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Immobilization of a-Amylase by SBA-15
Corresponding Author(s) : Qing-Zhou Zhai
Asian Journal of Chemistry,
Vol. 26 No. 9 (2014): Vol 26 Issue 9
Abstract
SBA (Santa Barbara Amorphous)-15 mesoporous material was prepared by hydrothermal synthesis and the sample was characterized by X-ray diffraction and scanning electron microscopy. The immobilization of a-amylase by SBA-15 was made by physical adsorption method and the optimal conditions of immobilization of a-amylase by SBA-15 were obtained as follows: pH = 5.4, a-amylase concentration:2 mg/mL, immobilization time:27 h, temperature:30 °C. At the same time, it is concluded that the maximum amount of a-amylase immobilized by SBA-15 was 87.5 mg/g. By studying of chemical analysis, scanning electron microscopy, infrared spectroscopy, X-ray diffraction, low temperature N2 adsorption-desorption analysis characterization, the a-amylase was immobilized on the SBA-15 carrier was proved. Catalytic experimental results indicated that (SBA-15)-(a-amylase) composite material has the advantages of high pH value resistance and high temperature resistance compared with the free enzyme and has a higher potential applied value.
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References
D.Y. Zhao, J.L. Feng, Q.S. Huo, N. Melosh, G.H. Fredrickson, B.F. Chmelka and G.D. Stucky, Science, 279, 548 (1998); doi:10.1126/science.279.5350.548.
D.Y. Zhao, Q.S. Huo, J.L. Feng, B.F. Chmelka and G.D. Stucky, J. Am. Chem. Soc., 120, 6024 (1998); doi:10.1021/ja974025i.
M. Hartmann, Chem. Mater., 17, 4577 (2005); doi:10.1021/cm0485658.
Z.N. Huang, Bull. Chin. Ceram. Soc, 32, 1311 (2013).
J.F. Diaz and K.J. Balkus Jr., J. Mol. Catal. B, 2, 115 (1996); doi:10.1016/S1381-1177(96)00017-3.
H.H.P. Yiu, P.A. Wright and N.P. Botting, Micropor. Mesopor. Mater., 44-45, 763 (2001); doi:10.1016/S1387-1811(01)00258-X.
H. Gustafsson, E.M. Johansson, A. Barrabino, M. Odén and K. Holmberg, Colloids Surf., 100, 22 (2012); doi:10.1016/j.colsurfb.2012.04.042.
J.A. Laszlo, M. Jackson and R.M. Blanco, J. Mol. Catal. B, 69, 60 (2011); doi:10.1016/j.molcatb.2010.12.011.
M. Shakeri and K. Kawakami, Catal. Commun., 10, 165 (2008); doi:10.1016/j.catcom.2008.08.012.
Z. Luan, M. Hartmann, D. Zhao, W. Zhou and L. Kevan, Chem. Mater., 11, 1621 (1999); doi:10.1021/cm9900756.
Q.S. Wang, Q.Z. Zhai and J. Luo, Asian J. Chem., 25, 5470 (2013); doi:10.14233/ajchem.2013.14820.
B. Stellmach, Determination Method of Enzyme, China Light Industry Press, Beijing, p. 29, (1992).
S. Brunauer, P.H. Emmett and E. Teller, J. Am. Chem. Soc., 60, 309 (1938); doi:10.1021/ja01269a023.
E.P. Barrett, L.G. Joyner and P.P. Halenda, J. Am. Chem. Soc., 73, 373 (1951); doi:10.1021/ja01145a126.