GC-MS Analysis and in vitro Cytotoxicity and Antioxidant Studies on Leucas aspera

S.T. Parimaladevi; C. David Raj; V. Sudha; P. Brindha

doi:10.14233/ajchem.2014.17052

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Issue Published : June 5, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

GC-MS Analysis and in vitro Cytotoxicity and Antioxidant Studies on Leucas aspera

https://doi.org/10.14233/ajchem.2014.17052

S.T. Parimaladevi

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

C. David Raj

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

V. Sudha

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

P. Brindha

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

Corresponding Author(s) : P. Brindha

brindha@carism.sastra.edu

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12
Article Published : June 6, 2014

Abstract

Leucas aspera Spreng. is a common Laminaceous member whose leaf and its juice is traditionally used as medicine for various ailments. The aim of the present work is to prove the antioxidant and cytotoxic efficacy of various parts of Leucas aspera by in vitro methods. Leucas aspera commonly called “Thumbai” is collected from in and around Thanjavur. After proper identification and authentication the different parts of plant were separated and decoction prepared, filtered, lyophilized and subjected to in vitro and chemical studies. The preliminary phytochemical analysis of the test drug revealed the presence of sterols, flavonoids, terpenoids and reducing sugars. GC-MS analysis helped to identify the fragments of the chemical constituents present in the plant. In the present study, antioxidant and cytotoxic efficacy of the selected drug was established through in vitro methods. The efficacy confirmed is further supported by chemical evidences derived from GC-MS analysis.

Keywords

Leucas aspera GC-MS Antioxidant Cytotoxicity

Parimaladevi, S., David Raj, C., Sudha, V., & Brindha, P. (2014). GC-MS Analysis and in vitro Cytotoxicity and Antioxidant Studies on Leucas aspera. Asian Journal of Chemistry, 26(12), 3675–3678. https://doi.org/10.14233/ajchem.2014.17052

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References

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R. Vijay Kumar, G. Venkatrajireddy, T. Bikshapathi and M.K. Reddy, J. Photother. Pharmacol., 7, 1 (2012).

B. Shineyramya, P. Ganesh and R. Suresh Kumar, Int. J. Pharm. Biol. Arch., 3, 162 (2012).

P. Pushpangadan and C.K. Atal, J. Ethnopharmacol., 11, 59 (1984); doi:10.1016/0378-8741(84)90096-5.

T. Johns, G.M. Faubert, J.O. Kokwaro, R.L. Mahunnah and E.K. Kimanani, J. Ethnopharmacol., 46, 17 (1995); doi:10.1016/0378-8741(95)01224-2.

T. Johns, J.O. Kokwaro and E.K. Kimanani, Econ. Bot., 44, 369 (1990); doi:10.1007/BF03183922.

J.C. Saha, E.C. Savini and S. Kasinathan, Indian J. Med. Res., 49, 130 (1961).

N.K. Bhattarai, Tropical Doctor, 27, 43, (1997).

T.R. Sahu, Ancient Sci. Life, 3, 245 (1984).

W.A. Whistler, J. Ethnopharmacol., 13, 239 (1985); doi:10.1016/0378-8741(85)90072-8.

D.K. Holdsworth, Cool Islands, 29, 71 (1991).

G. Samuelsson, M.H. Farah, P. Claeson, M. Hagos, M. Thulin, O. Hedberg, A.M. Warfa, A.O. Hassan, A.H. Elmi, A.D. Abdurahman, A.S. Elmi, Y.A. Abdi and M.H. Alin, J. Ethnopharmacol., 37, 47 (1992); doi:10.1016/0378-8741(92)90004-B.

K.C. Tiwari, R. Majumder and S. Bhattacharjee, Pharm. Biol., 17, 61 (1979); doi:10.3109/13880207909067450.

J. Remington, in eds.: D.B. Troy and P. Beringer, In Remington: The Science and Practice of Pharmacy, Lippincott Williams and Wilkins, USA, edn 21. Volume I, pp. 510 (2006).

The Siddha Formulary of India, Govt. of India, Ministry of Health and Family Welfare, Department of Health, Volume-I, p. 159 (1992).

J.B. Harborne, Phytochemical Methods, A Guide to Modern Techniques of Plant Analysis, Chapman & Hall Pub London, U.K., edn 3 (1998).

A. Arnab, A.A. Goyal and S.S. Middha, J. Nat. Pharm., 1, 40 (2010); doi:10.4103/2229-5119.73586.

M. Oyaizu, Japan. J. Nutr., 44, 307 (1986).

T. Mosmann, J. Immunol. Methods, 65, 55 (1983); doi:10.1016/0022-1759(83)90303-4.

J. Xavier and N. Johnson, Int. J. Biol. Pharm. Allied Sci., 2, 894 (2013).

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O.I. Aruoma and S.L. Cuppett, Antioxidant Methodology in vivo and in vitro Concepts, AOCS Press, Champaign, Illinios, pp. 41-172 (1997)..

T. Osawa, Novel Natural Antioxidants For Utilization in Food and Biolo-gical Systems, Post-Harvest Biochemistry of Plant Food Materials in the Tropics, Japan Scientific Societies Press, Tokyo, Japan, pp. 241-251 (1994).

F.M. Benabdesselam, S. Khentache, K. Bougoffa, M. Chibane, S. Adach, Y. Chapeleur, H. Max and D. Laurain-Mattar, Rec. Nat. Prod., 1, 28 (2007).

H. Terasaka, S.R.M.D. Morshed, K. Hashimoto, H. Sakagami and S. Fujisawa, Anticancer Res., 25, 161 (2005).

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

GC-MS Analysis and in vitro Cytotoxicity and Antioxidant Studies on Leucas aspera

Corresponding Author(s) : P. Brindha

Abstract

Keywords

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