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Composition of Extracellular Polymeric Substances Produced by Geotrichum sp. J-5
Asian Journal of Chemistry,
Vol. 26 No. 2 (2014): Vol 26 Issue 2
Abstract
A fungus strain (J-5) which produces flocculating substances was isolated from activated sludge and identified as Geotrichum candidum. The growth of the isolated Geotrichum candidum was maximal after 3 days cultivation while the highest flocculating activity of the culture broth was highest after 1 day cultivation. The results of flocculating activity for Geotrichum candidum showed that the active ingredient of bioflocculant was existed in the supernatant of free-cell. The crude bioflocculant could be recovered from the supernatant of the culture broth by ethanol precipitation and dialysis against deionized water. The polymer was identified to be polysaccharide containing neutral sugar and uronic acid as its major and minor components, respectively. Infrared spectra showed the presence of hydroxyl, carboxyl and methoxyl groups in its molecules which could flocculate by bridging.
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- I.L. Shih, Y.T. Van, L.C. Yeh, H.G. Lin and Y.N. Chang, Bioresour. Technol., 78, 267 (2001); doi:10.1016/S0960-8524(01)00027-X.
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- R.E. Buchanan and N.E. Gibbens, Bergey's Manual of Systematic Bacteriology, Science Press, Beijing, edn 8, (1984).
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- B.L. Kellems and L.W. Lion, Estuar. Coast. Shelf Sci., 28, 443 (1989); doi:10.1016/0272-7714(89)90091-7.
- M.F. Chaplin and J.F. Kennedy, Carbohydrate Analysis, Oxford University Press, New York, edn 2 (1994).
- M.M. Bradford, Anal. Biochem., 72, 248 (1976); doi:10.1016/0003-2697(76)90527-3.
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R. Kurane and Y. Nohata, Agric. Biol. Chem., 55, 1127 (1991); doi:10.1271/bbb1961.55.1127.
V. Crescenzi, Biotechnol. Progr., 11, 251 (1995); doi:10.1021/bp00033a002.
S.H. Yoon, J.K. Song, S.J. Go and J.C. Ryu, J. Biosci. Bioeng., 8, 606 (1998).
J. Nakamura, S. Miyashiro and Y. Hirose, Agric. Biol. Chem., 40, 1341 (1976); doi:10.1271/bbb1961.40.1341.
B. Lian, Y. Chen, J. Zhao, H.H. Teng, L.J. Zhu and S. Yuan, Bioresour. Technol., 99, 4825 (2008); doi:10.1016/j.biortech.2007.09.045.
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C.G. Kumar, H.-S. Joo, J.-W. Choi, Y.-M. Koo and C.-S. Chang, Enzyme Microb. Technol., 34, 673 (2004); doi:10.1016/j.enzmictec.2004.03.001.
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R. Kurane, K. Hatamochi, T. Kakuno, M. Kiyohara, M. Hirano and Y. Taniguchi, Biosci. Biotechnol. Biochem., 58, 428 (1994); doi:10.1271/bbb.58.428.
D.L. Feng and S.H. Xu, World J. Microbiol. Biotechnol., 24, 1627 (2008); doi:10.1007/s11274-008-9654-1.
S.B. Deng, R. Bai and J.P. Chen, J. Colloid Interf. Sci., 260, 265 (2003); doi:10.1016/S0021-9797(02)00243-6.
N. He, Y. Li and J. Chen, Bioresour. Technol., 94, 99 (2004); doi:10.1016/j.biortech.2003.11.013.
R.E. Buchanan and N.E. Gibbens, Bergey's Manual of Systematic Bacteriology, Science Press, Beijing, edn 8, (1984).
T.J. White, T. Bruns, S. Lee and J. Taylor, in eds.: M.A. Innis, D.H. Gelfand, J.J. Sninsky, and T.J. White, Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, PCR Protocols: A Guide to Methods and Applications, Academic Press, New York, p. 315 (1990).
M. Mandel, L. Igambi, J. Bergendahl, M.L. Dodson and E. Scheltgen, J. Bacteriol., 101, 333 (1970).
R. Kurane, K. Takeda and T. Suzuki, Agric. Biol. Chem., 50, 2301 (1986); doi:10.1271/bbb1961.50.2301.
B.L. Kellems and L.W. Lion, Estuar. Coast. Shelf Sci., 28, 443 (1989); doi:10.1016/0272-7714(89)90091-7.
M.F. Chaplin and J.F. Kennedy, Carbohydrate Analysis, Oxford University Press, New York, edn 2 (1994).
M.M. Bradford, Anal. Biochem., 72, 248 (1976); doi:10.1016/0003-2697(76)90527-3.
W.Y. Lu, T. Zhang, D.Y. Zhang, C.H. Li, J.P. Wen and L.X. Du, Biochem. Eng. J., 27, 1 (2005); doi:10.1016/j.bej.2005.04.026.
W.X. Gong, S.G. Wang, X.F. Sun, X.W. Liu, Q.Y. Yue and B.-Y. Gao, Bioresour. Technol., 99, 4668 (2008); doi:10.1016/j.biortech.2007.09.077.
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