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Vol. 26 No. 9 (2014): Vol 26 Issue 9

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Some Physical and Chemical Characteristics, Molecular Typing of Isolated E. coli Strains of Fountain Waters, Turkey

https://doi.org/10.14233/ajchem.2014.15718
Pinar Sekerci
Department of Food Engineering, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey
Mustafa Gurses
Department of Food Engineering, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey
Bulent Cetin
Department of Food Engineering, Faculty of Agriculture, Ataturk University, 25240 Erzurum, Turkey

Corresponding Author(s) : Mustafa Gurses

mgurses@atauni.edu.tr

Asian Journal of Chemistry, Vol. 26 No. 9 (2014): Vol 26 Issue 9

Abstract

In this study, water samples collected from public fountains in Erzurum city centrum were analyzed and their drinking quality was evaluated. A total of 45 water samples were subjected to microbiological, chemical and physical analyses. Microbiological analysis results showed that total and fecal coliform bacteria were found in 10 samples (22.7 %) and Clostridium perfringens in only one sample. In addition, Escherichia coli were isolated from these 10 samples. RAPD-PCR technique was used for confirming phylogenetic diversity among E. coli species isolated from various fountains. Six of 45 typical E. coli isolates were determined to be different strains. Average colour, water temperature, turbidity, electrical conductivity, pH, dissolved oxygen, chloride, nitrite, nitrate, sulphate, phosphate, sodium, ammonium, potassium, magnesium, calcium, iron and manganese values of the samples were determined as 1.04 mg Pt L-1, 21.43 ºC, 0.14 NTU, 456.80 μS cm-1, 7.26, 5.36, 3029, 0.12, 2.09, 50.48, 22.71, 35.91, 0.32, 14.99, 31.20, 105.00, 0.16 and 2.64 mg L-1, respectively.

Keywords

Fountain water E. coli Coliform bacteria RAPD-CR
Sekerci, P., Gurses, M., & Cetin, B. (2014). Some Physical and Chemical Characteristics, Molecular Typing of Isolated E. coli Strains of Fountain Waters, Turkey. Asian Journal of Chemistry, 26(9), 2518–2522. https://doi.org/10.14233/ajchem.2014.15718
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