Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract

G. Dayana Jeyaleela; J. Rosaline Vimala; R. Senthil; P. Anandagopu; K. Manjula

doi:10.14233/ajchem.2019.22217

Issue

Vol. 31 No. 11 (2019): Vol 31 Issue 11

Issue Published : September 28, 2019

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract

https://doi.org/10.14233/ajchem.2019.22217

G. Dayana Jeyaleela

PG and Research Department of Chemistry, Holy Cross College (Autonomous), Tiruchirappalli-620002, India

https://orcid.org/0000-0001-5038-4389

J. Rosaline Vimala

PG and Research Department of Chemistry, Holy Cross College (Autonomous), Tiruchirappalli-620002, India

https://orcid.org/0000-0003-1245-3560

R. Senthil

Lysine Biotech Private Limited, Periyar Maniammai Institute of Science and Technology, Thanjavur-613403, India & Faculty in Bioinformatics, Marudupandiyar Institutions, Thanjavur-613403, India

https://orcid.org/0000-0002-8451-9832

P. Anandagopu

Lysine Biotech Private Limited, Periyar Maniammai Institute of Science and Technology, Thanjavur-613403, India & Faculty in Bioinformatics, Marudupandiyar Institutions, Thanjavur-613403, India

https://orcid.org/0000-0002-0228-1745

K. Manjula

Bio Techno Solutions Training and Research Institute, Tiruchirappalli-620020, India

https://orcid.org/0000-0002-2481-256X

Corresponding Author(s) : G. Dayana Jeyaleela

princedayana1221@gmail.com, dayana@hcctrichy.ac.in

Asian Journal of Chemistry, Vol. 31 No. 11 (2019): Vol 31 Issue 11
Article Published : September 28, 2019

Abstract

Present work aimed to isolate bioactive principle and its inhibitory potential against struvite stone by single diffusion method as well as docking studies to determine the mode of interaction between the complex structure of urease and rutin. Flavonoid compound is isolated from Melia dubia leaf extract with the help of TLC and column chromatography, and then characterized by ¹H & ¹³C NMR, and LC-MS. Inhibitory potential of isolated flavonoid against kidney stone (struvite) is confirmed by molecular docking approach. Rutin acts as a urease inhibitor and the residues of urease namely 463Asp and 466 Ala were found to bind with rutin through hydrogen bonding and van der Waals interactions. In vitro study, the results revealed that with an increase in concentration of flavonoid rutin (1-5 mg/mL) the weight of the harvested crystals were gradually reduced from 2.8 cm to 0.5 cm and weight 0.662 g to 0.322 g at 5 mg/mL concentration. The XRD result of C+5 shows well inhibition on struvite crystal in all the planes and in SEM analysis, rutin inhibited crystal, surface of the crystal was damaged, whereas control shows pure and no defects on it.

Keywords

Melia dubia Rutin Struvite stone Molecular Docking Single diffusion method

Jeyaleela, G. D., Vimala, J. R., Senthil, R., Anandagopu, P., & Manjula, K. (2019). Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract. Asian Journal of Chemistry, 31(11), 2628–2634. https://doi.org/10.14233/ajchem.2019.22217

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References

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References

R. Flannigan, W.H. Choy, B. Chew and D. Lange, Nat. Rev. Urol., 11, 333 (2014); https://doi.org/10.1038/nrurol.2014.99.

L. Benramdane, M. Bouatia, M.O.B. Idrissi and M. Draoui, Spectrosc. Lett., 41, 72 (2008); https://doi.org/10.1080/00387010801943806.

V. Butterweck and S.R.X. Khan, Planta Med., 75, 1095 (2009); https://doi.org/10.1055/s-0029-1185719.

P. Das, G. Gupta, V. Velu, R. Awasthi, K. Dua and H. Malipeddi, Biomed. Pharmacother., 96, 361 (2017); https://doi.org/10.1016/j.biopha.2017.10.015.

G. Mandel and N. Mandel, eds.: F.L. Coe, M.J. Favus, C.Y.C. Pak, J.H. Parks and G.M. Preminger, Analysis of Stones, In: Kidney Stones, Medical and Surgical Management, Lippincott-Raven: Philadelphiapp. 323-335 (1986).

F. Deng and J.M. Ouyang, Mater. Sci. Eng. C, 26, 688 (2006); https://doi.org/10.1016/j.msec.2005.06.061.

M.A.P. Manzoor, B. Singh, A.K. Agrawal, A.B. Arun, M. Mujeeburahiman and P.-D. Rekha, PLoS One, 13, e0202306 (2018); https://doi.org/10.1371/journal.pone.0202306.

M. Biocic, M. Saraga, A. Cvitkovic Kuzmic, Z. Bahtijarevic, D. Budimir, J. Todoric and R.M. Ujevic, Coll. Antropol., 27, 745 (2003).

H. Kuvezdic, A. Tucak, N. Peric, D. Prlic, I. Zoric and R. Galic, Coll. Antropol., 27, 71 (2003).

K.H. Bichler, E. Eipper, K. Naber, V. Braun, R. Zimmermann and S. Lahme, Int. J. Antimicrob. Agents, 19, 488 (2002); https://doi.org/10.1016/S0924-8579(02)00088-2.

S. Muryanto, S. Sutanti and M. Kasmiyatun, Inhibition of Struvite Crystal Growth in the Presence of Herbal Extract orthosiphon aristatus BL.MIQ. MATEC web of conferences, 01013, pp. 1-5 (2016).

A. Torzewska and A. Rozalski, Microbiol. Res., 169, 579 (2014); https://doi.org/10.1016/j.micres.2013.09.020.

M. Beghalia, H. Allali, S. Ghalem, A. Belouatek and A. Sari, Adv. J. Mol. Imaging, 1, 33 (2011); https://doi.org/10.4236/ami.2011.13005.

G.D. Jeyaleela, N. Balasubramani and J.R. Vimala, Asian J. Pharm. Clin. Res., 12, 107 (2019); https://doi.org/10.22159/ajpcr.2019.v12i2.28384.

N. Raaman, Phytochemical Techniques, New India Publication Agency: New Delhi, pp. 275-286 (2006).

J.B. Harborne, A Guide to Modern Techniques of Plant Analysis, Kluwer Academic Publisher: USA, edn 2, pp. 75-77 (1998).

M.A.A. Abdullah, G.E.-D.A.A. Abuo-Rahma, E.-S.M.N. Abdelhafez, H.A. Hassan and R.M. Abd El-Baky, Bioorg. Chem., 70, 1 (2017); https://doi.org/10.1016/j.bioorg.2016.11.002.

A.A. Mohammed, Afr. J. Microbiol. Res., 10, 81 (2016).

K. Suguna, M. Thenmozhi and C. Sekar, Bull. Mater. Sci., 35, 701 (2012); https://doi.org/10.1007/s12034-012-0322-6.

J. Prywer and A. Torzewska, Evid. Based Complement Alternat. Med., 2012, Article ID 862794 (2012); https://doi.org/10.1155/2012/862794.

C.K. Chauhan and M.J. Joshi, J. Cryst. Growth, 362, 330 (2013); https://doi.org/10.1016/j.jcrysgro.2011.11.008.

M. Das, H. Malipeddi, N.A. Nambiraj and R. Rajan, J. Food Biochem., 40, 148 (2016); https://doi.org/10.1111/jfbc.12205.

Issue

Vol. 31 No. 11 (2019): Vol 31 Issue 11

Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract

Corresponding Author(s) : G. Dayana Jeyaleela

Abstract

Keywords

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