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Vol. 26 No. 9 (2014): Vol 26 Issue 9

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Studies on the Interaction Mechanism of Morin-Co(II) with DNA and Preparation of Electrochemical DNA Biosensor

https://doi.org/10.14233/ajchem.2014.15739
Shu-Yan Niu
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
Huan -Huan Cui
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
Xiao-Tong Shen
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China
Ming-Liang Wu
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China

Corresponding Author(s) : Shu-Yan Niu

wenzhangggg@126.com

Asian Journal of Chemistry, Vol. 26 No. 9 (2014): Vol 26 Issue 9

Abstract

Fabrication and electrochemical characterization of a sensitive and novel DNA electrochemical biosensor for the detection of the specific target DNA fragment with a new prepared indicator was presented. The sensor was based on multi-walled carbon nanotubes functionalized with a carboxylic acid group (MWNTs-COOH) as the signal hybridization detection enhancement element. The well dispersed MWNTs were dripped onto the glassy carbon electrode (GCE) surface firstly to prepare MWNTs/GCE. Oligonucleotides with the 5´-amino group were covalently bonded to the carboxyl group of carbon nanotubes. Cyclic voltammetry was used to characterized the modified electrode. The hybridization events were monitored by differential pulse voltammetry (DPV) analysis using a novel electroactive intercalator Morin-Co(II) as an indicator. The specific coliform DNA fragment was detected by the proposed biosensor with a good linear range and a lower detection limit. The linear range is from 2 × 10-9 M to 5 × 10-10 M with a detection limit of 8.1 × 10-11 M (3s, n = 7). Moreover, the sensor presentes excellent stability and a high repeatability.

Keywords

DNA Electrochemisitry Multi-walled carbon nanotubes Morin-Co(II)
Niu, S.-Y., -Huan Cui, H., Shen, X.-T., & Wu, M.-L. (2014). Studies on the Interaction Mechanism of Morin-Co(II) with DNA and Preparation of Electrochemical DNA Biosensor. Asian Journal of Chemistry, 26(9), 2578–2582. https://doi.org/10.14233/ajchem.2014.15739
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