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Vol. 31 No. 11 (2019): Vol 31 Issue 11

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Entrapment of Glucose Oxidase in Reverse Micelle Microemulsion Systems for Glucose Detection in Lipid Based Food Products

https://doi.org/10.14233/ajchem.2019.22260
Lovnish Siyal
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India
Benu Kumar
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India
Arpita Bhattacharya
Amity Institute of Nanotechnology, Amity University Uttar Pradesh, Noida-201313, India
Rachana Sahney
Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida-201313, India

Corresponding Author(s) : Rachana Sahney

rachanasahney@gmail.com, rsahney@amity.edu

Asian Journal of Chemistry, Vol. 31 No. 11 (2019): Vol 31 Issue 11

Abstract

Entrapment of glucose oxidase (GOx) enzyme in a new reverse micelle emulsion system was studied. The microemulsion consists of aqueous phase (buffered enzyme)/SPAN 85/n-decane. Critical micelle concentration (CMC) of surfactant-SPAN 85 in n-decane was determined using dynamic light scattering study and it was used to develop microemulsion system. Most stable and optically transparent microemulsion with entrapped glucose oxidase showed higher values of specific enzyme activity, maximum reaction rate (Vmax) and turn over number and low value of Michaelis-constant (Km) in comparison to homogeneous GOx (enzyme-glucose oxidase) system. The microemulsion system was successfully used to quantify D-glucose in lipid based food products without any sample preparation. Comparison of these results with chemical method (phenol-sulfuric acid method) and commercial kit method used in food industry validate the efficiency of the new proposed system. The study provides new information about the glucose content of some commonly consumed milk based products where nutritional labels do not accurately show true glucose content. These findings provide support for comprehensive glucose labeling to food products commonly used by the children.

Keywords

Enzyme kinetics Food Microemulsion Reverse micelle
Siyal, L., Kumar, B., Bhattacharya, A., & Sahney, R. (2019). Entrapment of Glucose Oxidase in Reverse Micelle Microemulsion Systems for Glucose Detection in Lipid Based Food Products. Asian Journal of Chemistry, 31(11), 2635–2641. https://doi.org/10.14233/ajchem.2019.22260
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