Phytochemical Spectrum of Essential Oil of Paganum harmala by GC-MS and Antimicrobial Activity Using Sequential Solvents Fractions and Essential Oil

Muhammad Afzal; Muhammad Shahid*; Amer Jamil

doi:10.14233/ajchem.2014.15998

Issue

Vol. 26 No. 2 (2014): Vol 26 Issue 2

Issue Published : January 15, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Phytochemical Spectrum of Essential Oil of Paganum harmala by GC-MS and Antimicrobial Activity Using Sequential Solvents Fractions and Essential Oil

https://doi.org/10.14233/ajchem.2014.15998

Muhammad Afzal

Department of Chemistry and Biochemistry, Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com

Muhammad Shahid*

Department of Chemistry and Biochemistry, Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com

Amer Jamil

Department of Chemistry and Biochemistry, Institute of Microbiology, University of Agriculture, Faisalabad-38040, Pakistan *Corresponding author: Tel: +92 333 6629271; E-mail: mshahiduaf@yahoo.com

Asian Journal of Chemistry, Vol. 26 No. 2 (2014): Vol 26 Issue 2
Article Published : January 16, 2014

Abstract

Present study was designed to evaluate the antimicrobial potential of essential oil and extracts of different parts of Paganum harmala. Methanolic extract was prepared and extracted further using different organic solvents n-hexane, chloroform, ethyl acetate, n-butanol and water. These extracts were then used for determining their antimicrobial potential. The sample found conspicuous antimicrobial activity of different extracts. In addition, extracted essential oil of this plant using super critical fluid extraction show antimicrobial activity. In essential oil, 1,2-bis[1-(2-hydroxyethyl)-3,6-diazahomoadamantantydene-9]hydrazine (13.29 %), methanol, [4-(1,1-dimethylethyl)phenoxy], acetate (11.29 %), Uridine, 5-tr hexyl-idecafluoro (8.56 %), Tetracosane (5 .40 %) and bis[phenylsulfonyl]-4-trichloromethylphenyl chloromethane (5.29 %) were the major constituents. Antimicrobial potential of P. harmala could be attributed to the compounds detected in essential oil.

Keywords

Paganum harmala Antibacterial activity GC-MS analysis Medicinal plants.

Afzal, M., Shahid*, M., & Jamil, A. (2014). Phytochemical Spectrum of Essential Oil of Paganum harmala by GC-MS and Antimicrobial Activity Using Sequential Solvents Fractions and Essential Oil. Asian Journal of Chemistry, 26(2), 574–578. https://doi.org/10.14233/ajchem.2014.15998

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M. Riaz, N. Rasool, I.H. Bukhari, M. Shahid, M. Zubair, K. Rizwan and U. Rashid, Molecules, 17, 14275 (2012a); doi:10.3390/molecules171214275.

F. Aslam, N. Rasool, M. Riaz, M. Zubair, K. Rizwan, M. Abbas, T.H. Bukhari and I.H. Bukhari, Int. J. Phytomed., 3, 567 (2011).

N. Rasool, H. Tehseen, M. Riaz, K. Rizwan, M. Zubair, Y. Mahmood, M. Iqbal and I.H. Bukhari, Int. J. Chem. Bichem. Sci., 3, 34 (2013).

M. Riaz, N. Rasool, I.H. Bukhari, M. Zubair, M. Shahid, T.H. Bokhari, Y. Gull, K. Rizwan, M. Iqbal and M. Zia-Ul-Haq, Oxi. Commun., 36, 542 (2013).

H. Shao, X. Huang, Y. Zhang and C. Zhang, Molecules, 18, 2623 (2013); doi:10.3390/molecules18032623.

B. Mahesh and S. Satish, World J. Agricultural Sciences., 4, 839 (2008).

E. Darabpour, H. Motamedi, A. Poshtkouhian Bavi, S. Nejad and S. Mansour, EXCLI Journal., 10, 252 (2011).

E. Reverchon, J. Supercrit. Fluids, 10, 1 (1997); doi:10.1016/S0896-8446(97)00014-4.

A.B. Aliyu, M.A. Ibrahim, A.M. Musa, H. Ibrahim, I.E. Abdulkadir and A.O. Oyewale, J. Med. Plants Res., 3, 563 (2009).

S. Adeel, F. Rehman, T. Gulzar, I.A. Bhatti, S. Qaiser and A. Abid, Asian J. Chem., 25, 2739 (2013).

F.- Rehman, S. Adeel, S. Qaiser, I. Ahmad Bhatti, M. Shahid and M. Zube, Radiat. Phys. Chem., 81, 1752 (2012); doi:10.1016/j.radphyschem.2012.06.013.

F. Batool, S. Adeel, M. Azeem, A.A. Khan, I.A. Bhatti, A. Ghaffar and N. Iqbal, Radiat. Phys. Chem., 89, 33 (2013). doi: 10.1016/j.radphyschem.2013.03.045

K. Rehman, S. Ashraf, U. Rashid, M. Ibrahim, S. Hina, T. Iftikhar and S. Ramzan, Pak. J. Bot., 45, 391 (2013).

D. Cossuta, B. Simandi, E. Vagi, J. Hohmann, A. Prechl, E. Lemberkovics, A. Kery and T. Keve, J. Supercrit. Fluids, 47, 188 (2008); doi:10.1016/j.supflu.2008.07.014.

A. Delazar, F. Biglari, S. Esnaashari, H. Nazemiyeh, A.-H. Talebpour, L. Nahar and S.D. Sarker, Phytochem., 67, 2176 (2006); doi:10.1016/j.phytochem.2006.06.012.

A. Sarpeleh, K. Sharifi and A. Sonbolkar, J. Plant Dis. Prot., 5, 208 (2009).

S.D. Sarker, L. Nahar and Y. Kumarasamy, Methods, 42, 321 (2007); doi:10.1016/j.ymeth.2007.01.006.

M. Riaz, N. Rasool, I. Bukhari, M. Shahid, F. Zahoor, M. Gilani and M. Zubair, Afr. J. Microbiol. Res., 6, 5700 (2012).

V. Kumar, T. Shah, N.S. Parmar, G.B. Shah and R.K. Goyal, Indian J. Nat. Prod., 21, 24 (2005).

J. Petrovic, A. Stanojkovic, Lj Comic and S. Curcic, Fitoterapia, 75, 737 (2004); doi: 10.1016/j.fitote.2004.05.001

G. Nenaah, Fitoterapia, 81, 779 (2010); doi:10.1016/j.fitote.2010.04.004.

D.J. Bibel, R. Aly, C. Bayles, W.G. Strauss, H.R. Shinefield and H.I. Maibach, Can. J. Microbiol., 29, 700 (1983); doi:10.1139/m83-114.

K. Rizwan, M. Zubair, N. Rasool, M. Riaz, M. Zia-Ul-Haq and V. de Feo, Int. J. Mol. Sci., 13, 6440 (2012); doi:10.3390/ijms13056440.

Issue

Vol. 26 No. 2 (2014): Vol 26 Issue 2

Phytochemical Spectrum of Essential Oil of Paganum harmala by GC-MS and Antimicrobial Activity Using Sequential Solvents Fractions and Essential Oil

Abstract

Keywords

Full Article

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