Total Phenolic, Total Flavonoid and Antioxidant Activity of Euonymus fortunei by HPLC-Based Analysis

Xi-Lin Ouyang; Xian-Ming Fang; Heng-Shan Wang; Liu-Xin Wei; Ying-Ming Pan

doi:10.14233/ajchem.2014.16159

Issue

Vol. 26 No. 15 (2014): Vol 26 Issue 15

Issue Published : July 16, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Total Phenolic, Total Flavonoid and Antioxidant Activity of Euonymus fortunei by HPLC-Based Analysis

https://doi.org/10.14233/ajchem.2014.16159

Xi-Lin Ouyang

Youjiang Medical University for Nationalities, Baise 533000, P.R. China

Xian-Ming Fang

Guangxi Traditional Chinese Medical University, Nanning 530001, P.R. China

Heng-Shan Wang

State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry & Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China

Liu-Xin Wei

State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry & Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China

Ying-Ming Pan

State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry & Pharmacy of Guangxi Normal University, Guilin 541004, P.R. China

Corresponding Author(s) : Heng-Shan Wang

hengshan_wang@yahoo.com

Asian Journal of Chemistry, Vol. 26 No. 15 (2014): Vol 26 Issue 15
Article Published : July 16, 2014

Abstract

Euonymus fortunei is one of the most important folk medicinal plants in Guangxi of China and also used as a healthy tea for enhancement of immunity and disease resistance. The ethanol crude extract and its fractions of E. fortunei were investigated to determine their total phenolic and flavonoid contents, as well as their antioxidant activity, which assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diamonium salt (ABTS) radical scavenging assay. Its' compositions were analyzed by HPLC. The ethyl acetate fraction possesses the high total phenolic and total flavonoid with the contents of 707.2 mg gallic acid equivalents and 581 mg rutin equivalents g^-1 extract, respectively. The ethyl acetate fraction also exhibited the strongest activity with EC₅₀ value of 0.25 mg/mL, followed by the n-butanol fraction, while petroleum ether and water fractions were less active than crude extract. HPLC analysis of the ethanol crude extracts indicated the presence of a large number of flavonoids and its' glycosides. E. fortunei could be used as an important natural source of antioxidants and a substantial source of healthy tea, as well as a medicinal plant.

Keywords

Euonymus fortunei Flavonoid Antioxidant HPLC analysis

Ouyang, X.-L., Fang, X.-M., Wang, H.-S., Wei, L.-X., & Pan, Y.-M. (2014). Total Phenolic, Total Flavonoid and Antioxidant Activity of Euonymus fortunei by HPLC-Based Analysis. Asian Journal of Chemistry, 26(15), 4648–4650. https://doi.org/10.14233/ajchem.2014.16159

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References

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References

Jiangsu New Medical College, Shanghai Science and Technology Press, Shanghai, China (1986).

M. Liao, Y. Yang, G. Yang and Z. Mei, J. South-Central Univ. National. (Nat. Sc. Ed.), 28, 51 (2009).

F.L. Qu, Q.L. Ding and H.M. Zhang, Med. J. National Defending Forces in Southwest Chin., 12, 349 (2002).

X.L. Ouyang, L.X. Wei, X.M. Fang, H.S. Wang and Y.M. Pan, Chem. Nat. Comp., 49, 428 (2013); doi:10.1007/s10600-013-0630-0.

X.L. Ouyang, X.M. Fang, Y.M. Pan, L.X. Wei and H.S. Wang, Acta Chromatogr., 24, 301 (2012); doi:10.1556/AChrom.24.2012.2.12.

W. Kriaa, H. Fetoui, M. Makni, N. Zeghal and N.-E. Drira, Int. J. Food Prop., 15, 1220 (2012); doi:10.1080/10942912.2010.514673.

C.I.G. Tuberoso, P. Montoro, S. Piacente, G. Corona, M. Deiana, M.A. Dessì, C. Pizza and P. Cabras, J. Pharm. Biomed. Anal., 50, 440 (2009); doi:10.1016/j.jpba.2009.05.032.

G. Miliauskas, P.R. Venskutonis and T.A. van Beek, Food Chem., 85, 231 (2004); doi:10.1016/j.foodchem.2003.05.007.

Y. Pan, K. Wang, S. Huang, H. Wang, X. Mu, C. He, X. Ji, J. Zhang and F. Huang, Food Chem., 106, 1264 (2008); doi:10.1016/j.foodchem.2007.07.033.

Y. Cai, Q. Luo, M. Sun and H. Corke, Life Sci., 74, 2157 (2004); doi:10.1016/j.lfs.2003.09.047.

J. Xie, L. Shi, X. Zhu, P. Wang, Y. Zhao and W. Su, Innov. Food Sci. Emerg. Technol., 12, 146 (2011); doi:10.1016/j.ifset.2010.12.009.

Issue

Vol. 26 No. 15 (2014): Vol 26 Issue 15

Total Phenolic, Total Flavonoid and Antioxidant Activity of Euonymus fortunei by HPLC-Based Analysis

Corresponding Author(s) : Heng-Shan Wang

Abstract

Keywords

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