Fluorescence Spectroscopy in Study of Human-like Collagen and Endotoxin Interactions

Pei Ma; Yuan-Yuan Yu; Dai-Di Fan

doi:10.14233/ajchem.2014.15872

Issue

Vol. 26 No. 11 (2014): Vol 26 Issue 11

Issue Published : May 25, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Fluorescence Spectroscopy in Study of Human-like Collagen and Endotoxin Interactions

https://doi.org/10.14233/ajchem.2014.15872

Pei Ma

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an 710069, Shaanxi Province, P.R. China ;Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi Province, P.R. China; These authors contributed equally to this work.

Yuan-Yuan Yu

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an 710069, Shaanxi Province, P.R. China; Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi Province, P.R. China; Shaanxi Xueqian Normal University, Xi'an 710100, Shaanxi Province, P.R. China;These authors contributed equally to this work.

Dai-Di Fan

Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, Taibai North Road 229, Xi'an 710069, Shaanxi Province, P.R. China; Shaanxi R & D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710069, Shaanxi Province, P.R. China

Corresponding Author(s) : Dai-Di Fan

fandaidi@nwu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11
Article Published : May 27, 2014

Abstract

Removal of endotoxins from recombinant proteins is a critical and challenging step in the preparation of intravenous therapeutics, because of their potent biological activities causing pyrogenic and shock reactions. However, the interaction between protein and endotoxin makes it more complicated to remove endotoxin from target protein. In this paper, the binding of endotoxin with human-like collagen was investigated by fluorescence spectroscopy and the effect of surfactants on the human-like collagen-endotoxin complex was studied. The fluorescence titration results revealed that endotoxin has a strong ability to quench the intrinsic fluorescence of human-like collagen through static quenching mechanism according to the Stern-Volmer equation. The binding constants and the corresponding thermodynamic parameters at different temperatures were calculated, which showed that the hydrophobic force played a major role in the interaction of endotoxin with human-like collagen. In addition, Triton X-100 was described to remove endotoxin from human-like collagen. All these results and theoretical data clarified that endotoxin could interact with human-like collagen to form the complex and Triton X-100 has proven to be effective in the process of endotoxin removal, which could be a useful guideline for further endotoxin removal from recombinant protein.

Keywords

Human-like collagen Endotoxin Fluorescence Surfactant Titron X-100

Ma, P., Yu, Y.-Y., & Fan, D.-D. (2014). Fluorescence Spectroscopy in Study of Human-like Collagen and Endotoxin Interactions. Asian Journal of Chemistry, 26(11), 3175–3179. https://doi.org/10.14233/ajchem.2014.15872

