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Vol. 26 No. 19 (2014): Vol 26 Issue 19

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Ultrasensitive Impedimetric Lectin Biosensor with Efficient Antifouling Properties Applied in Determination of a-Fetoprotein on Mixed Self-Assembled Monolayer on Gold

https://doi.org/10.14233/ajchem.2014.16372
Haiying Yang
Department of Applied Chemistry, Yuncheng University, Yuncheng 044000, P.R. China
Qiaojuan Gong
Department of Applied Chemistry, Yuncheng University, Yuncheng 044000, P.R. China
Lina Ma
Department of Applied Chemistry, Yuncheng University, Yuncheng 044000, P.R. China
Yuanlin Sun
Department of Life Science, Yuncheng University, Yuncheng 044000, P.R. China
Wenchan Zhang
Department of Applied Chemistry, Yuncheng University, Yuncheng 044000, P.R. China

Corresponding Author(s) : Haiying Yang

hyyang@ycu.edu.cn

Asian Journal of Chemistry, Vol. 26 No. 19 (2014): Vol 26 Issue 19

Abstract

A sensitive and attractive antifouling impedimetric lectin biosensor for the determination of a-fetoprotein in human serum samples was developed. The biosensor was fabricated by co-assembling 11-mercapto-undecanoic acid for covalent immobilization of wheat-germ agglutinin and dithiothreitol to resist nonspecific interactions on the surface of gold electrode. In the measurement of a-fetoprotein, the change in interfacial electron transfer resistance of biosensor was monitored using a ferri/ferrocyanide redox couple. Owing to the specific recognition of wheat-germ agglutinin with N-glycan on a-fetoprotein, the increase in the electron transfer resistance was linearly proportional to the logarithm of the concentration of a-fetoprotein in the range from 1.0 × 10-11 to 8 × 10-10 g mL-1 and a detection limit of 9 × 10-12 g mL-1 a-fetoprotein was achieved. The fabricated biosensor allowed direct quantification of extrinsic a-fetoprotein in serum samples. This work provides a promising strategy for clinical application with impressive sensitivity and antifouling characteristics.

Keywords

Impedimetric Lectin biosensor Mixed self-assembled monolayer α-Fetoprotein
Yang, H., Gong, Q., Ma, L., Sun, Y., & Zhang, W. (2014). Ultrasensitive Impedimetric Lectin Biosensor with Efficient Antifouling Properties Applied in Determination of a-Fetoprotein on Mixed Self-Assembled Monolayer on Gold. Asian Journal of Chemistry, 26(19), 6357–6363. https://doi.org/10.14233/ajchem.2014.16372
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