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Vol. 26 No. 10 (2014): Vol 26 Issue 10

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Characterization of (SBA-15)-Lipase Composite Material

https://doi.org/10.14233/ajchem.2014.16678
Si-Jia Sun
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, Jilin Province, P.R. China
Qing-Zhou Zhai
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, Jilin Province, P.R. China

Corresponding Author(s) : Qing-Zhou Zhai

zhaiqingzhou@163.com

Asian Journal of Chemistry, Vol. 26 No. 10 (2014): Vol 26 Issue 10

Abstract

Santa Barbara Amorphous (SBA)-15 mesoporous material was synthesized by hydrothermal method using tri-block copolymer poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (Pluronic P123) as a structure-directing agent and then phenyltriethoxysilane was used to to obtain phenylated SBA-15. Then physical adsorptive method was employed to immobilize lipase to the host material. The immobilized efficiency of enzyme was improved to some extent for the phenylated SBA-15. For structural characterization of prepared samples, powder X-ray diffraction, Fourier transform infrared spectroscopy and low temperature N2 adsorption-desorption at 77 K were used. The study confirmed that the enzyme went into the pore channels of the SBA-15. Scanning electron microscopic studies showed that the average particle diameter of (SBA-15)-lipase was 337 ± 10 nm, while the average particle diameter of (phenylated SBA-15)-lipase was 340 ± 10 nm. Luminescent spectra showed that the enzyme had already successfully been immobilized to the host materials and after the enzyme was immobilized into host materials the configuration had not been changed. The experimental results of activity on the hydrolysis of glycerol triacetate catalyzed by lipase indicated that compared the immobilized lipase with free lipase the catalytic activity was enhanced and the catalytic use rate of lipase was improved and the ability of resistance to temperature was improved. The repeated use number of the phenylated SBA-15 immobilized lipase as catalyst was improved compared with that of SBA-15 immobilized lipase.

Keywords

SBA-15 mesoporous material Lipase Catalytic performance Characterization
Sun, S.-J., & Zhai, Q.-Z. (2014). Characterization of (SBA-15)-Lipase Composite Material. Asian Journal of Chemistry, 26(10), 3064–3072. https://doi.org/10.14233/ajchem.2014.16678
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