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Vol. 26 No. 11 (2014): Vol 26 Issue 11

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Effect of Glutathione on Pyocyanin Production in Pseudomonas aeruginosa

https://doi.org/10.14233/ajchem.2014.17507
Yani Zhang
Faculty of Life Sciences, Northwest University, 229 Taibai Road, Xi'an 710069, Shaanxi Province, P.R. China
Xiaolong Shao
Faculty of Life Sciences, Northwest University, 229 Taibai Road, Xi'an 710069, Shaanxi Province, P.R. China
Shihong Wang
Faculty of Life Sciences, Northwest University, 229 Taibai Road, Xi'an 710069, Shaanxi Province, P.R. China
Siqi Zhang
Faculty of Life Sciences, Northwest University, 229 Taibai Road, Xi'an 710069, Shaanxi Province, P.R. China

Corresponding Author(s) : Yani Zhang

yani@nwu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11

Abstract

Pyocyanin secreted by Pseudomonas aeruginosa is a redox active virulence factor, generating superoxide and H2O2 in host cells. Glutathione (GSH) is the most effective antioxidant for removing these reactive oxygen species in cells. However, many pulmonary diseases such as cystic fibrosis are associated with the lower levels of glutathione in the epithelial lining fluid (ELF) than those in healthy individuals. Oxidative injury inflicted by P. aeruginosa is one of the major causes to aggravating cystic fibrosis disease. We hypothesized that glutathione plays an important role during P. aeruginosa mediated pathogenesis. To test this hypothesis, a glutathione-null gshB mutant was constructed. The result demonstrated that the gshB mutant has less pyocyanin than the wild type PAO1 (p < 0.01), while P. aeruginosa strains treated with glutathione increased pyocyanin levels. This demonstrated glutathione can activate pyocyanin production. These findings may bring new insights into the molecular pathogenesis of P. aeruginosa infections and lead to novel therapeutic intervention for inhibiting P. aeruginosa infections.

Keywords

Pseudomonas aeruginosa Glutathione Pyocyanin Cystic fibrosis
Zhang, Y., Shao, X., Wang, S., & Zhang, S. (2014). Effect of Glutathione on Pyocyanin Production in Pseudomonas aeruginosa. Asian Journal of Chemistry, 26(11), 3265–3269. https://doi.org/10.14233/ajchem.2014.17507
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