Extraction and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two Phase Extraction and its Applications

Senthilkumar Rathnasamy; J. Jennet Debora; B.N. Vedha Hari

doi:10.14233/ajchem.2014.17063

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Issue Published : June 5, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Extraction and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two Phase Extraction and its Applications

https://doi.org/10.14233/ajchem.2014.17063

Senthilkumar Rathnasamy

School of Chemical and Biotechnology, SASTRA University, Thanjavur-61 3401, India

J. Jennet Debora

School of Chemical and Biotechnology, SASTRA University, Thanjavur-61 3401, India

B.N. Vedha Hari

School of Chemical and Biotechnology, SASTRA University, Thanjavur-61 3401, India

Corresponding Author(s) : Senthilkumar Rathnasamy

rsenthilkumar@biotech.sastra.edu

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12
Article Published : June 6, 2014

Abstract

Spirulina platensis is a highly potential Cyanobacteria (blue green algae) that acts as immuno-modulator. Present study deals with the extraction of phycocyanin from this potent cyanobacteria employing non organic method. Organic denaturation and protein instability encountered in the conventional organic solvent extraction method can be overcome by biological extraction method. Phycocyanin, a phycobiliprotein present in the Spirulina extracted using aqueous two phase extraction and it was compared with isoelectric precipitation. The resulting fractions were spectrometrically assayed for phycocyanin content. The fraction with high phycocyanin content was identified and purified using anionic exchange chromatography. The resulting purified fraction was evaluated for antibacterial activity and antioxidant activity.

Keywords

Spirulina platensis Phycocyanin Aqueous two phase extraction Anionic purification antimicrobial activity

Rathnasamy, S., Jennet Debora, J., & Vedha Hari, B. (2014). Extraction and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two Phase Extraction and its Applications. Asian Journal of Chemistry, 26(12), 3729–3732. https://doi.org/10.14233/ajchem.2014.17063

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References

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References

F.S. Antelo, A. Anschau, J.A.V. Costa and S.J. Kalil, J. Braz. Chem. Soc., 21, 921 (2010); doi:10.1590/S0103-50532010000500022.

P. Prabuthas, S. Majumdar, P.P. Srivastav and H.N. Mishra, J. Stored Prod. Postharvest Res., 2, 93 (2011).

M. Rito-Palomares, J. Chromatogr. B, 807, 3 (2004); doi:10.1016/j.jchromb.2004.01.008.

G. Patil, S. Chethana, M.C. Madhusudhan and K.S.M.S. Raghavarao, Bioresour. Technol., 99, 7393 (2008); doi:10.1016/j.biortech.2008.01.028.

V. Kumar, A.K. Bhatnagar and J.N. Srivastava, J. Med. Plants Res., 5, 7043 (2011); doi:10.5897/JMPR11.1175.

C. Romay, R. Gonzalez, N. Ledon, D. Remirez and V. Rimbau, Curr. Protein Pept. Sci., 4, 207 (2003); doi:10.2174/1389203033487216.

S.K. Mishra, A. Shrivastav and S. Mishra, Process Biochem., 43, 339 (2008); doi:10.1016/j.procbio.2007.12.012.

Y. Zhu, X.B. Chen, K.B. Wang, Y.X. Li, K.Z. Bai, T.Y. Kuang and H.B. Ji, Appl. Microbiol. Biotechnol., 74, 244 (2007); doi:10.1007/s00253-006-0636-7.

J.-F. Niu, G.-C. Wang and C.-K. Tseng, Protein Expr. Purif., 49, 23 (2006); doi:10.1016/j.pep.2006.02.001.

R.B. Roman, J.M. Alvarez-Pez, F.G.A. Fernandez and E.M. Grima, J. Biotechnol., 93, 73 (2002); doi:10.1016/S0168-1656(01)00385-6.

M. Rito-Palomares, J. Chromatogr. B, 807, 3 (2004); doi:10.1016/j.jchromb.2004.01.008.

G. Patil, S. Chethana, M.C. Madhusudhan and K.S.M.S. Raghavarao, Bioresour. Technol., 99, 7393 (2008); doi:10.1016/j.biortech.2008.01.028.

A.K. Vinay Kumar, J. Med. Plants Res., 5, 7043 (2011); doi:10.5897/JMPR11.1175.

K.G. Sabarinathan, G. Ganesan, Eur. Rev. Med. Pharmacol. Sci., 12, 79 (2008).

Y. Zhu, X.B. Chen, K.B. Wang, Y.X. Li, K.Z. Bai, T.Y. Kuang and H.B. Ji, Appl. Microbiol. Biotechnol., 74, 244 (2007); doi:10.1007/s00253-006-0636-7.

A. Bennett and L. Bogorad, J. Cell Biol., 58, 419 (1973); doi:10.1083/jcb.58.2.419.

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Extraction and Purification of C-Phycocyanin from Spirulina platensis Using Aqueous Two Phase Extraction and its Applications

Corresponding Author(s) : Senthilkumar Rathnasamy

Abstract

Keywords

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