Determination of Synergistic Effects of Catalase and Natural Antioxidants in Scavenging &bull;OH by Capillary Electrophoresis

Zhen Du; Chenling Fan

doi:10.14233/ajchem.2014.16622

Issue

Vol. 26 No. 16 (2014): Vol 26 Issue 16

Issue Published : July 28, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Determination of Synergistic Effects of Catalase and Natural Antioxidants in Scavenging •OH by Capillary Electrophoresis

https://doi.org/10.14233/ajchem.2014.16622

Zhen Du

Department of Pharmacy, The First Affiliated Hospital of China Medical University, No. 155 Nanjingbei Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China ; School of Pharmacy, China Medical University, No. 92 Beier Road, Heping Dist. 110001, Shenyang Liaoning Province, P.R. China

Chenling Fan

Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, Shenyang 110001, P.R. China

Corresponding Author(s) : Zhen Du

zhong-shan2000@hotmail.com

Asian Journal of Chemistry, Vol. 26 No. 16 (2014): Vol 26 Issue 16
Article Published : August 4, 2014

Abstract

A capillary electrophoresis method for the determination of serum ^•OH is established. The ascorbic acid/Fe²⁺ system was used to generate the ^•OH and the benzoic acid was used to trap the ^•OH. The optimal conditions such as the SDS concentration, buffer pH and separation voltage were investigated under the optimal condition, the impacts of substrate concentrations and reaction time towards the production of ^•OH were studied. All the substances could be well separated basically; the linear range of the reaction product (m-hydroxylbenzoic acid) was 5 × 10^-6 to 3 × 10^-3 mol/L and the limit of detection was 1.0 × 10^-6 mol/L. Then the method was applied to determine the IC₅₀ of catalase, catechin and quercetin towards the serum ^•OH. The method was stable and quick and could be specifically applied into the research of the synergistic effects of catalase and natural antioxidants in scavenging the serum ^•OH.

Keywords

Capillary electrophoresis Catalase Catechin Quercetin Hydroxyl radical Synergistic antioxidant

Du, Z., & Fan, C. (2014). Determination of Synergistic Effects of Catalase and Natural Antioxidants in Scavenging •OH by Capillary Electrophoresis. Asian Journal of Chemistry, 26(16), 5171–5173. https://doi.org/10.14233/ajchem.2014.16622

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A. Natarajan, C. Chun, J.J. Hickman and P. Molnar, J. Biomater. Sci. Polym. Ed., 19, 1319 (2008); doi:10.1163/156856208786052399.

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