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Vol. 26 No. 7 (2014): Vol 26 Issue 7

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Determination of Fatty Acid and Essential Oil Constituents and Biological Activities on Ranunculus pedatus Subsp. pedatus

https://doi.org/10.14233/ajchem.2014.16762
T. Erdogan
Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey
T. Gonenc
Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey
B. Demirci
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey
B. Kivcak
Department of Pharmacognosy, Faculty of Pharmacy, Ege University, Bornova, Izmir, Turkey
K.H.C. Baser
Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskisehir, Turkey; Botany and Microbiology Department, College of Science, King Saud University, Riyadh, Saudi Arabia; Technology Transfer Office, Bahcesehir University, Istanbul, Turkey

Corresponding Author(s) : T. Erdogan

tugce.fafal@ege.edu.tr; tugceerfa@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 7 (2014): Vol 26 Issue 7

Abstract

In this study, the fatty-acid profile, essential-oil composition and cytotoxic and antioxidant activities of the aerial parts of Ranunculus pedatus Waldst. & Kit. subsp. pedatus have been examined. Linoleic acid (24.47 %) and palmitoleic acid (18.94 %) were identified as major components by GC-FID and GC/MS analysis. Unsaturated fatty acids were found in higher amounts than saturated fatty acids. The essential oil composition was also analyzed by GC and GC/MS and 29 components were characterized. Protoanemonin (14.3 %), phytol (13.7 %), a-bisabolol (7.9 %), (Z)-3-hexenol (7.8 %) and octanol (6.7 %) were determined as the main constituents of the essential oil. Ethanol and chloroform extracts were investigated for the cytotoxic activity against PC3, HeLa and Vero human cancer cell lines and both of the extracts were found to be effective on HeLa. The IC50 values were determined as 47 and 78.4 mg/mL for methanol and chloroform extracts respectively. Antioxidant activity of chloroform and ethanol extracts were evaluated by DPPH assay. The total phenolics and flavonoid contents of the extracts were determined by Folin-Ciocalteau and aluminium chloride methods, respectively. The ethanol extract was found to possess 67.54 % DPPH inhibition. The results obtained in the antioxidant activity assay were in positive correlation with the total phenolics and flavonoid contents of the extracts.

Keywords

Ranunculus pedatus subsp. pedatus Fatty acid Essential oil Cytotoxic activity Antioxidant activity
Erdogan, T., Gonenc, T., Demirci, B., Kivcak, B., & Baser, K. (2014). Determination of Fatty Acid and Essential Oil Constituents and Biological Activities on Ranunculus pedatus Subsp. pedatus. Asian Journal of Chemistry, 26(7), 2156–2160. https://doi.org/10.14233/ajchem.2014.16762
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