Copyright (c) 2026 Namita Joshi

This work is licensed under a Creative Commons Attribution 4.0 International License.
Phytochemical Screening, Isolation and Characterisation of Phytoconstituents of Lagerstroemia lanceolata
Corresponding Author(s) : Namita Joshi
Asian Journal of Chemistry,
Vol. 38 No. 4 (2026): Vol 38 Issue 4, 2026
Abstract
The present study focuses on the phytochemical screening, isolation and characterisation of phytoconstituents from Lagerstroemia lanceolata, a medicinally significant species known for its diverse therapeutic applications. Preliminary phytochemical evaluation of the methanolic extract confirmed the presence of major secondary metabolites, prompting further purification. Thin layer chromatography (TLC) was employed to optimize the solvent system for separation, where the mobile phase toluene:ethyl acetate (7.5:2.5) provided superior resolution of phytoconstituents. Based on the TLC analysis, column chromatography resulted in the isolation of three pentacyclic triterpenoids, namely oleanolic acid, ursolic acid and corosolic acid. Structural elucidation and confirmation of purity were achieved using a combination of spectroscopic techniques, including IR, 1H NMR, 13C NMR and LC-MS.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- Z. Yue, Y. Xu, M. Cai, X. Fan, H. Pan, D. Zhang and Q. Zhang, Plants, 13, 3016 (2024); https://doi.org/10.3390/plants13213016 DOI: https://doi.org/10.3390/plants13213016
- H. Jaisinghani and P. Magarde, J. Med. Plants Stud., 10, 27 (2022).
- A. Gupta, V. K. Agrawal and C.V. Rao, J. Appl. Pharm. Sci., 7, 156 (2017); https://doi.org/10.7324/JAPS.2017.70221
- G. Klein, J. Kim, K. Himmeldirk, Y. Cao and X. Chen, Evid.-Based Complement. Altern. Med., 4, 401 (2007); https://doi.org/10.1093/ecam/nem013 DOI: https://doi.org/10.1093/ecam/nem013
- M.S. Haque, R. Ahmad, B.C. Sarkar, M. Sana, H.R. Saha and N.K. Sana, PLoS One, 20, e0339566 (2025); https://doi.org/10.1371/journal.pone.0339566 DOI: https://doi.org/10.1371/journal.pone.0339566
- T.T. Priya, M.C. Sabu and C.I. Jolly, Inflammopharmacology, 16, 182 (2008); https://doi.org/10.1007/s10787-008-7002-6 DOI: https://doi.org/10.1007/s10787-008-7002-6
- E.J. Yang, J.S. Lee, B.B. Song, C.Y. Yun, D.H. Kim and I.S. Kim, J. Ethnopharmacol., 136, 422 (2011); https://doi.org/10.1016/j.jep.2010.05.066 DOI: https://doi.org/10.1016/j.jep.2010.05.066
- S.W. Jadhav, B. Ramachandra and S.R. Avanapu, Der Pharm. Sin., 6, 67 (2015).
- M.S. Sikarwar, L.C. Chung, L.W. Ting, L.C. Chee, S. Fuloria and K. Balaji, J. Appl. Pharm. Sci., 6, 185 (2016); https://doi.org/10.7324/JAPS.2016.60830 DOI: https://doi.org/10.7324/JAPS.2016.60830
- T. Van Loc, N.T. Ngan, P.T. Ninh, T.T. Thao, N.T. Luu, N.M. Thu, N.T. Van Anh and T. Van Sung, Vietnam J. Chem., 53, 243 (2015); https://doi.org/10.15625/0866-7144.2015-00123
- P. Budholiya and H.K. Sharma, J. Drug Deliv. Ther., 9, 674 (2019); https://doi.org/10.22270/jddt.v9i4-A.3550 DOI: https://doi.org/10.22270/jddt.v9i4-A.3550
- G.H. Huang, Q. Zhan, J.L. Li, C. Chen, D.D. Huang, W.S. Chen and L.N. Sun, Biochem. Syst. Ecol., 51, 109 (2013); https://doi.org/10.1016/j.bse.2013.08.029 DOI: https://doi.org/10.1016/j.bse.2013.08.029
- O.G. Bhusnure, K.R. Alagawadi, P.S. Giram and B.N. Poul, Int. J. Pharm. Chem. Res., 1, 127 (2009).
