Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins

Y.Q. Cao; N. Wei; A.J. Gong; L.N. Qiu

doi:10.14233/ajchem.2014.16069

Issue

Vol. 26 No. 14 (2014): Vol 26 Issue 14

Issue Published : July 5, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins

https://doi.org/10.14233/ajchem.2014.16069

Y.Q. Cao

School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

N. Wei

School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

A.J. Gong

School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

L.N. Qiu

School of Chemistry and Biology Engineering, University of Science and Technology Beijing, Beijing 100083, P.R. China

Corresponding Author(s) : A.J. Gong

gongaijun5661@ustb.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 14 (2014): Vol 26 Issue 14
Article Published : July 5, 2014

Abstract

An effective method for large-scale extraction and purification of microcystin-LR (MC-LR) was established. 660 mL supernatant centrifugated from algae serum was absorbed by resin D101. The impurities on D101 resin were removed by 40 % acetic acid (3 L), deionized water (2.5 L), 20 % acetone (2 L) and 10 % ethanol (2 L) in turn. Thus microcystin-LR on D101 resin was purified. The adsorbed microcystin-LR was eluted from the resin with 70 % methanol (9 L). The eluent was concentrated by rotary evaporation at 80 °C. The quality of microcystin-LR in the crude extract was 2075.5 μg. The recovery rate was 79.71 %. In addition, the method of getting microcystin-LR by preparative chromatography is established. The purity of the product was 95 %.

Keywords

Microcystin-LR Macroporous resin Extraction Purification

Cao, Y., Wei, N., Gong, A., & Qiu, L. (2014). Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins. Asian Journal of Chemistry, 26(14), 4206–4210. https://doi.org/10.14233/ajchem.2014.16069

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References

L. Spoof, M.R. Neffling and J. Meriluoto, J. Chromatogr. B, 877, 3822 (2009); doi:10.1016/j.jchromb.2009.09.028.
D. Gutiérrez-Praena, Á. Jos, S. Pichardo, I.M. Moreno and A.M. Cameán, Food Chem. Toxicol., 53, 139 (2013); doi:10.1016/j.fct.2012.10.062.
V.R. Beasley, R.A. Lovell, and K.R. Holmes. Val R. Beasley, Randall A. Lovell, J. Toxicol. Environ. Health A, 61, 281 (2000); doi:10.1080/00984100050136599.
A.R. Humpage and S.J. Hardy, J. Toxicol. Environ. Health A, 61, 155 (2000); doi:10.1080/00984100050131305.
World Health Organization, WHO Guidelines for Drinking-water Quality Recommendations Chemical Fact, Geneva, Switzerland, edn 3 (2004).
I. Chorus and J. Bartram, Toxic Cyanobacteria in Water: A Guide to their Public Health Consequences, Monitoring and Management, E & FN Spon, London, p. 416 (1999).
WHO, Guidelines for Drinking Water Quality, Geneva, Switzerland (1998).
J. Chen, P. Xie, L. Li and J. Xu, Toxicol. Sci., 108, 81 (2009); doi:10.1093/toxsci/kfp009.
N.W. Carmichael, Sci. Am., 270, 78 (1994); doi:10.1038/scientificamerican0194-78.
J.A. Zweigenbaum, J.D. Henion, K.A. Beattie, G.A. Codd and G.K. Poon, J. Pharm. Biomed. Anal., 23, 723 (2000); doi:10.1016/S0731-7085(00)00354-X.
Y. Polyak, T. Zaytseva and N. Medvedeva, Water Air Soil Pollut., 224, 1494 (2013); doi:10.1007/s11270-013-1494-4.
J.E. Eriksson, D. Toivola, J.A.O. Meriluoto, H. Karaki, Y.G. Han and D. Hartshorne, Biochem. Biophys. Res. Commun., 173, 1347 (1990); doi:10.1016/S0006-291X(05)80936-2.
M. Runnegar, D.J. Seward, N. Ballatori, J.M. Crawforde and J.L. Boyer, Toxicol. Appl. Pharmacol., 161, 40 (1999); doi:10.1006/taap.1999.8783.
M. Thirumavalavan, Y.L. Hu and J.F. Lee, Toxicol. Environ. Chem., 95, 221 (2013); doi:10.1080/02772248.2013.768368.
W.Y. Zhang, N. Han, L.R. Yao, X.L. Qiu and X.L. Chen, Adv. Mater. Res., 340, 318 (2011); doi:10.4028/www.scientific.net/AMR.340.318.
L.J. Zhang, L.H. Yin, Y.P. Pu and G.C. Zhu, J. Southeast Univ. (Nat. Sci. Ed.), 3, 446 (2005).
X. Wu, C. Wang, B. Xiao, Y. Wang, N. Zheng and J. Liu, Anal. Chim. Acta, 709, 66 (2012); doi:10.1016/j.aca.2011.10.027.
D. Pyo and C. Lim, J. Liq. Chromatogr. Rel. Technol., 29, 2691 (2006); doi:10.1080/10826070600923233.
J.L. Wang, W.Y. Liang and L. Chen, J. Beijing Forestry Univ., 2, 184 (2010).
T. Wei, Z. Xiang X.G. Feng and J.J. Zhang, Jiangsu Environ. Sci. Technol., 5, 001 (2007).
W.W Zou, A.J. Gong, Y.Q. Cao, M.N. Liu and H.T. Yuan, Chem. Bioeng., 8. 025 (2008).
L.L. Liang, A.J. Gong, H.M. Li, Y.Q. Cao and B.Q. Li, Chin. J. Anal. Chem., 5, 040 (2010).
H.V. Frias, M.A. Mendes, K.H.M. Cardozo, V.M. Carvalho, D. Tomazela, P. Colepicolo and E. Pinto, Biochem. Biophys. Res. Commun., 344, 741 (2006); doi:10.1016/j.bbrc.2006.03.199.