Download Citation

References

S.S. de Freitas, R.L. Machado, E.J. de Arruda, C.C. Santana and S.M.A. Bueno, J. Membr. Sci., 234, 67 (2004); doi:10.1016/j.memsci.2003.12.019.
F.B. Anspach, J. Biochem. Biophys. Methods, 49, 665 (2001); doi:10.1016/S0165-022X(01)00228-7.
S.I. Morse, Adv. Appl. Microbiol., 20, 9 (1976); doi:10.1016/S0065-2164(08)70106-0.
D. Fumarola, Cell. Immunol., 58, 216 (1981); doi:10.1016/0008-8749(81)90163-5.
D. Petsch and F.B. Anspach, J. Biotechnol., 76, 97 (2000); doi:10.1016/S0168-1656(99)00185-6.
K.C. Hou and R. Zaniewski, J. Pharm. Sci. Technol., 44, 204 (1990).
H.W. Zhao, M. Ge, Z.X. Zhang, W.F. Wang and G.Z. Wu, Spectrochim. Acta A, 65, 811 (2006); doi:10.1016/j.saa.2005.12.038.
F.Q. Cheng, Y.P. Wang, Z.P. Li and C. Dong, Spectrochim. Acta A, 65, 1144 (2006); doi:10.1016/j.saa.2006.01.024.
D.B. Cordes, S. Gamsey and B. Singaram, Angew. Chem. Int. Ed., 45, 3829 (2006); doi:10.1002/anie.200504390.
M.S. Matos, J. Hofkens and M.H. Gehlen, J. Fluoresc., 18, 821 (2008); doi:10.1007/s10895-007-0309-7.
D.D. Fan, M.R. Duan, Y. Mi, J.R. Song, J.F. Xi, D.W. Wang and G.Z. Wang, J. Chem. Ind. Eng. (China), 53, 752 (2002).
X.F. Hua, D.D. Fan, Y.E. Luo, X. Zhang, H.J. Shi, Y. Mi, X.X. Ma, L.A. Shang and G.F. Zhao, Chin. J. Chem. Eng., 14, 242 (2006); doi:10.1016/S1004-9541(06)60065-7.
Y. Zhai and F.Z. Cui, J. Cryst. Growth, 291, 202 (2006); doi:10.1016/j.jcrysgro.2006.03.006.
K. Hu, F.Z. Cui, Q. Lv, J. Ma, Q.L. Feng, L. Xu and D.D. Fan, J. Biomed. Mater. Res. A, 84A, 483 (2008); doi:10.1002/jbm.a.31440.
K. Hu, Q. Lv, F.Z. Cui, Q.L. Feng and X.D. Kong, J. Bioact. Compat. Polym., 21, 23 (2006); doi:10.1177/0883911506060455.
C.H. Zhu, D.D. Fan, X.X. Ma, W.J. Xue, Y.Y. Yu, Y.E. Luo, B.W. Liu and L. Chen, J. Bioact. Compat. Polym., 24, 560 (2009); doi:10.1177/0883911509349689.
Z. Yuan, M. Yu, J.H. Li, G.H. Hou and H.Y. Wang, Biomaterials, 26, 2741 (2005); doi:10.1016/j.biomaterials.2004.07.027.
F.C. Pearson, Pyrogens: Endotoxins, LAL-testing and Depyrogenation, Marcel Dekker, New York & Basel (1985).
J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum, New York, edn 2 (1999).
X.M. He and D.C. Carter, Nature, 358, 209 (1992); doi:10.1038/358209a0.
H.N. Hou, Z.D. Qi, Y.-W. OuYang, F.-L. Liao, Y. Zhang and Y. Liu, J. Pharm. Biomed. Anal., 47, 134 (2008); doi:10.1016/j.jpba.2007.12.029.
X.Z. Feng, Z. Lin, L.J. Yang, C. Wang and C.L. Bai, Talanta, 47, 1223 (1998); doi:10.1016/S0039-9140(98)00198-2.
A. Papadopoulou, R.J. Green and R.A. Frazier, J. Agric. Food Chem., 53, 158 (2005); doi:10.1021/jf048693g.
S.F. Sun, B. Zhou, H.N. Hou, Y. Liu and G.Y. Xiang, Int. J. Biol. Macromol., 39, 197 (2006); doi:10.1016/j.ijbiomac.2006.03.020.
P.D. Ross and S. Subramanian, Biochemistry, 20, 3096 (1981); doi:10.1021/bi00514a017.

References

S.S. de Freitas, R.L. Machado, E.J. de Arruda, C.C. Santana and S.M.A. Bueno, J. Membr. Sci., 234, 67 (2004); doi:10.1016/j.memsci.2003.12.019.

F.B. Anspach, J. Biochem. Biophys. Methods, 49, 665 (2001); doi:10.1016/S0165-022X(01)00228-7.

S.I. Morse, Adv. Appl. Microbiol., 20, 9 (1976); doi:10.1016/S0065-2164(08)70106-0.

D. Fumarola, Cell. Immunol., 58, 216 (1981); doi:10.1016/0008-8749(81)90163-5.

D. Petsch and F.B. Anspach, J. Biotechnol., 76, 97 (2000); doi:10.1016/S0168-1656(99)00185-6.

K.C. Hou and R. Zaniewski, J. Pharm. Sci. Technol., 44, 204 (1990).