- M. Adil, A.D. Kandhare, G. Dalvi, P. Ghosh, S. Venkata and K.S. Raygude, Ren. Fail., 38, 996 (2016); https://doi.org/10.3109/0886022X.2016.1165120 DOI: https://doi.org/10.3109/0886022X.2016.1165120
- M. Adil, A.D. Kandhare, A. Visnagri and S.L. Bodhankar, Ren. Fail., 37, 1396 (2015); https://doi.org/10.3109/0886022X.2015.1074462 DOI: https://doi.org/10.3109/0886022X.2015.1074462
- S.L. Badole, S.M. Chaudhari, G.B. Jangam, A.D. Kandhare and S.L. Bodhankar, Biomed. Res. Int., 2015, 403291 (2015); https://doi.org/10.1155/2015/403291 DOI: https://doi.org/10.1155/2015/403291
- S.T. Devkar, A.D. Kandhare, A.A. Zanwar, S.D. Jagtap, S.S. Katyare and S.L. Bodhankar, Pharm. Biol., 54, 2394 (2016); https://doi.org/10.3109/13880209.2016.1157193 DOI: https://doi.org/10.3109/13880209.2016.1157193
- A.D. Kandhare, J. Alam, M.V. Patil, A. Sinha and S.L. Bodhankar, Pharm. Biol., 54, 419 (2016); https://doi.org/10.3109/13880209.2015.1038755 DOI: https://doi.org/10.3109/13880209.2015.1038755
- A.D. Kandhare, U.M. Aswar, V. Mohan and P.A. Thakurdesai, Anat. Cell Biol., 50, 275 (2017); https://doi.org/10.5115/acb.2017.50.4.275 DOI: https://doi.org/10.5115/acb.2017.50.4.275
- A.D. Kandhare, S.L. Bodhankar, V. Mohan and P.A. Thakurdesai, Chem.-Biol. Interact., 237, 151 (2015); https://doi.org/10.1016/j.cbi.2015.06.019 DOI: https://doi.org/10.1016/j.cbi.2015.06.019
- A.D. Kandhare, P. Ghosh and S.L. Bodhankar, Chem.-Biol. Interact., 219, 101 (2014); https://doi.org/10.1016/j.cbi.2014.05.012 DOI: https://doi.org/10.1016/j.cbi.2014.05.012
- A. Al Groshi, L. Nahar, F.M. Ismail, A.R. Evans and S.D. Sarker, Dhaka Univ. J. Pharm. Sci., 20, 267 (2022); https://doi.org/10.3329/dujps.v20i3.59792 DOI: https://doi.org/10.3329/dujps.v20i3.59792
- P. Srivastava and R. Chaturvedi, In Vitro Cell. Dev. Biol.-Plant, 46, 549 (2010); https://doi.org/10.1007/s11627-010-9298-3 DOI: https://doi.org/10.1007/s11627-010-9298-3
- D.D. Tshilanda, D.N. Onyamboko, P. Babady-Bila, K.T. Ngbolua, D.S. Tshibangu, E.F. Dibwe and P.T. Mpiana, Nat. Prod. Bioprospect., 5, 215 (2015); https://doi.org/10.1007/s13659-015-0070-6 DOI: https://doi.org/10.1007/s13659-015-0070-6
- J.A. Sawale, J.R. Patel and M.L. Kori, J. Pharmacogn. Phytochem., 6, 936 (2017).
- J.M. Castellano, S. Ramos-Romero and J.S. Perona, Nutrients, 14, 623 (2022); https://doi.org/10.3390/nu14030623 DOI: https://doi.org/10.3390/nu14030623
- D. Palu, A. Bighelli, J. Casanova and M. Paoli, Molecules, 24, 4413 (2019); https://doi.org/10.3390/molecules24234413 DOI: https://doi.org/10.3390/molecules24234413
- J. Pollier and A. Goossens, Phytochemistry, 77, 10 (2012); https://doi.org/10.1016/j.phytochem.2011.12.022 DOI: https://doi.org/10.1016/j.phytochem.2011.12.022
- H.R. Naika, S. Bhavana, J.A. Teixeira da Silva, K. Lingaraju, V.C. Mohan and V. Krishna, Nusantara Biosci., 8, 232 (2016); https://doi.org/10.1305s7/nusbiosci/n080216 DOI: https://doi.org/10.13057/nusbiosci/n080216
- S. Xu, G. Wang, W. Peng, Y. Xu, Y. Zhang, Y. Ge, Y. Jing and Z. Gong, Sci. Rep., 9, 4388 (2019); https://doi.org/10.1038/s41598-019-40934-7 DOI: https://doi.org/10.1038/s41598-019-40934-7
- N. Bharadwaj and A. Chaturvedi, J. Adv. Sci. Res., 12, 280 (2021); https://doi.org/10.55218/JASR.202112438 DOI: https://doi.org/10.55218/JASR.202112438
- S.W. Jadhav R.B. Jadhav and A. Srinivas Rao, Scholars Acad. J. Pharm., 7, 474 (2018).
