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Determination of Organic Acid Composition and Free Radical Scavenging Capacity of Kefir
Corresponding Author(s) : Gulen Turker
Asian Journal of Chemistry,
Vol. 26 No. 8 (2014): Vol 26 Issue 8
Abstract
The organic acid composition as well as the free radical scavenging capacity of kefir produced from different kinds of milk was investigated. The organic acid composition of the samples was determined by high performance liquid chromatography (HPLC), while free radical scavenging capacity was evaluated spectrophotometrically. Oxalic acid, acetic acid, citric acid and succinic acids were found to be significantly higher in kefir produced from cow milk (p < 0.05), while malic acid and lactic acid were significantly higher in kefir produced from goat milk (p < 0.05). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity of kefir produced from goat milk was significantly higher than that of kefir made from cow milk (p < 0.05). There was a high correlation between the malic acid and lactic acid formation and the free radical scavenging activity of the samples, implying that the formation of these organic acids during kefir production positively enhances the free radical scavenging capacity of the fermented milk products.
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- Z.B. Guzel-Seydim, T. Kok-Tas, A.K. Greene and A.C. Seydim, Crit. Rev. Food Sci. Nutr., 51, 261 (2011); doi:10.1080/10408390903579029.
- S. Sarkar, Br. Food J., 109, 280 (2007); doi:10.1108/00070700710736534.
- S. Otles and O. Cagindi, Pak. J. Nutr., 2, 54 (2003); doi:10.3923/pjn.2003.54.59.
- A.A. Ismaiel, M.F. Ghaly and A.K. El-Naggar, J. Sci. Ind. Res. (India), 70, 365 (2011).
- K.T. Magalhães, G. Dragone, G.V. de Melo Pereira, J.M. Oliveira, L. Domingues, J.A. Teixeira, J.B.A. e Silva and R.F. Schwan, Food Chem., 126, 249 (2011); doi:10.1016/j.foodchem.2010.11.012.
- G.L. Garrote, A.G. Abraham and G.L. De Antoni, J. Dairy Res., 68, 639 (2001); doi:10.1017/S0022029901005210.
- M.C. García Fontán, S. Martínez, I. Franco and J. Carballo, Int. Dairy J., 16, 762 (2006); doi:10.1016/j.idairyj.2005.07.004.
- T. Ozcan, Asian J. Chem., 22, 6589 (2010).
- Z. Ahmed, Y. Wang, A. Ahmad, S.T. Khan, M. Nisa, H. Ahmad and A. Afreen, Crit. Rev. Food Sci. Nutr., 53, 422 (2013); doi:10.1080/10408398.2010.540360.
- J.L. Evans, I.D. Goldfine, B.A. Maddux and G.M. Grodsky, Diabetes, 52, 1 (2003); doi:10.2337/diabetes.52.1.1.
- B. Halliwell, J.M.C. Gutteridge and C.E. Cross, J. Lab. Clin. Med., 119, 598 (1992).
- C. Lopez-Alarcon and A. Denicola, Anal. Chim. Acta, 763, 1 (2013); doi:10.1016/j.aca.2012.11.051.
- G.F.W. Haenlein, Small Rumin. Res., 51, 155 (2004); doi:10.1016/j.smallrumres.2003.08.010.
- J.R. Liu, Y.Y. Lin, M.J. Chen, L.J. Vhen and C.W. Lin, Asian-Aust. J. Anim. Sci., 18, 567 (2005).
- G. Turker, B. Kizilkaya and N. Cevik, J. Food Agric. Environ., 11, 62 (2013).
- R.T. Marsili, H. Ostapenko, R.E. Simmons and D.E. Green, J. Food Sci., 46, 52 (1981); doi:10.1111/j.1365-2621.1981.tb14529.x.
- Z.B. Guzel-Seydim, A.C. Seydim, A.K. Greene and A.B. Bodine, J. Food Compos. Anal., 13, 35 (2000); doi:10.1006/jfca.1999.0842.
- G.L. Garrote, A.G. Abraham and G.L. De Antoni, J. Food Prot., 63, 364 (2000).
- W.E.V. Lankaputhra and N.P. Shah, Mutat. Res., 397, 169 (1998); doi:10.1016/S0027-5107(97)00208-X.
- M. Arnetoli, G. Montegrossi, A. Buccianti and C. Gonnelli, J. Agric. Food Chem., 56, 789 (2008); doi:10.1021/jf072203d.
- W. Brand-Williams, M.E. Cuvelier and C. Berset, LWT-Food Sci. Technol., 28, 25 (1995); doi:10.1016/S0023-6438(95)80008-5.
