High Performance Liquid Chromatographic Simultaneous Analysis for Quantitation of Eight Marker Compounds of Traditional Korean Medicine, Ssanghwa-Tang

Abstract

We performed simultaneous quantitation of eight components, 5-(hydroxymethyl)-2-furaldehyde (5-HMF), albiflorin, paeoniflorin, liquiritin, ferulic acid, cinnamic acid, cinnamaldehyde and glycyrrhizin of Ssanghwa-tang using high-performance liquid chromatography-photodiode array. A Luna C18 column was used as the analytical column for separation of the eight compounds. The mobile phase used gradient elution with 1 % (v/v) aqueous acetic acid (A) and 1 % (v/v) acetic acid in acetonitrile (B). The flow rate was 1 mL/min, column temperature was maintained at 40 °C and the injection volume was 10 μL. The PDA detector was monitored in the range 190-400 nm. Calibration curves of the eight compounds showed good linearity (r² ³ 0.9998). The recoveries of the eight compounds were 92.80-103.43 %. The relative standard deviations of intraday and interday precision were 0.07-2.03 and 0.08-1.78 %, respectively. The concentrations of the eight marker components were 0.148-12.261 mg/g.