Standardization and in vitro Cytotoxic Studies on Narasimha lehiyam: A Potent Anticancer Siddha Drug

N. Ravichandran; P. Rajalakshmi; C. Davidraj; K.P. Arun; P. Brindha

doi:10.14233/ajchem.2014.17055

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Issue Published : June 5, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Standardization and in vitro Cytotoxic Studies on Narasimha lehiyam: A Potent Anticancer Siddha Drug

https://doi.org/10.14233/ajchem.2014.17055

N. Ravichandran

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

P. Rajalakshmi

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

C. Davidraj

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

K.P. Arun

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

P. Brindha

Centre for Advanced Research in Indian System of Medicine, SASTRA University, Thanjavur-613 401, India

Corresponding Author(s) : N. Ravichandran

brindha@carism.sastra.edu

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12
Article Published : June 6, 2014

Abstract

Cancer is one of the major killing disease, worldwide 10 million people were diagnosed every year with cancer. Chemical and radiation therapy are often recommemded treatments for cancer which often leads to a variety of side effects, hence herbal drugs are resolved to as an alternative therapy due its lesser side effects. But herbal drugs need standardization and validation studies. Narasimha lehiyam a herbal based Siddha formulation used to treat cancer, skin diseases, ulcer, leprosy, infectious diseases, glandular enlargement, dropsy, paralysis, diabetes, sinusitis, piles and pistula. The present paper was studied the standardization and validation point of view. Attempts were made to determine the quality parameters for this siddha formulation which include determination of organoleptic and physico chemical constants such as loss on drying, ash values and specific gravity. Besides total solid contents, fat contents, reducing sugars, alkaloids flavonoids, phenols, saponins and tannins were also estimated. The compounds present in the Narasimha lehiyam were identified using LC-MS/MS and GC-MS. The in vitro anticancer potential evaluated using MTT assay, the data of the results obtained were encouraging.

Keywords

Narasimha lehiyam Calcium oxalate crystals Resin Containing cells Micromorphology LC-MSMS and MTT assay

Ravichandran, N., Rajalakshmi, P., Davidraj, C., Arun, K., & Brindha, P. (2014). Standardization and in vitro Cytotoxic Studies on Narasimha lehiyam: A Potent Anticancer Siddha Drug. Asian Journal of Chemistry, 26(12), 3692–3696. https://doi.org/10.14233/ajchem.2014.17055

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References

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References

D.K. Ved and G.S. Goraya, Demand and Supply of Medicinal Plants in India In: M.P. Singh Publishers and Distributors of Scientific Books, pp. 108-115 (2008).

S.N. Mishra, Bhaisajya Ratnavali of Govida Das Sen, Chaukhaba Surbharati Prakasan; Varanasi, p. 780 (2007).

S. Panchawat, K.S. Rathore, S.S. Sisodia and R.K. Nema. Standardization and Evaluation of Herbal Drug Formulations (2010).

N.N. Satheesh Madhav, K. Upadhya and A. Bisht, Indian J. Physiol. Pharmacol., S3, 235 (2011).

WHO, The International Pharmacopeia, Quality Specifications for Pharmaceutical Substances, Excipients, and Dosage forms, World Health Organization, Geneva, edn 3 (1988).

S.K.M. Siddha, Ayurveda Company (India) Ltd. Therapeutic Index, SKM Siddha, Erode, Tamil Nadu, India, edn 12, p. 67 (2010).

S. Veeraperumal Pillai, Narasimha lehiyam In: Nam Nattu vaithiyam (Tamil) Veeraperumal Pillai Ed. Shanmuganadha Book Depot, Chennai, India (2006).

K.R. Khandelwal, Practical Pharmacognosy, Techniques and Experiments, Nirali Prakashan, Pune, pp. 149-156 (2002).

Anonymous, Parameters for Quality Assessment of Ayurveda and Siddha Drugs, Part A CCRAS, Department of AYUSH, Govt of India, New Delhi, pp. 8-9.

J.B. Harborne, Phytochemical Methods, Chapman and Hall, London, p. 113 (1973).

G.L. Miller, Anal. Chem., 31, 426 (1959); doi:10.1021/ac60147a030.

B.A. Boham and A.C. Kocipai, Pac. Sci., 48, 458 (1994).

E.O. Edeoga, D.E. Okwu and B.O. Mbaebi, Afr. J. Biotechnol., 4, 685 (2005).

T. Mosmann, J. Immunol. Methods, 65, 55 (1983); doi:10.1016/0022-1759(83)90303-4.

G.C. Jagetia and S.K. Rao, Biol. Pharm. Bull., 29, 460 (2006); doi:10.1248/bpb.29.460.

S. Rahman, F. Salehin and A. Iqbal, BMC Complement. Altern. Med., 11, 76 (2011); doi:10.1186/1472-6882-11-76.

P.U. Devi, F.E. Solomon and A.C. Sharada, Indian J. Exp. Biol., 32, 523 (1994).

K.R. Kirtikar and B.D. Basu, Indian Medicinal Plants, International Book Distributors, Deharadun, edn 4 (1996).

P. Mathivadhani, P. Shanthi and P. Sachdanandam, Cell Biol. Int., 31, 1198 (2007); doi:10.1016/j.cellbi.2007.04.004.

N.S. Prakash, R. Sundaram and S.K. Mitra, Indian J. Pharmacol., 44, 732 (2012); doi:10.4103/0253-7613.103273.

H.J. Kim, J.C. Kim, J.S. Min, M.J. Kim, J.A. Kim, M.H. Kor, H.S. Yoo and J.K. Ahn, J. Ethnopharmacol., 136, 197 (2011); doi:10.1016/j.jep.2011.04.060.

J. Tavakkol-Afshari, A. Brook and S.H. Mousavi, Food Chem. Toxicol., 46, 3443 (2008); doi:10.1016/j.fct.2008.08.018.

L.W. Bieber, Á.A. Da Silva Filho, R.M.O. Corréa Lima, A. De Andrade Chiappeta, S.C. Do Nascimento, I.A. De Souza, J.F. De Méllo and H.J. Veith, Phytochemistry, 25, 1077 (1986); doi:10.1016/S0031-9422(00)81557-5.

K. Selvendiran, J.P. Singh, K.B. Krishnan and D. Sakthisekaran, Fitoterapia, 74, 109 (2003); doi:10.1016/S0367-326X(02)00304-0.

E.S. Sunila and G. Kuttan, J. Ethnopharmacol., 90, 339 (2004); doi:10.1016/j.jep.2003.10.016.

Y.M. Lo, Food Sci. Nutr., 1, 1 (2013); doi:10.1002/fsn3.3.

S. Banerjee and C. Kr, Carcinogenesis, 27, 1645 (2005); doi:10.1093/carcin/bgi372.

S.G. Rathi, M. Suthar, P. Patel, V.H. Bhaskar and N.B. Rajgor, J. Young Pharm., 1, 239 (2009); doi:10.4103/0975-1483.57071.

A. Singh, S. Dugga, N. Kaur and J. Singh, J. Nat. Prod., 3, 64 (2010).

B. Bhattacharjee and J. Chatterjee, Asian Pac. J. Cancer Prev., 14, 3735 (2013); doi:10.7314/APJCP.2013.14.6.3735.

P. Balachandran and R. Govindarajan, Pharm. Res., 51, 19 (2005); doi:10.1016/j.phrs.2004.04.010.

Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Standardization and in vitro Cytotoxic Studies on Narasimha lehiyam: A Potent Anticancer Siddha Drug

Corresponding Author(s) : N. Ravichandran

Abstract

Keywords

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