Issue

Vol. 38 No. 3 (2026): Vol 38 Issue 3, 2026

Copyright (c) 2026 sunil gawali, P.B. Abhange, Ram S. Barkule, A.V. Raut, D.V. Mane

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Synthesis of Pure and Ag-doped CuFe2O4 Nanoparticles using a Microwave-Assisted Biosynthesis Method and Investigation of their Antioxidant and Antimicrobial Activity

https://doi.org/10.14233/ajchem.2026.35026
Sunil P. Gawali
Department of Chemistry, Sundarrao More Arts, Commerce and Science College, Poladpur, Raigad-402303, India; School of Science, Yashwantrao Chavan Maharashtra Open University, Nashik-402222, India
https://orcid.org/0009-0001-6465-5247
P.B. Abhange
Department of Physics, G.M. Vedak College of Science, Tala Raigad-402111, India
https://orcid.org/0009-0008-8598-6185
Ram S. Barkule
Department of Physics, Sundarrao More Arts, Commerce and Science College, Poladpur, Raigad-402303, India
https://orcid.org/0000-0001-5438-583X
A.V. Raut
Department of Physics, Vivekanand Arts, Sardar Dalip Singh Commerce and Science College, Aurangabad-431001, India
https://orcid.org/0000-0002-0275-9729
D.V. Mane
School of Science, Yashwantrao Chavan Maharashtra Open University, Nashik-402222, India; Department of Chemistry, Shri Chhatrapati Shivaji College Omerga, Dharashiv-413606, India
https://orcid.org/0000-0003-3078-8284

Corresponding Author(s) : Sunil P. Gawali

spgawali1986@gmail.com

Asian Journal of Chemistry, Vol. 38 No. 3 (2026): Vol 38 Issue 3, 2026

Abstract

This study reports the successful synthesis and characterisation of silver-doped copper ferrites nanoparticles (Agx​Cu1-xFe2​O4, where x = 0.05, 0.1, 0.2) through microwave-assisted biosynthesis employing Asteracantha longifolia leaf extract as a reducing and stabilizing agent. X-ray diffraction analysis (XRD) confirmed the formation of single-phase cubic spinel structures with systematic lattice parameter variations from 8.294 to 8.165 Å upon silver incorporation. Electron microscopy (SEM) revealed morphological evolution from spherical to irregular geometries with crystallite sizes ranging between 15.43-18.85 nm. The substitutional mechanism of Ag+ ions into tetrahedral and octahedral sites was evidenced by structural refinement, demonstrating successful dopant integration without phase segregation. Spectroscopic investigations revealed enhanced optical absorption in the UV-visible region (300-800 nm) due to surface plasmon resonance effects of silver nanodomains. Magnetic characterisation showed decreased saturation magnetisation values correlating with silver content, attributed to the diamagnetic nature of Ag+ ions disrupting magnetic exchange interactions. Thermal gravimetric analysis indicated structural stability up to 600 ºC with minimal mass loss. Antioxidant evaluation through DPPH radical scavenging demonstrated concentration-dependent activity enhancement, with Ag0.2Cu0.8Fe2O4 exhibiting 55.35% radical neutralisation efficiency compared to 41.87% for undoped copper ferrite. The improved performance stems from increased electron-donating capacity facilitated by the electronic properties of silver, establishing these nanocomposites as promising materials for advanced oxidative stress mitigation applications in biomedical and environmental sectors.

