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Vol. 26 No. 2 (2014): Vol 26 Issue 2

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Composition of Extracellular Polymeric Substances Produced by Geotrichum sp. J-5

https://doi.org/10.14233/ajchem.2014.16050
YingXin Wan*
The College of Arts and Sciences of Beijing Union University, Beijing 100083, P.R. China *Corresponding author: Tel/Fax: +86 10 62004523; E-mail: wyx@buu.edu.cn
LiPing Wu
The College of Arts and Sciences of Beijing Union University, Beijing 100083, P.R. China *Corresponding author: Tel/Fax: +86 10 62004523; E-mail: wyx@buu.edu.cn
PeiJing Tang
The College of Arts and Sciences of Beijing Union University, Beijing 100083, P.R. China *Corresponding author: Tel/Fax: +86 10 62004523; E-mail: wyx@buu.edu.cn
JingYi Zhang
The College of Arts and Sciences of Beijing Union University, Beijing 100083, P.R. China *Corresponding author: Tel/Fax: +86 10 62004523; E-mail: wyx@buu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 2 (2014): Vol 26 Issue 2

Abstract

A fungus strain (J-5) which produces flocculating substances was isolated from activated sludge and identified as Geotrichum candidum. The growth of the isolated Geotrichum candidum was maximal after 3 days cultivation while the highest flocculating activity of the culture broth was highest after 1 day cultivation. The results of flocculating activity for Geotrichum candidum showed that the active ingredient of bioflocculant was existed in the supernatant of free-cell. The crude bioflocculant could be recovered from the supernatant of the culture broth by ethanol precipitation and dialysis against deionized water. The polymer was identified to be polysaccharide containing neutral sugar and uronic acid as its major and minor components, respectively. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl groups in its molecules which could flocculate by bridging.

Keywords

Bioflocculant Composition Geotrichum candidum Flocculating activity.
Wan*, Y., Wu, L., Tang, P., & Zhang, J. (2014). Composition of Extracellular Polymeric Substances Produced by Geotrichum sp. J-5. Asian Journal of Chemistry, 26(2), 592–596. https://doi.org/10.14233/ajchem.2014.16050
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