H.W. Zhao, M. Ge, Z.X. Zhang, W.F. Wang and G.Z. Wu, Spectrochim. Acta A, 65, 811 (2006); doi:10.1016/j.saa.2005.12.038.

F.Q. Cheng, Y.P. Wang, Z.P. Li and C. Dong, Spectrochim. Acta A, 65, 1144 (2006); doi:10.1016/j.saa.2006.01.024.

D.B. Cordes, S. Gamsey and B. Singaram, Angew. Chem. Int. Ed., 45, 3829 (2006); doi:10.1002/anie.200504390.

M.S. Matos, J. Hofkens and M.H. Gehlen, J. Fluoresc., 18, 821 (2008); doi:10.1007/s10895-007-0309-7.

D.D. Fan, M.R. Duan, Y. Mi, J.R. Song, J.F. Xi, D.W. Wang and G.Z. Wang, J. Chem. Ind. Eng. (China), 53, 752 (2002).

X.F. Hua, D.D. Fan, Y.E. Luo, X. Zhang, H.J. Shi, Y. Mi, X.X. Ma, L.A. Shang and G.F. Zhao, Chin. J. Chem. Eng., 14, 242 (2006); doi:10.1016/S1004-9541(06)60065-7.

Y. Zhai and F.Z. Cui, J. Cryst. Growth, 291, 202 (2006); doi:10.1016/j.jcrysgro.2006.03.006.

K. Hu, F.Z. Cui, Q. Lv, J. Ma, Q.L. Feng, L. Xu and D.D. Fan, J. Biomed. Mater. Res. A, 84A, 483 (2008); doi:10.1002/jbm.a.31440.

K. Hu, Q. Lv, F.Z. Cui, Q.L. Feng and X.D. Kong, J. Bioact. Compat. Polym., 21, 23 (2006); doi:10.1177/0883911506060455.

C.H. Zhu, D.D. Fan, X.X. Ma, W.J. Xue, Y.Y. Yu, Y.E. Luo, B.W. Liu and L. Chen, J. Bioact. Compat. Polym., 24, 560 (2009); doi:10.1177/0883911509349689.

Z. Yuan, M. Yu, J.H. Li, G.H. Hou and H.Y. Wang, Biomaterials, 26, 2741 (2005); doi:10.1016/j.biomaterials.2004.07.027.

F.C. Pearson, Pyrogens: Endotoxins, LAL-testing and Depyrogenation, Marcel Dekker, New York & Basel (1985).

J.R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum, New York, edn 2 (1999).

X.M. He and D.C. Carter, Nature, 358, 209 (1992); doi:10.1038/358209a0.

H.N. Hou, Z.D. Qi, Y.-W. OuYang, F.-L. Liao, Y. Zhang and Y. Liu, J. Pharm. Biomed. Anal., 47, 134 (2008); doi:10.1016/j.jpba.2007.12.029.

X.Z. Feng, Z. Lin, L.J. Yang, C. Wang and C.L. Bai, Talanta, 47, 1223 (1998); doi:10.1016/S0039-9140(98)00198-2.

A. Papadopoulou, R.J. Green and R.A. Frazier, J. Agric. Food Chem., 53, 158 (2005); doi:10.1021/jf048693g.

S.F. Sun, B. Zhou, H.N. Hou, Y. Liu and G.Y. Xiang, Int. J. Biol. Macromol., 39, 197 (2006); doi:10.1016/j.ijbiomac.2006.03.020.

P.D. Ross and S. Subramanian, Biochemistry, 20, 3096 (1981); doi:10.1021/bi00514a017.

Issue

Vol. 26 No. 11 (2014): Vol 26 Issue 11

Fluorescence Spectroscopy in Study of Human-like Collagen and Endotoxin Interactions

Corresponding Author(s) : Dai-Di Fan

Abstract

Keywords

Full Article

Download Citation

References

Table Of Contents