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Vol. 26 No. 13 (2014): Vol 26 Issue 13

Copyright (c) 2014 AJC

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Chemical Fingerprinting of Tussilago farfara by RP-HPLC and Hierarchical Clustering Analysis

https://doi.org/10.14233/ajchem.2014.16550
Chang-Tian Li
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, P.R. China;These authors made equal contributions to this work
Zheng-Fei Yan
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, P.R. China; These authors made equal contributions to this work
Feng-Hua Tian
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, P.R. China ;College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, P.R. China;These authors made equal contributions to this work
Xin-Xin Mao
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, P.R. China
Yu Li
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, P.R. China

Corresponding Author(s) : Yu Li

lct@jlau.edu.cn; 441507293@qq.com

Asian Journal of Chemistry, Vol. 26 No. 13 (2014): Vol 26 Issue 13

Abstract

The aim of this study was to establish a method for extraction and chemically fingerprinting of extracts of Tussilago farfara and to apply this method to 12 T. farfara samples collected from different regions in China. RP-HPLC with gradient elution was performed on rutin. Comparing with the chromatograms, it showed that the samples were divided into two groups. Thirteen characteristic peaks were found in the extracts of all samples. Peak at retention time of 17.45 min was identified as rutin. Hierarchical clustering analysis showed the similar results as the visual comparison. The RP-HPLC method allowed it simple to identify and compare T. farfara. This was the first report on hierarchical clustering analysis of T. farfara.

Keywords

Chemical fingerprinting Tussilago farfara Hierarchical clustering analysis Characteristic peaks
Li, C.-T., Yan, Z.-F., Tian, F.-H., Mao, X.-X., & Li, Y. (2014). Chemical Fingerprinting of Tussilago farfara by RP-HPLC and Hierarchical Clustering Analysis. Asian Journal of Chemistry, 26(13), 4003–4006. https://doi.org/10.14233/ajchem.2014.16550
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