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Vol. 26 No. 14 (2014): Vol 26 Issue 14

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Comparative Extraction and Downstream Processing Techniques for Quantitative Analysis of Rosmarinic Acid in Rosmarinus officinalis

https://doi.org/10.14233/ajchem.2014.16266
Arnab Chatterjee
Process Chemistry and Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226 015, India
Sudeep Tandon
Process Chemistry and Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226 015, India
Ateeque Ahmad
Process Chemistry and Technology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow-226 015, India

Corresponding Author(s) : Ateeque Ahmad

ateeque97@gmail.com

Asian Journal of Chemistry, Vol. 26 No. 14 (2014): Vol 26 Issue 14

Abstract

Evaluation of different extraction and downstream processing techniques for the optimized extraction and quantification of rosmarinic acid (RA) in rosemary leaves has been reported. Quantitative analysis was accomplished on a Waters symmetry C18 (150 × 3.9 mm i.d., 5 μm) column using 0.1 % formic acid in water and methanol as mobile phase under linear gradient elution at 330 nm. Method represented a greater degree of linearity (r2 = 0.99) and precision with satisfactory recoveries (98.35-101.33 %). Extractive process development showed alcohol/water (7:3) as the optimum combining ratio during solvent optimization parameters in terms of extraction efficiency at ambient conditions (14.17-19.83 μg g-1). Ultrasonic-assisted extraction showed a two fold enhancement in the content of rosmarinic acid (45.55 μg g-1) for 70 % aqueous methanol whereas a three fold enhancement (48.09 μg g-1) was observed for microwave-assisted extraction in 70 % aqueous ethanol combination. Novelty of the downstream enrichment technique uses the adoption of a solid-matrix partitioning technique which subtantially enhanced the content of rosmarinic acid during sequential solvent partitioning.

Keywords

Rosmarinus officinalis Energy assisted extraction techniques Rosmarinic acid Solid matrix partitioning technique
Chatterjee, A., Tandon, S., & Ahmad, A. (2014). Comparative Extraction and Downstream Processing Techniques for Quantitative Analysis of Rosmarinic Acid in Rosmarinus officinalis. Asian Journal of Chemistry, 26(14), 4313–4318. https://doi.org/10.14233/ajchem.2014.16266
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References
  1. K. Schwarz and W. Ternes, Z. Lebensm. Unters. Forsch., 195, 95 (1992); doi:10.1007/BF01201765.
  2. O.I. Aruoma, B. Halliwell, R. Aeschbach and J. Loligers, Xenobiotica, 22, 257 (1992); doi:10.3109/00498259209046624.
  3. E.N. Frankel, S. Huang, R. Aeschbach and E. Prior, J. Agric. Food Chem., 44, 131 (1996); doi:10.1021/jf950374p.
  4. W. Zheng and S.Y. Wang, J. Agric. Food Chem., 49, 5165 (2001); doi:10.1021/jf010697n.
  5. M.R. Al-Sereiti, K.M. Abu-Amer and P. Sen, Indian J. Exp. Biol., 37, 124 (1999).
  6. H. Ito, T. Miyazaki, M. Ono and H. Sakurai, Bioorg. Med. Chem., 6, 1051 (1998); doi:10.1016/S0968-0896(98)00063-7.
  7. H. Takeda, M. Tsuji, T. Matsumiya and M. Kubo, Nihon Shinkei Yakurigaku Zasshi, 22, 15 (2002).
  8. R.C. Wren, Potter’s New Encyclopedia of Botanical Drugs and Preparations, The CW Daniel Company Ltd., (England) (1988).
  9. M.L. Scarpati and G. Oriente, Ricerca Sci., 28, 2392 (1958).
  10. K. Triantaphyllou, G. Blekas and D. Boskou, Int. J. Food Sci. Nutr., 52, 313 (2001); doi:10.1080/09637480120057512.
  11. M.H. Yu, J.H. Choi, I.G. Chae, H.G. Im, S.A. Yang, K. More, I.S. Lee and J. Lee, Food Chem., 136, 1047 (2013); doi:10.1016/j.foodchem.2012.08.085.
  12. M. Petersen and M.S.J. Simmonds, Phytochemistry, 62, 121 (2003); doi:10.1016/S0031-9422(02)00513-7.
  13. M.J. del Bano, J. Lotente, J. Castillo, O. Benavente-García, J.A. del Río, A. Ortuño, K.W. Quirin and D. Gerard, J. Agric. Food. Chem., 51, 4247 (2003); doi:10.1021/jf0300745.
  14. A. Sanchez-Medina, C.A. Etheridge, G.E. Hawkes, P.J. Hylands, B.A. Pendry, M.J. Hughes and O. Corcoran, J. Pharm. Sci.-US, 10, 455 (2007).
  15. H. Wang, G.J. Provon and K. Heliwell, Food Chem., 87, 307 (2004); doi:10.1016/j.foodchem.2003.12.029.
  16. M.L. Scarpati and G. Oriente, Tetrahedron, 4, 43 (1958); doi:10.1016/0040-4020(58)88005-9.
  17. Gestirner and Schiemenz, Sci. Pharm., 37, 40 (1969).
  18. B. Christ and K. Kurt, US Patent 4,354,0345 (1982).
  19. M. Herrero, M. Plaza, A. Cifuentes and E. Ibáñez, J. Chromatogr. A, 1217, 2512 (2010); doi:10.1016/j.chroma.2009.11.032.
  20. ICH (Q2), Proceedings of the International Conference on Harmonization, IFPMA, Geneva (2005).
  21. M. Thompson, SL.R. Ellison and R. Wood, Pure Appl. Chem., 74, 835 (2002); doi:10.1351/pac200274050835.
  22. R.G.D. Steele and J.H. Torrie, Principles and Procedures of Statistics, McGraw Hill, New York (1980).
  23. ICH Harmonized Tripartite Guideline: Validation of Analytical Procedures: Text and Methodology Q2 (R1), October, Step 4 version (1994).
  24. S. Tandon, A. Chatterjee, S.K. Chattopadhyay, R. Kaur and A.K. Gupta, Ind. Crops Prod., 31, 335 (2010); doi:10.1016/j.indcrop.2009.11.014
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