Copyright (c) 2014 AJC
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Simultaneous Assay of Three Diterpenoids with One Marker in Semen Euphorbiae pulveratum
Corresponding Author(s) : Zhenling Zhang
Asian Journal of Chemistry,
Vol. 26 No. 4 (2014): Vol 26 Issue 4
Abstract
Euphorbia is a widely used traditional Chinese medicine (TCM) from the dry mature seed of Euphorbia lathyris L. which can enhance diuresis. Diterpenoids such as euphorbia factor L1, euphorbia factor L2 and euphorbia factor L3, are the main active ingredients. In present work, the researchers attempted to establish a quantitative method for the simultaneous assay of three diterpenoids with one marker in semen Euphorbiae pulveratum and then validated its feasibility and durability in controlling the quality of semen Euphorbiae pulveratum. High-performance liquid chromatography method was used for the experiment. The retention value relative to the chromatographic peak of the component to be measured was determined and then the relative correction factor and the relative retention value were computed. Finally, the relative error was established to be less than 5 % by comparing the quantitative results of quantitative analysis of multi-components by single marker (QAMS) with those of the external standard method. Overall, the usage of QAMS is feasible for the simultaneous determination of euphorbia factor L1, euphorbia factor L2 and euphorbia factor L3, as no significant differences were observed between the measured values and the calculated values.
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- Y.S. Wang, G.W. Song, H.W. Zhang, R.H. Wu and Z.L. Zhang, Asian J. Chem., 25, 10011 (2013); doi:10.14233/ajchem.2013.16001.
- Y.X. Li, Z.X. Sun, Y. Li and J.-X. Lu, China J. Tradit. Chinese Med. Pharmacy, 23, 614 (2008).
- S.-H. Li and C.J.-P.H. Ying, Asian J. Chem., 25, 2331 (2013).
- N. Dubey, A. Jain, J. Sardiya and D.K. Jain, Asian J. Chem., 24, 4644 (2012).
- Y.-R. Gao, Z.-H. Zhao and M.-J. Liu, Asian J. Chem., 24, 792 (2012).
- Y.X. Li, M. Yuan, Y.Y. Chen, L.J. Hou, X. Shao and Q.Q. Ci, Chinese Tradit. Patent Medicine, 32, 440 (2010).
- F.L. Zheng, H.H. Ning, S.C. Ma and Y.H. Luo, Chin. Tradit. Herbal Drugs, 40, 1656 (2009).
- X. Meng, Y.N. Wang, H.F. Zhuang, Y. Yuan, K.S. Bi and C. Xiao-Hui, Chinese J. Experimental Tradit. Med. Formulae, 18, 91 (2012).
- Z.-M. Wang, H.-M. Gao, X.-T. Fu and W.-H. Wang, Chinese J. Chin. Materia Medica, 31, 1925 (2006).
- J.J. Zhu, Z.M. Wang, Q.-W Zhang, J.-W. Niu, F. Li, Chinese J. Chin. Materia Medica, 34, 3229 (2009).
- J.J. Zhu, Z.M. Wang, Y.H. Kuang, Q.W. Zhang, Q.P. Gao and N. Ma, Acta Pharmacol. Sin., 43, 1211 (2008).
- A.P. Li, X. Yang, Y.H. Ding and J.B. Zhao, Chinese J. Pharm. Anal., 32, 1534 (2012).
- C. Li, H.X. Yang and Y.C. Xiao, Chinese J. Pharm. Anal., 31, 1658 (2011).
- Y.Z. Fu, Y.L. Feng, L. Cao and Y. Wang, Chinese J. Pharm. Anal., 32, 775 (2012).
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- W.G. Song, F.J. Sun, M. Zhang, H.J. Yu and Y.X. Li, Pharmacol. Clinics Chinese Mater. Medica, 26, 40 (2010).
References
Y.S. Wang, G.W. Song, H.W. Zhang, R.H. Wu and Z.L. Zhang, Asian J. Chem., 25, 10011 (2013); doi:10.14233/ajchem.2013.16001.
Y.X. Li, Z.X. Sun, Y. Li and J.-X. Lu, China J. Tradit. Chinese Med. Pharmacy, 23, 614 (2008).
S.-H. Li and C.J.-P.H. Ying, Asian J. Chem., 25, 2331 (2013).
N. Dubey, A. Jain, J. Sardiya and D.K. Jain, Asian J. Chem., 24, 4644 (2012).
Y.-R. Gao, Z.-H. Zhao and M.-J. Liu, Asian J. Chem., 24, 792 (2012).
Y.X. Li, M. Yuan, Y.Y. Chen, L.J. Hou, X. Shao and Q.Q. Ci, Chinese Tradit. Patent Medicine, 32, 440 (2010).
F.L. Zheng, H.H. Ning, S.C. Ma and Y.H. Luo, Chin. Tradit. Herbal Drugs, 40, 1656 (2009).
X. Meng, Y.N. Wang, H.F. Zhuang, Y. Yuan, K.S. Bi and C. Xiao-Hui, Chinese J. Experimental Tradit. Med. Formulae, 18, 91 (2012).
Z.-M. Wang, H.-M. Gao, X.-T. Fu and W.-H. Wang, Chinese J. Chin. Materia Medica, 31, 1925 (2006).
J.J. Zhu, Z.M. Wang, Q.-W Zhang, J.-W. Niu, F. Li, Chinese J. Chin. Materia Medica, 34, 3229 (2009).
J.J. Zhu, Z.M. Wang, Y.H. Kuang, Q.W. Zhang, Q.P. Gao and N. Ma, Acta Pharmacol. Sin., 43, 1211 (2008).
A.P. Li, X. Yang, Y.H. Ding and J.B. Zhao, Chinese J. Pharm. Anal., 32, 1534 (2012).
C. Li, H.X. Yang and Y.C. Xiao, Chinese J. Pharm. Anal., 31, 1658 (2011).
Y.Z. Fu, Y.L. Feng, L. Cao and Y. Wang, Chinese J. Pharm. Anal., 32, 775 (2012).
Z.-Y. Hu, W. Hua, L. Zhang, L.-B. Deng, X.-F. Wang, G.-H. Liu, W.-J. Hao and H.-Z. Wang, PLoS ONE, 8, e62099 (2013); doi:10.1371/journal.pone.0062099.
W.L. Wei, M.Y. Jin, C. Ma, S.-S Feng, China Oils Fats, 32, 70 (2007).
F.J. Sun, W.G. Song and Y.X. Li, J. Trad. Chin. Med., 38, 1450 (2011).
F.-J. Sun, W.-G. Song and Y.-X. Li, Chin. J. Pharma Covigilance, 8, 20 (2011).
W.G. Song, F.J. Sun, M. Zhang, H.J. Yu and Y.X. Li, Pharmacol. Clinics Chinese Mater. Medica, 26, 40 (2010).