Isolation, Characterisation and in silico Studies of Bioactive  Terpenoid and Flavonoid from the Seeds of Mucuna pruriens

Ranajit Kumar Sutradhar; Pair Ahmed; Md. Sakhaoyat Hossain; Md. Nazrul Islam; Md. Emdad Hossain

doi:10.14233/ajchem.2026.35289

Issue

Vol. 38 No. 4 (2026): Vol 38 Issue 4, 2026

Issue Published : 8 April 2026

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation, Characterisation and in silico Studies of Bioactive Terpenoid and Flavonoid from the Seeds of Mucuna pruriens

https://doi.org/10.14233/ajchem.2026.35289

Ranajit Kumar Sutradhar

Department of Chemistry, Chittagong University of Engineering & Technology, Chattogram-4349, Bangladesh

https://orcid.org/0000-0002-6020-0528

Pair Ahmed

Department of Chemistry, Chittagong University of Engineering & Technology, Chattogram-4349, Bangladesh

Md. Sakhaoyat Hossain

Department of Chemistry, Chittagong University of Engineering & Technology, Chattogram-4349, Bangladesh

Md. Nazrul Islam

Nuclear Power Plant Company Lid. Rooppur, Ishwardi, Bangladesh

Md. Emdad Hossain

Wazed Miah Science Research Center, Jahangirnagar University, Savar, Dhaka, Bangladesh

Corresponding Author(s) : Ranajit Kumar Sutradhar

rksutradhar2002@yahoo.com

Asian Journal of Chemistry, Vol. 38 No. 4 (2026): Vol 38 Issue 4, 2026
Article Published : 8 April 2026

Abstract

In this work, new bioactive terpenoids (1-2) and flavonoid (3) have been isolated from the chloroform extract of the seeds of Mucuna pruriens. The structures of the isolated compounds were established by spectroscopic analysis. In vitro antibacterial and antifungal activities of these compounds have been studied. Compound 2 showed the highest inhibition value (16.0 ± 0.5 mm) against S. aureus and (19.3 ± 0.6 mm) against B. subtilis. Compound 2 also exhibited significant antifungal activities with inhibition value (16.0 ± 0.6) mm and (6.0 ± 1.0) mm against T. harzianum and A. niger, respectively. Molecular docking studies and ADME prediction of the compounds were performed. Compound 2 showed good binding affinity ranging between -7.5 to -7.6 kcal/mol with targeted receptors.

Keywords

Mucuna pruriens Triterpenoid Flavonoid Bioactivity Molecular docking

(1)

Sutradhar, R. K.; Ahmed, P.; Hossain, M. S.; Islam, M. N.; Hossain, M. E. Isolation, Characterisation and in Silico Studies of Bioactive Terpenoid and Flavonoid from the Seeds of Mucuna Pruriens. ajc 2026, 38, 1087-1094.

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P.K. Sadh, P. Chawla and J.S. Duhan, Food Biosci., 22, 113 (2018); https://doi.org/10.1016/j.fbio.2018.01.011

S.O. Majekodunmi, A.A. Oyagbemi, S. Umukoro and O.A. Odeku, Asian Pac. J. Trop. Med., 4, 632 (2011); https://doi.org/10.1016/S1995-7645(11)60161-2

Y. Rajeshwar, M. Gupta and U.K. Mazumder, Iranian J. Pharmacol. Therap., 4, 46 (2005).

S.L. Johnson, H.Y. Park, N.A. DaSilva, D.A. Vattem, H. Ma and N.P. Seeram, Nutrients, 10, 1139 (2018); https://doi.org/10.3390/nu10091139

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M.A. Jimoh, O.A. Idris and M.O. Jimoh, Plants, 9, 1249 (2020); https://doi.org/10.3390/plants9091249

T. Dhanani, R. Singh, S. Shah, P. Kumari and S. Kumar, Green Chem. Lett. Rev., 8, 43 (2015); https://doi.org/10.1080/17518253.2015.1075070

G. Shanmugavel and G. Krishnamoorthy, Int. J. Herb. Med., 2, 7 (2015).

A.K. Rayavarapu and D.S. Kaladhar, Asian J. Biochem. Pharm. Res., 12, 593 (2011).

N.H. Tan, S.Y. Fung, S.M. Sim, E. Marinello, R. Guerranti and J.C. Aguiyi, J. Ethnopharmacol., 123, 356 (2009); https://doi.org/10.1016/j.jep.2009.03.025

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Y. Lin, W.-J. Tsai, I.-S. Chen and Y.-H. Kuo, J. Chin. Chem. Soc., 45, 213 (1998); https://doi.org/10.1002/jccs.199800035

A.J. Demuner, L.C.A. Barbosa, J.C. Nascimento, J.J. Vieira and M.A. Santos, Quim. Nova, 26, 335 (2003); https://doi.org/10.1590/S0100-40422003000300009

L.T. Renata, S.S. Alexandre, R.N.C. Ana, R.P.S. Alexandre and S.A. Jailane, Afr. J. Biotechnol., 14, 676 (2015); https://doi.org/10.5897/AJB2014.14354

D. Das, S. Das, M. Pandey and D. Bhattacharyay, Eur. J. Med. Plants, 31, 19 (2020); https://doi.org/10.9734/ejmp/2020/v31i430226

L. Misra and H. Wagner, Phytochemistry, 65, 2565 (2004); https://doi.org/10.1016/j.phytochem.2004.08.045

J.B. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Dordrecht, Netherlands: Springer, edn 3 (1998).

M. Singh, I.P. Kumhar and M. Salim, J. Med. Plants Stud., 10, 103 (2022).

A.W. Bauer, W.M. Kirby, J.C. Sherris and M. Turck, Am. J. Clin. Pathol., 45(4_ts), 493 (1966); https://doi.org/10.1093/ajcp/45.4_ts.493

D.S. Goodsell, M.F. Sanner, A.J. Olson and S. Forli, Protein Sci., 30, 31 (2021); https://doi.org/10.1002/pro.3934

H.M. Berman, Nucleic Acids Res., 28, 235 (2000); https://doi.org/10.1093/nar/28.1.235

O. Trott and A.J. Olson, J. Comput. Chem., 31, 455 (2010); https://doi.org/10.1002/jcc.21334

M.M. Alnoman, S. Parveen, R.B. Alnoman, A. Khan, M.M. Khaleil, M. Jaremko, I. Al-Younis and A.-H. Emwas, J. Mol. Struct., 1307, 138021 (2024); https://doi.org/10.1016/j.molstruc.2024.138021

Author biographies is not available.