Keywords

Asteracantha longifolia Microwave assisted sol-gel synthesis Ferrites Antimicrobial activity
(1)
Gawali, S. P.; Abhange, P.; Barkule, R. S.; Raut, A.; Mane, D. Synthesis of Pure and Ag-Doped CuFe2O4 Nanoparticles Using a Microwave-Assisted Biosynthesis Method and Investigation of Their Antioxidant and Antimicrobial Activity. ajc 2026, 38, 589-600.
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. T. Dippong, E.A. Levei and O. Cadar, Nanomaterials, 11, 1560 (2021); https://doi.org/10.3390/nano11061560
  2. M.M. Rashad, R.M. Mohamed, M. A. Ibrahim, L.F.M. Ismail and E.A. Abdel-Aal, Adv. Powder Technol., 23, 315 (2012); https://doi.org/10.1016/j.apt.2011.04.005
  3. A. Soufi, H. Hajjaoui, R. Elmoubarki, M. Abdennouri, S. Qourzal and N. Barka, Appl. Surf. Sci. Adv., 6, 100145 (2021); https://doi.org/10.1016/j.apsadv.2021.100145
  4. A.E. Danks, S.R. Hall and Z. Schnepp, Mater. Horiz., 3, 91 (2016); https://doi.org/10.1039/C5MH00260E
  5. B. Niu, F. Zhang, H. Ping, N. Li, J. Zhou, L. Lei, J. Xie, J. Zhang, W. Wang and Z. Fu, Sci. Rep., 7, 3421 (2017); https://doi.org/10.1038/s41598-017-03644-6
  6. K.B. Joshi, M.B. Nariya and B.J. Patgiri, Int. J. Pharm. Pharmceut. Sci., 14, 60 (2022); https://doi.org/10.22159/ijpps.2022v14i11.46148
  7. A.K. Dash, G.K. Dutta, G. Sahoo, S.K. Mishra, K.K. Sardar and P.C. Bisoi, J. Med. Plants Res., 6, 3786 (2012).
  8. S.R. Saple, S.E. Raval and V.V. Vaidya, Asian J. Pharm. Res. Health Care, 13, 197 (2021); https://doi.org/10.18311/ajprhc/2021/27448
  9. N. Phuyal, Ph.D. Thesis, The Chemical Characteristics and Antibacterial Activity of Green Tea (Camellia sinensis), Stinging Nettle (Urtica diocia) Leaves and the Blend, Department of Microbiology, Central Campus of Technology, Tribhuvan University, Dharan, Nepal (2021).
  10. T. Misra, R. Singh, H. Pandey, B. Singh and R. Pandey, Fitoterapia, 72, 194 (2001); https://doi.org/10.1016/S0367-326X(00)00269-0
  11. D. Veerakumar and M. Muthulingam, J. Pharm. Res. Int., 33, 80 (2021); https://doi.org/10.9734/jpri/2021/v33i19B31340
  12. O.Q. Adiamo, A.D.T. Phan, S. Akter, D. Sivakumar, P. Cunningham, F. Seke and Y. Sultanbawa, Int. J. Food Sci. Technol., 59, 573 (2024); https://doi.org/10.1111/ijfs.16572
  13. S. Rao, J. Cell Tissue Res., 13, 3877 (2013).
  14. A.K. Shukla, R.K. Malviya and J.N. Mishra, Int. J. Health Sci., 6S1, 14361 (2022); https://doi.org/10.53730/ijhs.v6nS1.8682
  15. N.S. Chauhan and V.K. Dixit, Rev. Bras. Farmacogn., 20, 812 (2010); https://doi.org/10.1590/S0102-695X2010005000022
  16. Z. Sun, L. Liu, D. Jia and W. Pan, Sens. Actuators B Chem., 125, 144 (2007); https://doi.org/10.1016/j.snb.2007.01.050
  17. S. Anandan, T. Selvamani, G.G. Prasad, A. M. Asiri and J. J. Wu, J. Magn. Magn. Mater., 432, 437 (2017); https://doi.org/10.1016/j.jmmm.2017.02.026
  18. D.V. Nair, N.B. Shridhar and K. Jayakumar, Int. J. Nutr. Pharmacol. Neurol. Dis., 5, 28 (2015); https://doi.org/10.4103/2231-0738.150072
  19. N. Gammatantrawet, C.T. Nguyễn, C. Susawaengsup, A.N.M. Ramli, K. Tongkoom, T. Chatsungnoen, R. Dangtungee and P. Bhuyar, Mol. Biotechnol., 67, 885 (2025); https://doi.org/10.1007/s12033-024-01122-9
  20. N. Chaudhary, Z. Roy, R. Bansal and L. Siddiqui, Curr. Pharm. Biotechnol., 24, 1265 (2023); https://doi.org/10.2174/1389201024666221121160822
  21. K.N. Sunil Kumar, K.G. Divya, R. Mattummal, P. Sathiyarajeswaran, B. Erni and K. Kanakavalli, Indian J. Pharm. Educ. Res., 55, 36 (2021); https://doi.org/10.5530/ijper.55.1.7
  22. S. Dubey, T. Virmani, S.K. Yadav, A. Sharma, G. Kumar and A. Alhalmi, J. Nanomater., 2024, 9914079 (2024); https://doi.org/10.1155/2024/9914079
  23. A.M. Mohamed, Academia Eng., 2024, 1 (2024); https://doi.org/10.20935/AcadEng7302
  24. S. Nayak, P. Ghugare and B. Vaidhun, Indian J. Pharm. Educ. Res, 55(2s), s510 (2021); https://doi.org/10.5530/ijper.55.2s.122
  25. B. Uma, K.S. Anantharaju, B. Surendra, K. Gurushantha, S.S. More, S. Meena, B. Hemavathi and H.A. Murthy, ACS Omega, 8, 9947 (2023); https://doi.org/10.1021/acsomega.2c07124
