Separation of Cellulose-Decomposing Strain

Abstract

In order to find mixed strains which are highly effective in decomposing straw cellulose to decompose straws so as to utilize resources optimally, this research starts from separating strains and takes the soil chronically covered by straws and other plants as the sample. Through repeatable enrichment cultivation, separation and purification, fourteen natural strain stubs which can grow on solid cultivation medium with carboxymethyl cellulose medium-Na (CMC-Na) as the only carbon source are gradually obtained. Then, through the degradation test of filter paper and the identification of congo red cellulose, ten fungi with strong adaptability and excellent effect of decomposing cellulose are screened, from which four combination ways are formed. A combination of mixed strains with the best effect to decompose cellulose is finally gained by comparing the CMC, FPA enzyme activity and the syngenesis property of the four combinations. Through identification, the two strains are, respectively Mucor sp. and Aspergillus sp.