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Vol. 26 No. 15 (2014): Vol 26 Issue 15

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Simultaneous Determination of Five Active Ingredients in Yixintong Tablet by HPLC

https://doi.org/10.14233/ajchem.2014.16221
Weiming Cheng
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou Zhejiang Province, P.R. China
Jie Gao
Zhejiang Medical University, No. 548 Binwen Road, Binjiang Dist. 310053, Hangzhou Zhejiang Province, P.R. China
Chao Yue
Zhejiang Medical University, No. 548 Binwen Road, Binjiang Dist. 310053, Hangzhou Zhejiang Province, P.R. China
Wenting Zhang
Zhejiang Institute for Food and Drug Control, No. 86 Lane 1, Jichang Road, Jianggan Dist. 310004, Hangzhou Zhejiang Province, P.R. China

Corresponding Author(s) : Wenting Zhang

leozhwt@163.com

Asian Journal of Chemistry, Vol. 26 No. 15 (2014): Vol 26 Issue 15

Abstract

To establish an HPLC method for the simultaneous determination of five active ingredients (glucosyl-vitexin, rhamnosyl-vitexin, vitexin, rutin and hyperin). HPLC conditions included Welch material XB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of tetrahydrofuran (A)-acetonitrile (B)-methanol (C)-0.05 % phosphoric acid (D) at a flow rate of 1 mL/min for gradient elution. The column temperature was 30 °C. Five active ingredients had good linear relationships: glucosyl-vitexin in the range of 0.0369-1.85 μg (R2 = 0.9998), rhamnosyl-vitexin in the range of 0.109-5.47 μg (R2 = 0.9995), vitexin in the range of 0.00600-0.300 μg (R2 = 0.9999), rutin in the range of 0.00510-0.255 μg (R2 = 0.9997) and hyperin in the range of 0.00510-0.255 μg (R2 = 0.9997). Their average recoveries were 97.55 % (RSD = 1.9 %), 98.27 % (RSD = 2.1 %), 98.22 % (RSD = 2.4 %), 97.55 % (RSD = 2.1 %) and 98.13 % (RSD = 2.6 %), respectively. This method was steady with high precision and good repeatability. It could be used for the determination of the five active ingredients and quality control of Yixintong tablet.

Keywords

HPLC Yixintong tablet Glucosyl-vitexin Rhamnosylvitexin Vitexin Rutin Hyperin
Cheng, W., Gao, J., Yue, C., & Zhang, W. (2014). Simultaneous Determination of Five Active Ingredients in Yixintong Tablet by HPLC. Asian Journal of Chemistry, 26(15), 4782–4784. https://doi.org/10.14233/ajchem.2014.16221
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