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Vol. 26 No. 11 (2014): Vol 26 Issue 11

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Simultaneous Determination of Nine Active Ingredients in Belladonna herb by HPLC

https://doi.org/10.14233/ajchem.2014.15838
Wei Huang
Zhejiang Medical College, No. 481, Binwen Road, Binjiang Dist. 310053, Hangzhou, Zhejiang Province, P.R. China
Jie Gao
Zhejiang Chinese Medical University, No. 548 Binwen Road Binjiang Dist. 310053, Hangzhou, Zhejiang Province, P.R. China
Weiming Cheng
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou, Zhejiang Province, P.R. China
Qinwei Huang
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou, Zhejiang Province, P.R. China
Lei Luo
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou, Zhejiang Province, P.R. China
Chao Yue
Zhejiang Chinese Medical University, No. 548 Binwen Road Binjiang Dist. 310053, Hangzhou, Zhejiang Province, P.R. China
Wenting Zhang
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou, Zhejiang Province, P.R. China

Corresponding Author(s) : Wenting Zhang

leozhwt@163.com

Asian Journal of Chemistry, Vol. 26 No. 11 (2014): Vol 26 Issue 11

Abstract

An HPLC method is established for the simultaneous determination of nine active ingredients in Belladonna herb (B. herb). HPLC conditions included Agilent Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of methanol and 0.05 % phosphoric acid for gradient elution. The flow rate was 1 mL/min and the column temperature was 30 °C. Nine active ingredients had good linear relationships: scopolinin the range of 11.08-498.96 ng (R2 = 1.000), chlorogenic acid in the range of 14.57-2039.52 ng (R2 = 1.000), flavonoid glycoside A in the range of 32.04-4485.60 ng (R2 = 0.9999), flavonoid glycoside B in the range of 53.00-1484.00 ng (R2 = 1.000), flavonoid glycoside C in the range of 99.84-4992.00 ng (R2 = 0.9999), flavonoid glycoside D in the range of 43.20-1484.00 ng (R2 = 1.000), umbelliferone in the range of 3.84-133.84 ng (R2 = 1.000), scopoletin in the range of 4.45-278.00 ng (R2 = 0.9999) and rutin in the range of 25.23-3531.64 ng (R2 = 1.000). Their average recoveries were 101.4 % (RSD = 2.51 %), 95.0 % (RSD = 2.63 %), 96.7 % (RSD = 2.04 %), 97.5 % (RSD = 1.89 %), 98.3 % (RSD = 1.87% ), 101.2 % (RSD = 2.09 %), 104.8 % (RSD = 1.57 %), 103.4 %( RSD = 2.00) and 103.3 % (RSD = 2.29 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the nine active ingredients and quality control of B. herb.

Keywords

HPLC Belladonnae herb Active ingredients
Huang, W., Gao, J., Cheng, W., Huang, Q., Luo, L., Yue, C., & Zhang, W. (2014). Simultaneous Determination of Nine Active Ingredients in Belladonna herb by HPLC. Asian Journal of Chemistry, 26(11), 3134–3138. https://doi.org/10.14233/ajchem.2014.15838
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References
  1. State Pharmacopeia Committee of China, Chinese Pharmacopoeia 2010, version part I, p. 355 (2010).
  2. K. Sun and L.M. Yang, World Clinical Drugs, 31, 182 (2010).
  3. S. Liu, W.J. Zang, Z.l. Li, Q. Sun and X.J. Yu, J. Xi’an Jiaotong Univ. (Med. Sci.), 26, 16 (2005).
  4. L. He, S.L. Yang, D.S. Wu, T. Cui, D. Wei and Z.T. Ding, China J. Chin. Mater. Med., 37, 811 (2012).
  5. S.Y. Zhang, L. Meng, W.Y. Gao, N.-N. Song, W. Jia and H.Q. Duan, China J. Chin. Mater. Med., 30, 410 (2005).
  6. X.H. Yang, J.H. Cui, A.W. Ding, J. Sichuan TCM, 24 , 17 (2006).
  7. A. Suzuki, N. Yamamoto, H. Jokura, M. Yamamoto, A. Fujii, I. Tokimitsu and I. Saito, J. Hypertens., 24, 1065 (2006); doi:10.1097/01.hjh.0000226196.67052.c0.
  8. T. Watanabe, Y. Arai, Y. Mitsui, T. Kusaura, W. Okawa, Y. Kajihara and I. Saito, Clin. Exp. Hypertens., 28, 439 (2006); doi:10.1080/10641960600798655.
  9. K.S. Wang, Plants, 26, 26 (2003).
  10. A. Lange and P. Toft, Ugeskr. Laeger, 152, 1096 (1990).
  11. U. Cikla, S. Turkmen, Y. Karaca, F.A. Ayaz, S. Turedi and A. Gunduz, Hum. Exp. Toxicol., 30, 1998 (2011); doi:10.1177/0960327111407225.
  12. F. Laffargue, C. Oudot, A. Constanty, A. Bedu and S. Ketterer-Martinon, Arch Pediatr., 18, 186 (2011); doi:10.1016/j.arcped.2010.11.006.
  13. E.P. Krenzelok, Clin. Toxicol., 48, 104 (2010); doi:10.3109/15563651003630672.
  14. J. Kupper and C. Reichert, Ther. Umsch., 66, 343 (2009).
  15. A. Mateo Montoya, N. Mavrakanas and J.S. Schutz, Eur. J. Ophthalmol., 19, 170 (2009).
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