Issue

Vol. 26 No. 12 (2014): Vol 26 Issue 12

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Preparation and Characterization of Long Alkyl Chain Methacrylate-Based Monolithic Column for Capillary Chromatography in Separation of Alkylbenzene Compound

https://doi.org/10.14233/ajchem.2014.16536
Athika Rahmah
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas Universiy, Padang 25163, Indonesia
Fitri Mairizki
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas Universiy, Padang 25163, Indonesia
Radhia Putri
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas Universiy, Padang 25163, Indonesia
Rahmiana Zein
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas Universiy, Padang 25163, Indonesia
Edison Munaf
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas Universiy, Padang 25163, Indonesia
Toyohide Takeuchi
Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan
Lee Wah Lim
Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan

Corresponding Author(s) : Edison Munaf

emunaf@fmipa.unand.ac.id

Asian Journal of Chemistry, Vol. 26 No. 12 (2014): Vol 26 Issue 12

Abstract

This research describes preparation and characterization of long alkyl chain methacrylate-base monolith for using as stationary phase in capillary liquid chromatography. Preparation of monolithic column were used dodecylmethacrylate as monomer, ethylene dimethacrylate as crosslinker and used a binary porogenic solvent consist of 1.4-butanediol and other 4 various porogen: ethanol, 1-propanol, isoamyl alcohol and isobutyl alcohol. The monolith column were prepared in 100 × 0.32 mm i.d. capillaries column and were optimized in effect of various mobile phase and effect of concentration of mobile phase for separation alkyl benzene compound. Morphology of monolithic column were characterized using scanning electron microscopy. Permeability of column (Ko) was measured by pumping water through to the column with different linear flow rate. The resulting monolithic column with 1.4-butanediol and 1-propanol as porogen have good performed in separation of alkyl benzene compound with acetonitrile-water (80:20) as mobile phase.

Keywords

Monolithic column Capillary chromatography Alkyl benzene
Rahmah, A., Mairizki, F., Putri, R., Zein, R., Munaf, E., Takeuchi, T., & Wah Lim, L. (2014). Preparation and Characterization of Long Alkyl Chain Methacrylate-Based Monolithic Column for Capillary Chromatography in Separation of Alkylbenzene Compound. Asian Journal of Chemistry, 26(12), 3595–3599. https://doi.org/10.14233/ajchem.2014.16536
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Z. Jiang, N.W. Smith, P. David Ferguson and M. Robert Taylor, J. Biochem. Biophys. Methods, 70, 39 ( (2007); doi:10.1016/j.jbbm.2006.08.009.
  2. Y. Li, H.D. Tolley and M.L. Lee, J. Chromatogr. A, 1218, 1399 (2011); doi:10.1016/j.chroma.2011.01.028.
  3. R.D. Arrua, M. Talebi, T.J. Causon and E.F. Hilder, Anal. Chim. Acta, 738, 1 (2012); doi:10.1016/j.aca.2012.05.052.
  4. G. Guiochon, J. Chromatogr. A, 1168, 101 (2007); doi:10.1016/j.chroma.2007.05.090.
  5. A. Suzuki, L.W. Lim, T. Hiroi and T. Takeuchi, Talanta, 70, 190 (2006); doi:10.1016/j.talanta.2005.12.064.
  6. Z.A. ALOthman, A. Aqel, H.A. Al Abdelmoneim, A. Yacine Badjah-Hadj-Ahmed and A.A. Al-Warthan, Chromatographia, 74, 1 (2011); doi:10.1007/s10337-011-2047-x.
  7. Y. Li, H. Dennis Tolley and M.L. Lee, J. Chromatogr. A, 1217, 4934 (2010); doi:10.1016/j.chroma.2010.05.048.
  8. Y. Li, M.L. Lee, J. Jin and J. Chen, Talanta, 99, 91 (2012); doi:10.1016/j.talanta.2012.04.066.
  9. S.H. Lubbad and M.R. Buchmeiser, J. Chromatogr. A, 1217, 3223 (2010); doi:10.1016/j.chroma.2009.10.090.
  10. G. Zhu, L. Zhang, H. Yuan, Z. Liang, W. Zhang and Y. Zhang, J. Sep. Sci., 30, 792 (2007); doi:10.1002/jssc.200600496.
  11. P. Coufal, M. Cihak, J. Suchankova, E. Tesarova, Z. Bosakova and K. Stulik, J. Chromatogr. A, 946, 99 (2002); doi:10.1016/S0021-9673(01)01570-9.
  12. M.L. Chen, L.M. Li, B.F. Yuan, Q. Ma and Y.-Q. Feng, J. Chromatogr. A, 1230, 54 (2012); doi:10.1016/j.chroma.2012.01.065.
  13. K. Liu, H.D. Tolley and M.L. Lee, J. Chromatogr. A, 1227, 96 (2012); doi:10.1016/j.chroma.2011.12.081.
  14. M. Takahashi, T. Hirano, S. Kitagawa and H. Ohtani, J. Chromatogr. A, 1232, 123 (2012); doi:10.1016/j.chroma.2011.10.070.
  15. J. Ou, G.T.T. Gibson and R.D. Oleschuk, J. Chromatogr. A, 1217, 3628 (2010); doi:10.1016/j.chroma.2010.03.043.
  16. K. Štulík, V. Pacáková, J. Suchánková and P. Coufal, J. Chromatogr. B, 841, 79 (2006); doi:10.1016/j.jchromb.2006.04.016.
  17. J. Urban and P. Jandera, J. Sep. Sci., 31, 2521 (2008); doi:10.1002/jssc.200800182.
  18. J. Vidic, A. Podgornik and A. Strancar, J. Chromatogr. A, 1065, 51 (2005); doi:10.1016/j.chroma.2004.11.044.
  19. G.T.T. Gibson, S.M. Mugo and R.D. Oleschuk, Polymer, 49, 3084 (2008); doi:10.1016/j.polymer.2008.05.014.
  20. E.G. Vlakh and T.B. Tennikova, J. Sep. Sci., 30, 2801 (2007); doi:10.1002/jssc.200700284.
  21. P. Jandera, M. Stankova, V. Skerikova and J. Urban, J. Chromatogr. A, 1274, 97 (2013); doi:10.1016/j.chroma.2012.12.003.
  22. T. Ikegami and N. Tanaka, Curr. Opin. Chem. Biol., 8, 527 (2004); doi:10.1016/j.cbpa.2004.08.009.
  23. Z. Xu, L. Yang and Q. Wang, J. Chromatogr. A, 1216, 3098 (2009); doi:10.1016/j.chroma.2009.01.089.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0