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Vol. 26 No. 2 (2014): Vol 26 Issue 2

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Antimicrobial Activity of Extract and Fractions of Different Parts and GC-MS Profiling of Essential Oil of Cichorium intybus Extracted by Super Critical Fluid Extraction

https://doi.org/10.14233/ajchem.2014.15667
Muhammad Afzal1
1Department of Chemistry & Biochemistry, University of Agriculture Faisalabad-38040, Pakistan 2Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan 3Department of Chemistry, Shah Abdul Latif University, Khairpur, Sindh, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com
Muhammad Shahid1
1Department of Chemistry & Biochemistry, University of Agriculture Faisalabad-38040, Pakistan 2Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan 3Department of Chemistry, Shah Abdul Latif University, Khairpur, Sindh, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com
Zahid Mehmood1
1Department of Chemistry & Biochemistry, University of Agriculture Faisalabad-38040, Pakistan 2Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan 3Department of Chemistry, Shah Abdul Latif University, Khairpur, Sindh, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com
Shazia Anwer Bukhari2
1Department of Chemistry & Biochemistry, University of Agriculture Faisalabad-38040, Pakistan 2Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan 3Department of Chemistry, Shah Abdul Latif University, Khairpur, Sindh, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com
Mir Munsif Ali Talpur3
1Department of Chemistry & Biochemistry, University of Agriculture Faisalabad-38040, Pakistan 2Department of Applied Chemistry and Biochemistry, Government College University, Faisalabad, Pakistan 3Department of Chemistry, Shah Abdul Latif University, Khairpur, Sindh, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com

Asian Journal of Chemistry, Vol. 26 No. 2 (2014): Vol 26 Issue 2

Abstract

Present study was conducted to appraise the antimicrobial potential of organic extracts of different parts of plant. Cichorium intybus widely known for their antimicrobial potential was used in the present investigation. Dry root, stem and leaves of medicinal plant was grounded to fine powder and extracted in methanol for 20 days. After this, methanol was allowed to evaporate and resulting residue was dissolved in different sequential solvents viz., hexane, ethyl acetate, n-butanol and water. Fraction of different organic solvents were used for antimicrobial activity. Essential oil of whole plant was extracted using supercritical fluid extraction method. Chemical profiling of essential oil was determined using GC-MS analysis. It is evident from the results of GC-MS analysis that essential oil of medicinal plant contains biological active organic compounds. Essential oil was also used against different bacterial and fungal strains that exhibited conspicuous antimicrobial activity. selected microorganisms Aspergillus niger, Fusarium solnai, Staphylococcus aureus and Escherichia coli were tested with activity (20 ± 1, 20 ± 0.6, 22 ± 0.4 and 24 ± 0.2, respectively). Major constituents of essential oil determined by GC-MS analysis were tetratriacontane (21.58 %), tetracosane (11.27 %), hexatriacontane (10.19 %), 9-octadecenoic acid, 1,2,3-propanetriyl ester, (E,E,E)- (8.02 %) and heneicosane (7.03 %). Antimicrobial potential of essential oil could have been due to these major constituents.

