 (2002); doi:10.1111/j.1365-2621.2002.tb08794.x.
A. Basman, H. Köksel and P.K.W. Ng, Eur. Food Res. Technol., 215, 419 (2002); doi:10.1007/s00217-002-0573-3.
M. Yildirim and N.S. Hettiarachchy, J. Food Sci., 63, 248 (1998); doi:10.1111/j.1365-2621.1998.tb15719.x.
M. Yildirim and N.S. Hettiarachchy, J. Food Sci., 62, 270 (1997); doi:10.1111/j.1365-2621.1997.tb03983.x.
K. Takahashi, Y. Nakata, K. Someya and M. Hattori, Biosci. Biotechnol. Biochem., 63, 2144 (1999); doi:10.1271/bbb.63.2144.
J.C. Ramirez-Suarez and Y.L. Xiong, Meat Science, 65, 899 (2003); doi: 10.1016/S0309-1740(02)00297-8.
J.H. Oh, B. Wang, P.D. Field and H.A. Aglan, J. Food Sci. Technol., 39, 287 (2004); doi:10.1111/j.1365-2621.2004.00783.x.
A. Bonet, W. Blaszczak and C.M. Rosell, Cereal Chem., 83, 655 (2006); doi:10.1094/CC-83-0655.
E. Hernàndez-Balada, M.M. Taylor, J.G. Phillips, W.N. Marmer and E.M. Brown, Bioresour. Technol., 100, 3638 (2009); doi:10.1016/j.biortech.2009.02.039.
L. Kurth, Food Technol. Austr., 35, 420 (1983).
H. Chambi and C. Grosso, Food Res. Int., 39, 458 (2006); doi:10.1016/j.foodres.2005.09.009.
L. Cui, X. Fan, P. Wang, Q. Wang and G. Fu, Eng. Life Sci., 11, 201 (2011); doi:10.1002/elsc.201000110.
C. Li, W. Qiang, W. Ping and H. Qingsong, J. Appl. Polymer, 4, 2053 (2011).
J.M. Cardamone, J. Mol. Struct., 969, 97 (2010); doi:10.1016/j.molstruc.2010.01.048.
H. Chen, R. Banerjee and E.J. Limoge, in eds.: I.A. Taub and R. Bell, Testing Mechanical Strengths of Edible Films; in Proceedings of Food Preservation 2000, Science and Technology Corporation, Hampton, VA, pp. 863-875 (1993).
H. Chen, J. Dairy Sci., 78, 2563 (1995); doi:10.3168/jds.S0022-0302(95)76885-0.
S. Reichl, M. Borrelli and G. Geerling, Biomaterials, 32, 3375 (2011); doi:10.1016/j.biomaterials.2011.01.052.
T. Tanabe, N. Okitsu, A. Tachibana and K. Yamauchi, Biomaterials, 23, 817 (2002); doi:10.1016/S0142-9612(01)00187-9.
N.K. Howell, Biopolymer Mixtures, University Press, Nottingham, pp. 329-347 (1995).