References

L. Spoof, M.R. Neffling and J. Meriluoto, J. Chromatogr. B, 877, 3822 (2009); doi:10.1016/j.jchromb.2009.09.028.

D. Gutiérrez-Praena, Á. Jos, S. Pichardo, I.M. Moreno and A.M. Cameán, Food Chem. Toxicol., 53, 139 (2013); doi:10.1016/j.fct.2012.10.062.

V.R. Beasley, R.A. Lovell, and K.R. Holmes. Val R. Beasley, Randall A. Lovell, J. Toxicol. Environ. Health A, 61, 281 (2000); doi:10.1080/00984100050136599.

A.R. Humpage and S.J. Hardy, J. Toxicol. Environ. Health A, 61, 155 (2000); doi:10.1080/00984100050131305.

World Health Organization, WHO Guidelines for Drinking-water Quality Recommendations Chemical Fact, Geneva, Switzerland, edn 3 (2004).

I. Chorus and J. Bartram, Toxic Cyanobacteria in Water: A Guide to their Public Health Consequences, Monitoring and Management, E & FN Spon, London, p. 416 (1999).

WHO, Guidelines for Drinking Water Quality, Geneva, Switzerland (1998).

J. Chen, P. Xie, L. Li and J. Xu, Toxicol. Sci., 108, 81 (2009); doi:10.1093/toxsci/kfp009.

N.W. Carmichael, Sci. Am., 270, 78 (1994); doi:10.1038/scientificamerican0194-78.

J.A. Zweigenbaum, J.D. Henion, K.A. Beattie, G.A. Codd and G.K. Poon, J. Pharm. Biomed. Anal., 23, 723 (2000); doi:10.1016/S0731-7085(00)00354-X.

Y. Polyak, T. Zaytseva and N. Medvedeva, Water Air Soil Pollut., 224, 1494 (2013); doi:10.1007/s11270-013-1494-4.

J.E. Eriksson, D. Toivola, J.A.O. Meriluoto, H. Karaki, Y.G. Han and D. Hartshorne, Biochem. Biophys. Res. Commun., 173, 1347 (1990); doi:10.1016/S0006-291X(05)80936-2.

M. Runnegar, D.J. Seward, N. Ballatori, J.M. Crawforde and J.L. Boyer, Toxicol. Appl. Pharmacol., 161, 40 (1999); doi:10.1006/taap.1999.8783.

M. Thirumavalavan, Y.L. Hu and J.F. Lee, Toxicol. Environ. Chem., 95, 221 (2013); doi:10.1080/02772248.2013.768368.

W.Y. Zhang, N. Han, L.R. Yao, X.L. Qiu and X.L. Chen, Adv. Mater. Res., 340, 318 (2011); doi:10.4028/www.scientific.net/AMR.340.318.

L.J. Zhang, L.H. Yin, Y.P. Pu and G.C. Zhu, J. Southeast Univ. (Nat. Sci. Ed.), 3, 446 (2005).

X. Wu, C. Wang, B. Xiao, Y. Wang, N. Zheng and J. Liu, Anal. Chim. Acta, 709, 66 (2012); doi:10.1016/j.aca.2011.10.027.

D. Pyo and C. Lim, J. Liq. Chromatogr. Rel. Technol., 29, 2691 (2006); doi:10.1080/10826070600923233.

J.L. Wang, W.Y. Liang and L. Chen, J. Beijing Forestry Univ., 2, 184 (2010).

T. Wei, Z. Xiang X.G. Feng and J.J. Zhang, Jiangsu Environ. Sci. Technol., 5, 001 (2007).

W.W Zou, A.J. Gong, Y.Q. Cao, M.N. Liu and H.T. Yuan, Chem. Bioeng., 8. 025 (2008).

L.L. Liang, A.J. Gong, H.M. Li, Y.Q. Cao and B.Q. Li, Chin. J. Anal. Chem., 5, 040 (2010).

H.V. Frias, M.A. Mendes, K.H.M. Cardozo, V.M. Carvalho, D. Tomazela, P. Colepicolo and E. Pinto, Biochem. Biophys. Res. Commun., 344, 741 (2006); doi:10.1016/j.bbrc.2006.03.199.

Issue

Vol. 26 No. 14 (2014): Vol 26 Issue 14

Large-Scale Extraction and Purification of Microcystin-LR by Macroporous Resins

Corresponding Author(s) : A.J. Gong

Abstract

Keywords

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