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Vol. 26 No. 13 (2014): Vol 26 Issue 13

Copyright (c) 2014 AJC

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Development of 4-Chloro-3,5-dinitrobenzotrifluoride Derivatization and Ultrahigh-performance Liquid Chromatography Method for Determination of 20 Free Amino Acids in Mature Vinegar

https://doi.org/10.14233/ajchem.2014.16012
Qin-Bao Lin
Key Laboratory of Product Packaging and Logistics of Guangdong Higher Education Institutes, Packaging Engineering Institute, Jinan University, Zhuhai 519070, P.R. China; Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, P.R. China
Lan-Lan Che
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, P.R. China
Jie Guo
Shanxi Academy of Analytical Science, Taiyuan 030006, P.R. China
Long-Fei Cai
Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, P.R. China
Rong-Zhen Wang
Shanxi Academy of Analytical Science, Taiyuan 030006, P.R. China

Corresponding Author(s) : Qin-Bao Lin

tlinqb@jnu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 13 (2014): Vol 26 Issue 13

Abstract

A rapid ultrahigh-performance liquid chromatography method has been established for the simultaneous determination of 20 free amino acids in mature vinegar. The chromatographic conditions, pretreatment methods and matrix effects were optimized. 4-Chloro-3,5-dinitrobenzotrifluoride was selected as a precolumn derivatizing reagent and can readily react with both primary and secondary amines. The separation of 20 amino acids was achieved within 15 min by gradient elution mode. The detection limits for the 20 different amino acids studied ranged from 1 to 9 mg L-1. The correlation coefficient values (r2 = 0.9945) indicated good correlations between the concentrations of the compounds under investigation and their peak areas within the responding ranges. Samples spiked at 50, 80 and 100 mg L-1 showed recoveries ranging from 70.1 to 109.2 % (except for tryptophan spiked with 50 mg L-1, which showed a recovery of 59.3 %) with relative standard deviations below 5.6 %. The developed method was rapid and showed high levels of efficiency, accuracy and reproducibility that could be used effectively for the simultaneous determination of 20 free amino acids in mature vinegar.

Keywords

Amino Acids Ultra high performance liquid chromatography Mature vinegar 4-Chloro-3,5-dinitrobenzotrifluoride
Lin, Q.-B., Che, L.-L., Guo, J., Cai, L.-F., & Wang, R.-Z. (2014). Development of 4-Chloro-3,5-dinitrobenzotrifluoride Derivatization and Ultrahigh-performance Liquid Chromatography Method for Determination of 20 Free Amino Acids in Mature Vinegar. Asian Journal of Chemistry, 26(13), 3909–3916. https://doi.org/10.14233/ajchem.2014.16012
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