- H. Gronnevik, M. Falstad and J.A. Narvhus, Int. Dairy J., 21, 601 (2011); doi:10.1016/j.idairyj.2011.01.001.
- J.M. Lü, P.H. Lin, Q. Yao and C. Chen, J. Cell. Mol. Med., 14, 840 (2010); doi:10.1111/j.1582-4934.2009.00897.x.
- C. Sanchez-Moreno, Food Sci. Technol. Int., 8, 121 (2002).
- R. Lo Scalzo, Food Chem., 107, 40 (2008); doi:10.1016/j.foodchem.2007.07.070.
References
Z.B. Guzel-Seydim, T. Kok-Tas, A.K. Greene and A.C. Seydim, Crit. Rev. Food Sci. Nutr., 51, 261 (2011); doi:10.1080/10408390903579029.
S. Sarkar, Br. Food J., 109, 280 (2007); doi:10.1108/00070700710736534.
S. Otles and O. Cagindi, Pak. J. Nutr., 2, 54 (2003); doi:10.3923/pjn.2003.54.59.
A.A. Ismaiel, M.F. Ghaly and A.K. El-Naggar, J. Sci. Ind. Res. (India), 70, 365 (2011).
K.T. Magalhães, G. Dragone, G.V. de Melo Pereira, J.M. Oliveira, L. Domingues, J.A. Teixeira, J.B.A. e Silva and R.F. Schwan, Food Chem., 126, 249 (2011); doi:10.1016/j.foodchem.2010.11.012.
G.L. Garrote, A.G. Abraham and G.L. De Antoni, J. Dairy Res., 68, 639 (2001); doi:10.1017/S0022029901005210.
M.C. García Fontán, S. Martínez, I. Franco and J. Carballo, Int. Dairy J., 16, 762 (2006); doi:10.1016/j.idairyj.2005.07.004.
T. Ozcan, Asian J. Chem., 22, 6589 (2010).
Z. Ahmed, Y. Wang, A. Ahmad, S.T. Khan, M. Nisa, H. Ahmad and A. Afreen, Crit. Rev. Food Sci. Nutr., 53, 422 (2013); doi:10.1080/10408398.2010.540360.
J.L. Evans, I.D. Goldfine, B.A. Maddux and G.M. Grodsky, Diabetes, 52, 1 (2003); doi:10.2337/diabetes.52.1.1.
B. Halliwell, J.M.C. Gutteridge and C.E. Cross, J. Lab. Clin. Med., 119, 598 (1992).
C. Lopez-Alarcon and A. Denicola, Anal. Chim. Acta, 763, 1 (2013); doi:10.1016/j.aca.2012.11.051.
G.F.W. Haenlein, Small Rumin. Res., 51, 155 (2004); doi:10.1016/j.smallrumres.2003.08.010.
J.R. Liu, Y.Y. Lin, M.J. Chen, L.J. Vhen and C.W. Lin, Asian-Aust. J. Anim. Sci., 18, 567 (2005).
G. Turker, B. Kizilkaya and N. Cevik, J. Food Agric. Environ., 11, 62 (2013).
R.T. Marsili, H. Ostapenko, R.E. Simmons and D.E. Green, J. Food Sci., 46, 52 (1981); doi:10.1111/j.1365-2621.1981.tb14529.x.
Z.B. Guzel-Seydim, A.C. Seydim, A.K. Greene and A.B. Bodine, J. Food Compos. Anal., 13, 35 (2000); doi:10.1006/jfca.1999.0842.
G.L. Garrote, A.G. Abraham and G.L. De Antoni, J. Food Prot., 63, 364 (2000).
W.E.V. Lankaputhra and N.P. Shah, Mutat. Res., 397, 169 (1998); doi:10.1016/S0027-5107(97)00208-X.
M. Arnetoli, G. Montegrossi, A. Buccianti and C. Gonnelli, J. Agric. Food Chem., 56, 789 (2008); doi:10.1021/jf072203d.
W. Brand-Williams, M.E. Cuvelier and C. Berset, LWT-Food Sci. Technol., 28, 25 (1995); doi:10.1016/S0023-6438(95)80008-5.
H. Gronnevik, M. Falstad and J.A. Narvhus, Int. Dairy J., 21, 601 (2011); doi:10.1016/j.idairyj.2011.01.001.
J.M. Lü, P.H. Lin, Q. Yao and C. Chen, J. Cell. Mol. Med., 14, 840 (2010); doi:10.1111/j.1582-4934.2009.00897.x.
C. Sanchez-Moreno, Food Sci. Technol. Int., 8, 121 (2002).
R. Lo Scalzo, Food Chem., 107, 40 (2008); doi:10.1016/j.foodchem.2007.07.070.