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Vol. 26 No. 10 (2014): Vol 26 Issue 10

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in vitro and in vivo Evaluation of Novel Ocular Delivery System of 5-Fluorouracil Peptide Hydrogel

https://doi.org/10.14233/ajchem.2014.16220
Liang Liang
Yichang Central People's Hospital, Department of Ophthalmology, Three Gorges University, Yichang 443003, P.R. China
Qing-Hua Li
Yichang Central People's Hospital, Department of Ophthalmology, Three Gorges University, Yichang 443003, P.R. China
Kun Jin
Yichang Central People's Hospital, Department of Ophthalmology, Three Gorges University, Yichang 443003, P.R. China
Tong Luo
Yichang Central People's Hospital, Department of Ophthalmology, Three Gorges University, Yichang 443003, P.R. China
Zhong-Ai Yang
Yichang Central People's Hospital, Department of Ophthalmology, Three Gorges University, Yichang 443003, P.R. China
Xiao-Ding Xu
Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China
Han Cheng
Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, P.R. China

Corresponding Author(s) : Liang Liang

niuniu419519@163.com

Asian Journal of Chemistry, Vol. 26 No. 10 (2014): Vol 26 Issue 10

Abstract

A novel biocompatible hydrogel was prepared based on the supramolecular self-assembly of a peptide containing a bioactive RGD (arginine-glycine-aspartic acid) sequence and a hydrophobic N-fluorenyl-9-methoxycarbonyl (FMOC) tail. The in vitro biocompatibility assay demonstrated that such self-assembled peptide hydrogel had a well biocompatibility. When intraoperatively administrating the self-assembled peptide hydrogel containing 5-fluorouracil in the filtering surgery of rabbit eyes, quantitative determination of 5-fluorouracil in the aqueous humor showed detectable amounts of 5-fluorouracil (below 1μg/mL) for 1 weeks, which shows that peptide hydrogel was still present in the subconjunctival space for that period and released 5-fluorouracil in a continuous fashion. Attributed to the sustained release of 5-fluorouracil from the hydrogel, the injection of the self-assembled peptide hydrogel containing 5-fluorouracil at surgery site had potential advantages including preventing the frequent 5-fluorouracil injections and the toxicity of 5-fluorouracil to the surrounding ocular tissues efficiently, indicating a potential application of the self-assembled peptide hydrogel as an implanted drug delivery system for the treatment of scarring formation after filtering surgery.

Keywords

Self-assembly Peptide hydrogel Filtering Surgery Ocular Delivery system
Liang, L., Li, Q.-H., Jin, K., Luo, T., Yang, Z.-A., Xu, X.-D., & Cheng, H. (2014). in vitro and in vivo Evaluation of Novel Ocular Delivery System of 5-Fluorouracil Peptide Hydrogel. Asian Journal of Chemistry, 26(10), 2977–2981. https://doi.org/10.14233/ajchem.2014.16220
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