Copyright (c) 2014 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Simultaneous Determination of Eight Preservatives in Feeds by High Performance Liquid Chromatography
Corresponding Author(s) : Ying-Min Jia
Asian Journal of Chemistry,
Vol. 26 No. 14 (2014): Vol 26 Issue 14
Abstract
A high performance liquid chromatography method for the separation and determination of eight preservatives benzoic acid, sorbic acid and six parabens in feeds was developed and validated. The condition of sample preparation was 50 % methanol and 50 % water (v/v) extraction of the feeds. The experiment was carried out with the mobile phase of methanol-0.05 mol/L ammonium formate aqueous solution (pH 4.5) using gradient elution at 245 nm. Under optimized conditions, the eight preservatives were separated successfully within 13 min. The correlation coefficients were more than 0.9991 and the detection limits were in the range of 0.05 and 0.5 μg/mL. The average recoveries of the eight preservatives were between 80.8 and 107.4 %. The relative standard deviations (RSDs) were less than 5.0 % (n = 5). The proposed HPLC method is sensitive, reliable, fast and can be applied to quality control test of preservatives in feeds.
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References
J.M. Tong, Feed Additive Manual, China’s Agriculture Press, Beijing, pp. 315-329 (2001).
M.R. Lee, C.Y. Lin, Z.G. Li and T.F. Tsai, J. Chromatogr. A, 1120, 244 (2006); doi:10.1016/j.chroma.2006.01.075.
T.J. Yang, F.J. Tsai, C.Y. Chen, T.C.C. Yang and M.R. Lee, J. Anal. Chim. Acta, 668, 188 (2010); doi:10.1016/j.aca.2010.04.001.
S.A.V. Tfouni and M.C.F. Toledo, J. Food Control, 13, 117 (2002); doi:10.1016/S0956-7135(01)00084-6.
G. Grosa, E.D. Grosso, R. Russo and G. Allegrone, J. Pharm. Biomed. Anal., 41, 798 (2006); doi:10.1016/j.jpba.2006.01.026.
P.Y. Wang, Y. Zhou, L. Huang, H. Zhao, A. Chen and H.Y. Zhang, J. Zhejiang Ocean Univ. (Nat. Sci.), 4, 425 (2008).
I. Martins, F.C. Carreira, L.S. Canaes, F.A. de Souza Campos Jr., L.M. da Silva Cruz and S. Rath, Talanta, 85, 1 (2011); doi:10.1016/j.talanta.2011.04.047.
W.H. Gao and C. Legido-quigley, J. Chromatogr. A, 1218, 4307 (2011); doi:10.1016/j.chroma.2011.04.064.
Y. Pan, B. Li, H.K. Zhu, X. Feng, N. Zhu and Z.R. Niu, J. Food Res. Develop., 2, 105 (2011).
J. Xia, J.X. Zhang, H. Li, L. Yang and C.M. Wu, J. Food Sci., 14, 219 (2011).
Y. Ling, X.G. Chu, F. Zhang, Q. Chen, Y. F. Zhao and Y.N. Wu, Chin. J. Chromatogr., 8, 723 (2011) (in Chinese).
M.A. Farajzadeh, Dj. Djozan and R.F. Bakhtiyari, Talanta, 81, 1360 (2010); doi:10.1016/j.talanta.2010.02.035.
H.-B. Lee, T.E. Peart and M.L. Svoboda, J. Chromatogr. A, 1094, 122 (2005); doi:10.1016/j.chroma.2005.07.070.
Q. Chen, J. R. Huang and Y. Ling, Chin. J. Anal. Chem., 5, 723 (2011) (in Chinese).
H.-Y. Huang, Y.-C. Lai, C.-W. Chiu and J.-M. Yeh, J. Chromatogr. A, 993, 153 (2003); doi:10.1016/S0021-9673(03)00395-9.
S.L. He, Y.F. Zhao, Z.W. Zhu, H.W. Liu, M.X. Li, Y.H. Shao and Q.K. Zhuang, Talanta, 69, 166 (2006); doi:10.1016/j.talanta.2005.09.018.
Y. Gao, Y. Z. He, H.Y. Xie and D.S. Ling, Chin. J. Anal. Chem., 11, 1625 (2010) (in Chinese).
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