  26. H. Jiao, G. Jiao and J. Wang, Synth. React. Inorg. Met.-Org. Nano-Met. Chem., 43, 131 (2013); https://doi.org/10.1080/15533174.2012.680090
  27. N. El Messaoudi, Z. Ciğeroğlu, Z.M. Şenol, E.S. Kazan-Kaya, Y. Fernine, S. Gubernat and Z. Lopicic, Biomass Convers. Biorefin., 15, 99 (2025); https://doi.org/10.1007/s13399-023-05264-9
  28. S.A. Jasim, I. Patra, M.J.C. Opulencia, K. Hachem, R.M.R. Parra, M.J. Ansari, A.T. Jalil, M.E. Al-Gazally, M. Naderifar, M. Khatami and R. Akhavan-Sigari, Nanotechnol. Rev., 11, 2483 (2022); https://doi.org/10.1515/ntrev-2022-0143
  29. R. Skov, R. Smyth, A. Larsen, A. Bolmstrom, A. Karlsson, K. Mills, N. Frimodt-Moller and G. Kahlmeter, J. Clin. Microbiol., 44, 4395 (2006); https://doi.org/10.1128/JCM.01411-06
  30. H. Fujikawa and M. Matsushita, J. Phys. Soc. Jpn., 58, 3875 (1989); https://doi.org/10.1143/JPSJ.58.3875
  31. S.A. Waksman, Science, 118, 259 (1953); https://doi.org/10.1126/science.118.3062.259
  32. S. Kalia, V. Dhiman and N. Prasad, Mater. Chem. Phys., 334, 130417 (2025); https://doi.org/10.1016/j.matchemphys.2025.130417
  33. K. Atacan, M. Özacar and M. Özacar, Int. J. Biol. Macromol., 109, 720 (2018); https://doi.org/10.1016/j.ijbiomac.2017.12.066
  34. İ. Gulcin and S.H. Alwasel, Processes, 11, 2248 (2023); https://doi.org/10.3390/pr11082248
  35. S.B. Kedare and R. Singh, J. Food Sci. Technol., 48, 412 (2011); https://doi.org/10.1007/s13197-011-0251-1
  36. M.C. Foti, J. Agric. Food Chem., 63, 8765 (2015); https://doi.org/10.1021/acs.jafc.5b03839
  37. M. Hashemi, S. Abolghasemi, F. Rahimi, S. Rajabi and A. Nasiri, J. Environ. Manage., 373, 123978 (2025); https://doi.org/10.1016/j.jenvman.2024.123978
  38. S. Rajabi, Z. Derakhshan, S. Maleky, A. Nasiri, B. Ahmadi, M. Feilizadeh, A. Mohammadpour, M.R. Samaei and M. Hashemi, Int. J. Biol. Macromol., 286, 138375 (2025); https://doi.org/10.1016/j.ijbiomac.2024.138375
  39. E.J. Abdelrazek, A.A. Gahlan, G.A. Gouda and A.S. Ahmed, Sci. Rep., 15, 4123 (2025); https://doi.org/10.1038/s41598-025-86209-2
  40. M. Sangeetha, S. Ambika, D. Madhan and S. Vadivel, J. Mater. Sci. Mater. Electron., 35, 368 (2024); https://doi.org/10.1007/s10854-024-12076-8
  41. M. Pourshaban-Mazandarani and A. Nasiri, J. Polym. Environ., 32, 5713 (2024); https://doi.org/10.1007/s10924-024-03340-3
  42. Z. Zhu, X. Li, Q. Zhao, Y. Li, C. Sun and Y. Cao, Mater. Res. Bull., 48, 2927 (2013); https://doi.org/10.1016/j.materresbull.2013.04.042
  43. F. Mohammed A. Alzahran, J. Arshad, M.S. Al-Buriahi, Z.A. Alrowaili and S. Munir, J. Taibah Univ. Sci., 17, 2209676 (2023); https://doi.org/10.1080/16583655.2023.2209676
  44. B. Surendra, J. Sci. Adv. Mater. Devices, 3, 44 (2018); https://doi.org/10.1016/j.jsamd.2018.01.005
  45. N. Kahzad and A. Salehzadeh, Biol. Trace Elem. Res., 198, 359 (2020); https://doi.org/10.1007/s12011-020-02055-5
  46. M. Giridhar, B. Manjunath, B. Surendra, K. Harish, S. Prashantha, T. Kiran, B. Uma and H.A. Murthy, Sci. Rep., 13, 17821 (2023); https://doi.org/10.1038/s41598-023-45029-y
  47. S. Malvilayila, M. Fukateb, Int. J. Environ. Sci., 11, 660 (2025); https://doi.org/10.64252/g2eztw05
  48. A.R. Chavan, S.B. Bhosale, S.B. Somvanshi, P.P. Khirade, J. Sol-Gel Sci. Technol., 116, 434 (2025); https://doi.org/10.1007/s10971-025-06730-8
  49. A. Pawar, B. Patil, S. Bhongale, A. Salunkhe, S. Kumar and T. Shinde, Indian J. Phys. Proc. Indian Assoc. Cultiv. Sci., 96, 3161 (2022); https://doi.org/10.1007/s12648-021-02245-4
  50. S.M. Rathod, A.R. Chavan, S.S. Jadhav, K.M. Batoo, M. Hadi and E.H. Raslan, Chem. Phys. Lett., 765, 138308 (2021); https://doi.org/10.1016/j.cplett.2020.138308
  51. K.H. Musa, A. Abdullah and A. Al-Haiqi, Food Chem., 194, 705 (2016); https://doi.org/10.1016/j.foodchem.2015.08.038
  52. N. Nenadis and M. Tsimidou, J. Am. Oil Chem. Soc., 79, 1191 (2002); https://doi.org/10.1007/s11746-002-0626-z
Read More
Author biographies is not available.
Crossref
Scopus
Google Scholar
Europe PMC
Download
PDF
Statistic
Read Counter : 1 Download : 0
PlumX