Keywords

Cichorium intybus Antibacterial activity GC-MS analysis Medicinal plant Antimicrobial activity.
Afzal1, M., Shahid1, M., Mehmood1, Z., Anwer Bukhari2, S., & Munsif Ali Talpur3, M. (2014). Antimicrobial Activity of Extract and Fractions of Different Parts and GC-MS Profiling of Essential Oil of Cichorium intybus Extracted by Super Critical Fluid Extraction. Asian Journal of Chemistry, 26(2), 531–536. https://doi.org/10.14233/ajchem.2014.15667
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References
  1. V. Mulabagal, H. Wang, M. Ngouajio and M.G. Nair, Eur. Food Res. Technol., 230, 47 (2009); doi:10.1007/s00217-009-1144-7.
  2. N. Noor, R.A. Sarfraz, S. Ali and M. Shahid, Food Chem., 143, 362 (2014); doi: 10.1016/j.foodchem.2013.07.084.
  3. A. Hassan, S. Rahman, F. Deeba and S. Mahmud, J. Med. Plants Res., 3, 20 (2009).
  4. N. Mehmood, M. Zubair, K. Rizwan, N. Rasool, M. Shahid and V.U. Ahmad, Iran. J. Pharm. Res., 11, 1145 (2012).
  5. M. Riaz, N. Rasool, I.H. Bukhari, M. Zubair, M. Shahid, T.H. Bokhari, Y. Gull, K. Rizwan, M. Iqbal and M. Zia-Ul-Haq, Oxid. Commun., 36, 542 (2013).
  6. B.M. Schmidt, N. Ilic, A. Poulev and I. Raskin, Food Chem. Toxicol., 45, 1131 (2007); doi:10.1016/j.fct.2006.12.019.
  7. P. Nandagopal and B. Ranjitha, Adv. Biol. Res., 1, 17 (2007).
  8. A. Judzentiene and J. Budiene, Chemija, 19, 25 (2008).
  9. B. Hazra, R. Sarkar, S. Bhattacharyya and P. Roy, Fitoterapia, 73, 730 (2002); doi:10.1016/S0367-326X(02)00232-0.
  10. J.H. Kim, E.J. Lee, D.O. Shin, S.E. Hong and J.K. Kim, J. Korean Assoc. Radiat. Prot., 25, 37 (2000).
  11. P.M. Davidson and A.L. Branen, J. Food Prot., 44, 623 (1981).
  12. T.A. Bischoff, C.J. Kelley, Y. Karchesy, M. Laurantos, P. Nguyen-Dinh and A.G. Arefi, J. Ethnopharmacol., 95, 455 (2004); doi:10.1016/j.jep.2004.06.031.
  13. E. Reverchon, J. Supercrit. Fluids, 10, 1 (1997); doi:10.1016/S0896-8446(97)00014-4.
  14. E.M. Abdallah and A.E. Khalid, Int. J. Chem. Biochem. Sci., 1, 1 (2012).
  15. S. Ahmad, M. Akhter, M. Zia-Ul-Haq, Mehjabeen and S. Ahmed, Pak. J. Bot., 42, 1327 (2010).
  16. A.A. Hamid and O.O. Aiyelaagbe, Int. J. Chem. Biochem. Sci., 1, 99 (2012).
  17. M.A. Hanif, M.Y. Al-maskari, A. Al-maskari, A. Al-shukaili, A.Y. Al-maskari and J.N. Al-sabahi, J. Med. Plants Res., 5, 751 (2011).
  18. M. Lateef, L. Iqbal, N. Fatima, K. Siddiqui, N. Afza, M. Zia-ul-Haq and M. Ahmad, Pak. J. Pharm. Sci., 25, 99 (2012).
  19. K. Rehman, S. Ashraf, U. Rashid, M. Ibrahim, S. Hina, T. Iftikhar and S. Ramzan, Pak. J. Bot., 45, 391 (2013).
  20. Sobia, M. Zubair, N. Rasool, M. Asim, F. Anjum, M. Iqbal, M. Mushtaq and M. Shahid, J. Med. Plants Res., 7, 243 (2012).
  21. M. Zia-Ul-Haq, S. Ahmad, S. Iqbal, D.L. Luthria and R. Amarowicz, Oxid. Commun., 34, 820 (2011).
  22. E. Derwich, Z. Benziane and R. Taouil, Chem. Bull. Politehnica Univ. Timisoara, Romania, 55, 103 (2010).
  23. G.D. Diomande, A.M. Koffi, Z.F. Tonzibo, G. Bedi and G. Figueredo, Middle-East J. Scientific Res., 11, 808 (2012).
  24. M. Pavel, V. Radulescu and D.C. Ilies, Farmacia, 57, 479 (2009).
  25. M. Govindarajan, A. Jebanesan, D. Reetha, R. Amsath, T. Pushpanathan and K. Samidurai, Eur. Rev. Med. Pharmacol. Sci., 12, 299 (2008).
  26. M. Riaz, N. Rasool, I.H. Bukhari, M. Shahid, M. Zubair, K. Rizwan and U. Rashid, Molecules, 17, 14275 (2012); doi:10.3390/molecules171214275.
  27. B. Moosmann, S. Kneisel, U. Girreser, V. Brecht, F. Westphal and V. Auwärter, Forensic Sci. Int., 220, e17 (2012); doi:10.1016/j.forsciint.2011.12.010.
  28. M. Riaz, N. Rasool, I. Bukhari, M. Shahid, F. Zahoor, M. Gilani and M. Zubair, Afr. J. Microbiol. Res., 6, 5700 (2012).
  29. D. Cossuta, B. Simandi, E. Vagi, J. Hohmann, A. Prechl, E. Lemberkovics, A. Kery and T. Keve, J. Supercrit. Fluids, 47, 188 (2008); doi:10.1016/j.supflu.2008.07.014.
  30. B. Mahesh and S. Satish, World J. Agric. Sci., 4, 839 (2008).
  31. F. Anjum, M. Shahid, S.A. Bukhari, S. Anwar and S. Latif, J.Food Process. Technol., 2, 4 (2013).
  32. S.D. Sarker, L. Nahar and Y. Kumarasamy, Methods, 42, 321 (2007); doi:10.1016/j.ymeth.2007.01.006.
  33. D. Mares, C.B. Romagnoli, B. Tosi and E. Andreotti, Fragrance, 13, 235 (2005).
  34. J. Petrovic, A. Stanojkovic, L.J. Comic and S. Curcic, Fitoterapia, 75, 737 (2004); doi:10.1016/j.fitote.2004.05.001.
  35. P. Nandagopal and B. Ranjitha, Adv. Biol. Res., 1, 17 (2007).
  36. T. Sheikh, R.A. Rub and S. Sasikumar, Arabian J. Chem., (2012); doi: 10.1016/j.arabjc.2012.04.012.
  37. N. Mehmood, M. Zubair, K. Rızwan, N. Rasool, M. Shahid and V. Ahmad, Iran. J. Pharm. Res., 11, 1145 (2012).
  38. F. Benbelaid, M. Bendahou, A. Khadir, M.A. Abdoune, C. Bellahsene, F. Zenati, W. Bouali and D.E. Abdelouahid, J. Microbiol. Biotechnol. Res.,2, 244 (2012).
  39. M. Vasinauskiene, J. Radusiene, I. Zitikaite and E. Surviliene, Agronomy Res., 4, 437 (2006).
  40. S.Suganya, R. Bharathidasan, G. Senthilkumar, P. Madhanra and A. Panneerselvam, J. Chem. Pharm. Res., 4, 1846 (2012).
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