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Vol. 26 No. 10 (2014): Vol 26 Issue 10

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Optimization of Kinetic Method for the Estimation of N-Acetyl L-Cysteine and Characterization of its Oxidation and Degradation Product by Mass Spectrometry

https://doi.org/10.14233/ajchem.2014.16077
Masoom Raza Siddiqui
Chemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia
Zeid Abdullah Alothman
Chemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia
Saikh Mohammad Wabaidur
Chemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia
Md. Sajid Ali
College of Pharmacy, Jazan University, Jazan, Saudi Arabia
Md Sarfaraz Alam
College of Pharmacy, Jazan University, Jazan, Saudi Arabia
Taieb Aouak
Chemistry Department, College of Science, King Saud University, Riyadh, Saudi Arabia

Corresponding Author(s) : Masoom Raza Siddiqui

siddiqui124@gmail.com

Asian Journal of Chemistry, Vol. 26 No. 10 (2014): Vol 26 Issue 10

Abstract

A simple kinetic spectrophotometric method has been developed and validated for the quantitative analysis of N-acetyl L-cysteine. The proposed method is based on the oxidation of N-acetyl L-cysteine by potassium permanganate. The mechanism of the reaction was studied and the reaction product was subjected to mass spectrometric analysis for characterization. By the spectral analysis it was concluded that the oxidation of N-acetyl L-cysteine resulted in the formation of a disulfides, sulfinic acid and sulphonic acid. Two methods, initial rate and fixed time were adopted to quantitate the drug. The method was validated as per the ICH guidelines. The calibration graph obtained by the current methods was found to be linear in the range of 3.5-77 μg mL–1. The detection limit of the initial rate and the fixed time methods was calculated to be 8.5 × 10–3 and 0.456, respectively while the quantitation limit came out to be 2.59 × 10–3 and 0.140, respectively for the initial rate and fixed time methods.

Keywords

N-Acetyl L-cysteine Analytical method Degradation product Mass spectrometry
Raza Siddiqui, M., Abdullah Alothman, Z., Mohammad Wabaidur, S., Sajid Ali, M., Sarfaraz Alam, M., & Aouak, T. (2014). Optimization of Kinetic Method for the Estimation of N-Acetyl L-Cysteine and Characterization of its Oxidation and Degradation Product by Mass Spectrometry. Asian Journal of Chemistry, 26(10), 2935–2940. https://doi.org/10.14233/ajchem.2014.16077
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