- N.A. Luchnikova, V.V. Grishko and I. B. Ivshina, Molecules, 25, 5526 (2020); https://doi.org/10.3390/molecules25235526 DOI: https://doi.org/10.3390/molecules25235526
- F. Yan, Y. Huang, Y. Wang, Q. Li and X. He, Ind. Crops Prod., 130, 363 (2019); https://doi.org/10.1016/j.indcrop.2019.01.004 DOI: https://doi.org/10.1016/j.indcrop.2019.01.004
- Z. Jiang, W. Zhang, Y. Wang, J. Xu and X. He, Phytochemistry, 236, 114511 (2025); https://doi.org/10.1016/j.phytochem.2025.114511 DOI: https://doi.org/10.1016/j.phytochem.2025.114511
References
Z. Yue, Y. Xu, M. Cai, X. Fan, H. Pan, D. Zhang and Q. Zhang, Plants, 13, 3016 (2024); https://doi.org/10.3390/plants13213016 DOI: https://doi.org/10.3390/plants13213016
H. Jaisinghani and P. Magarde, J. Med. Plants Stud., 10, 27 (2022).
A. Gupta, V. K. Agrawal and C.V. Rao, J. Appl. Pharm. Sci., 7, 156 (2017); https://doi.org/10.7324/JAPS.2017.70221
G. Klein, J. Kim, K. Himmeldirk, Y. Cao and X. Chen, Evid.-Based Complement. Altern. Med., 4, 401 (2007); https://doi.org/10.1093/ecam/nem013 DOI: https://doi.org/10.1093/ecam/nem013
M.S. Haque, R. Ahmad, B.C. Sarkar, M. Sana, H.R. Saha and N.K. Sana, PLoS One, 20, e0339566 (2025); https://doi.org/10.1371/journal.pone.0339566 DOI: https://doi.org/10.1371/journal.pone.0339566
T.T. Priya, M.C. Sabu and C.I. Jolly, Inflammopharmacology, 16, 182 (2008); https://doi.org/10.1007/s10787-008-7002-6 DOI: https://doi.org/10.1007/s10787-008-7002-6
E.J. Yang, J.S. Lee, B.B. Song, C.Y. Yun, D.H. Kim and I.S. Kim, J. Ethnopharmacol., 136, 422 (2011); https://doi.org/10.1016/j.jep.2010.05.066 DOI: https://doi.org/10.1016/j.jep.2010.05.066
S.W. Jadhav, B. Ramachandra and S.R. Avanapu, Der Pharm. Sin., 6, 67 (2015).
M.S. Sikarwar, L.C. Chung, L.W. Ting, L.C. Chee, S. Fuloria and K. Balaji, J. Appl. Pharm. Sci., 6, 185 (2016); https://doi.org/10.7324/JAPS.2016.60830 DOI: https://doi.org/10.7324/JAPS.2016.60830
T. Van Loc, N.T. Ngan, P.T. Ninh, T.T. Thao, N.T. Luu, N.M. Thu, N.T. Van Anh and T. Van Sung, Vietnam J. Chem., 53, 243 (2015); https://doi.org/10.15625/0866-7144.2015-00123
P. Budholiya and H.K. Sharma, J. Drug Deliv. Ther., 9, 674 (2019); https://doi.org/10.22270/jddt.v9i4-A.3550 DOI: https://doi.org/10.22270/jddt.v9i4-A.3550
G.H. Huang, Q. Zhan, J.L. Li, C. Chen, D.D. Huang, W.S. Chen and L.N. Sun, Biochem. Syst. Ecol., 51, 109 (2013); https://doi.org/10.1016/j.bse.2013.08.029 DOI: https://doi.org/10.1016/j.bse.2013.08.029
O.G. Bhusnure, K.R. Alagawadi, P.S. Giram and B.N. Poul, Int. J. Pharm. Chem. Res., 1, 127 (2009).
