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Simultaneous Determination of Five Active Ingredients in Yixintong Tablet by HPLC
Corresponding Author(s) : Wenting Zhang
Asian Journal of Chemistry,
Vol. 26 No. 15 (2014): Vol 26 Issue 15
Abstract
To establish an HPLC method for the simultaneous determination of five active ingredients (glucosyl-vitexin, rhamnosyl-vitexin, vitexin, rutin and hyperin). HPLC conditions included Welch material XB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of tetrahydrofuran (A)-acetonitrile (B)-methanol (C)-0.05 % phosphoric acid (D) at a flow rate of 1 mL/min for gradient elution. The column temperature was 30 °C. Five active ingredients had good linear relationships: glucosyl-vitexin in the range of 0.0369-1.85 μg (R2 = 0.9998), rhamnosyl-vitexin in the range of 0.109-5.47 μg (R2 = 0.9995), vitexin in the range of 0.00600-0.300 μg (R2 = 0.9999), rutin in the range of 0.00510-0.255 μg (R2 = 0.9997) and hyperin in the range of 0.00510-0.255 μg (R2 = 0.9997). Their average recoveries were 97.55 % (RSD = 1.9 %), 98.27 % (RSD = 2.1 %), 98.22 % (RSD = 2.4 %), 97.55 % (RSD = 2.1 %) and 98.13 % (RSD = 2.6 %), respectively. This method was steady with high precision and good repeatability. It could be used for the determination of the five active ingredients and quality control of Yixintong tablet.
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References
State Pharmacopeia Committee of China, Chinese Pharmacopoeia, 2010 version, Part I, p. 1024 (2010).
D.F. Hao, R.P. Yang, Y.Z. Zhou, H. Chen, Z.-F. Li and Y.-H. Pei, J. Shenyang Pharm. Univ., 26, 282 (2009).
R.H. Liu, F. Shao, Y.Q. Deng, et al., J. Chin. Med. Mater., 31, 1102 (2007).
Y.C. Quan and L.P. Guan, Lishizhen Med. Mater. Med. Res., 22, 463 (2006).
Q.H. Yu, P.L. Huang, S.H. Zhang and H. Wang, Chin. J. Publ. Health, 22, 463 (2006).
B. Yu, H.Y. Li, L. Zhang, L. Xu and T.H. Fang, Tradit. Chinese Drug Res. Clin. Pharmacol., 19, 461 (2008).
L. Li, H. Lv and H. Pang, Lishizhen Med. Mater. Med. Res., 18, 2143 (2007).
Q. Min, Y.T. Bai, Z. Zhang and L.-Y. Shi, Chinese J. Clin. Pharmacol. Therap., 12, 800 (2007).
Y.-S. Ji, X.-D. Zhang, H. Li and S.-J. Yang, J. Jilin Univ. (Medicine Ed.), 31, 879 (2005).
X.T. Lv, J.R. Ma and E.L. Yi, World Health Digest, 4, 526 (2007).
X.Y. Ye, L. Zhang, J. Shen and Y.F. Wang, Chin. Tradit. Herbal Drugs, 36, 1683 (2005).
P. Li, J.N. Wang, S.J. Lu, J.H. Fu and J.X. Liu, China J. Chinese Mater. Med., 34, 96 (2009).
X.Y. Ju, T.H. Fang and W.T. Zhang, J. Nanjing Univ. TCM, 21, 381 (2005).
Z.Y. Chen, M.X. Yan and P.H. He, J. Med. Res., 36, 33 (2007).
K.Y. Jang, Z.L. Gu and C.G. Ruan, Chinese Tradit. Patent Med., 22, 782 (2000).