M. Adil, A.D. Kandhare, G. Dalvi, P. Ghosh, S. Venkata and K.S. Raygude, Ren. Fail., 38, 996 (2016); https://doi.org/10.3109/0886022X.2016.1165120 DOI: https://doi.org/10.3109/0886022X.2016.1165120
M. Adil, A.D. Kandhare, A. Visnagri and S.L. Bodhankar, Ren. Fail., 37, 1396 (2015); https://doi.org/10.3109/0886022X.2015.1074462 DOI: https://doi.org/10.3109/0886022X.2015.1074462
S.L. Badole, S.M. Chaudhari, G.B. Jangam, A.D. Kandhare and S.L. Bodhankar, Biomed. Res. Int., 2015, 403291 (2015); https://doi.org/10.1155/2015/403291 DOI: https://doi.org/10.1155/2015/403291
S.T. Devkar, A.D. Kandhare, A.A. Zanwar, S.D. Jagtap, S.S. Katyare and S.L. Bodhankar, Pharm. Biol., 54, 2394 (2016); https://doi.org/10.3109/13880209.2016.1157193 DOI: https://doi.org/10.3109/13880209.2016.1157193
A.D. Kandhare, J. Alam, M.V. Patil, A. Sinha and S.L. Bodhankar, Pharm. Biol., 54, 419 (2016); https://doi.org/10.3109/13880209.2015.1038755 DOI: https://doi.org/10.3109/13880209.2015.1038755
A.D. Kandhare, U.M. Aswar, V. Mohan and P.A. Thakurdesai, Anat. Cell Biol., 50, 275 (2017); https://doi.org/10.5115/acb.2017.50.4.275 DOI: https://doi.org/10.5115/acb.2017.50.4.275
A.D. Kandhare, S.L. Bodhankar, V. Mohan and P.A. Thakurdesai, Chem.-Biol. Interact., 237, 151 (2015); https://doi.org/10.1016/j.cbi.2015.06.019 DOI: https://doi.org/10.1016/j.cbi.2015.06.019
A.D. Kandhare, P. Ghosh and S.L. Bodhankar, Chem.-Biol. Interact., 219, 101 (2014); https://doi.org/10.1016/j.cbi.2014.05.012 DOI: https://doi.org/10.1016/j.cbi.2014.05.012
A. Al Groshi, L. Nahar, F.M. Ismail, A.R. Evans and S.D. Sarker, Dhaka Univ. J. Pharm. Sci., 20, 267 (2022); https://doi.org/10.3329/dujps.v20i3.59792 DOI: https://doi.org/10.3329/dujps.v20i3.59792
P. Srivastava and R. Chaturvedi, In Vitro Cell. Dev. Biol.-Plant, 46, 549 (2010); https://doi.org/10.1007/s11627-010-9298-3 DOI: https://doi.org/10.1007/s11627-010-9298-3
D.D. Tshilanda, D.N. Onyamboko, P. Babady-Bila, K.T. Ngbolua, D.S. Tshibangu, E.F. Dibwe and P.T. Mpiana, Nat. Prod. Bioprospect., 5, 215 (2015); https://doi.org/10.1007/s13659-015-0070-6 DOI: https://doi.org/10.1007/s13659-015-0070-6
J.A. Sawale, J.R. Patel and M.L. Kori, J. Pharmacogn. Phytochem., 6, 936 (2017).
J.M. Castellano, S. Ramos-Romero and J.S. Perona, Nutrients, 14, 623 (2022); https://doi.org/10.3390/nu14030623 DOI: https://doi.org/10.3390/nu14030623
D. Palu, A. Bighelli, J. Casanova and M. Paoli, Molecules, 24, 4413 (2019); https://doi.org/10.3390/molecules24234413 DOI: https://doi.org/10.3390/molecules24234413
J. Pollier and A. Goossens, Phytochemistry, 77, 10 (2012); https://doi.org/10.1016/j.phytochem.2011.12.022 DOI: https://doi.org/10.1016/j.phytochem.2011.12.022
H.R. Naika, S. Bhavana, J.A. Teixeira da Silva, K. Lingaraju, V.C. Mohan and V. Krishna, Nusantara Biosci., 8, 232 (2016); https://doi.org/10.1305s7/nusbiosci/n080216 DOI: https://doi.org/10.13057/nusbiosci/n080216
S. Xu, G. Wang, W. Peng, Y. Xu, Y. Zhang, Y. Ge, Y. Jing and Z. Gong, Sci. Rep., 9, 4388 (2019); https://doi.org/10.1038/s41598-019-40934-7 DOI: https://doi.org/10.1038/s41598-019-40934-7
N. Bharadwaj and A. Chaturvedi, J. Adv. Sci. Res., 12, 280 (2021); https://doi.org/10.55218/JASR.202112438 DOI: https://doi.org/10.55218/JASR.202112438
S.W. Jadhav R.B. Jadhav and A. Srinivas Rao, Scholars Acad. J. Pharm., 7, 474 (2018).
N.A. Luchnikova, V.V. Grishko and I. B. Ivshina, Molecules, 25, 5526 (2020); https://doi.org/10.3390/molecules25235526 DOI: https://doi.org/10.3390/molecules25235526
F. Yan, Y. Huang, Y. Wang, Q. Li and X. He, Ind. Crops Prod., 130, 363 (2019); https://doi.org/10.1016/j.indcrop.2019.01.004 DOI: https://doi.org/10.1016/j.indcrop.2019.01.004
Z. Jiang, W. Zhang, Y. Wang, J. Xu and X. He, Phytochemistry, 236, 114511 (2025); https://doi.org/10.1016/j.phytochem.2025.114511 DOI: https://doi.org/10.1016/j.phytochem.2025.114511