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Free Phytosterol Analysis and Characterization in Tobacco by Ultra Performance Liquid Chromatography-Atmospheric Pressure Chemical Ionization-Mass Spectrometry
Corresponding Author(s) : Yuan-Xing Duan
Asian Journal of Chemistry,
Vol. 26 No. 21 (2014): Vol 26 Issue 21
Abstract
This paper presents that the ultra-performance liquid chromatographic atmospheric pressure chemical ionization mass spectrometer as an efficient method for the identification and quantification of phytosterols in tobacco. The sample preparation consisted of extraction by methanol solution, separated on an Acquity UPLC BEH C18 column with a gradient of methanol/water at a flow of 0.4 mL/min. The determination was performed in selective ion monitoring mode. The quality parameter of the developed method was established using 6-ketocholestanol as internal standard. The limits of quantification of phytosterols varied from 0.28 to 2.21 μg/g. The intra-day and inter-day determination precision for 5 free phytosterols were less than 10 % in relative standard deviations and their recoveries were located in the range of 90.5-110.8 %. The generally and applicability of this improved method for analyzing phytosterols in tobacco were validated after a series of comparison were done. Compared with traditional methods, this method not only simplified procedures, but also save time and solvent.
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- W.-H. Liu, B. Ding, X.-M. Ruan, H.-T. Xu, J. Yang and S.-M. Liu, J. Chromatogr. A, 1163, 304 (2007); doi:10.1016/j.chroma.2007.06.043.
- I. Schmeltz, A. Depaolis and D. Hoffmann, Beitr. Tabakforsch, 8, 211 (1975).
- R.L. Stedman, Chem. Rev., 68, 153 (1968); doi:10.1021/cr60252a002.
- N.V. Kovganko and Z.N. Kashkan, Chem. Nat. Comp., 35, 479 (1999); doi:10.1007/BF02323277.
- A.I. Schepartz, J.J. Ellington and W.S. Schlotzhauer, Tobacco Sci., 24, 55 (1980).
- D. Hoffmann, I. Schmeltz, S.S. Hecht and E.L. Wynder, in eds: H. Gelboin and P.O.P. Ts'o, Polycyclic Hydrocarbons and Cancer, pp. 85-117 (1978).
- W.S. Scholtzhauer, R.F. Severson, O.T. Chortyk, R.F. Arrendale and H.C. Higman, J. Agric. Food Chem., 24, 992 (1976); doi:10.1021/jf60207a030.
- M.J. Lagarda, G. Garcia-Llatas and R. Farré, J. Pharm. Biomed. Anal., 41, 1486 (2006); doi:10.1016/j.jpba.2006.02.052.
- R.B. Jäpelt, D. Silvestro, J. Smedsgaard, P.E. Jensen and J. Jakobsen, Food Chem., 129, 217 (2011); doi:10.1016/j.foodchem.2011.04.029.
- B. Lu, Y. Zhang, X. Wu and J. Shi, Anal. Chim. Acta, 588, 50 (2007); doi:10.1016/j.aca.2007.01.067.
- M. Careri, L. Elviri and A. Mangia, J. Chromatogr. A, 935, 249 (2001); doi:10.1016/S0021-9673(01)01079-2.
References
W.-H. Liu, B. Ding, X.-M. Ruan, H.-T. Xu, J. Yang and S.-M. Liu, J. Chromatogr. A, 1163, 304 (2007); doi:10.1016/j.chroma.2007.06.043.
I. Schmeltz, A. Depaolis and D. Hoffmann, Beitr. Tabakforsch, 8, 211 (1975).
R.L. Stedman, Chem. Rev., 68, 153 (1968); doi:10.1021/cr60252a002.
N.V. Kovganko and Z.N. Kashkan, Chem. Nat. Comp., 35, 479 (1999); doi:10.1007/BF02323277.
A.I. Schepartz, J.J. Ellington and W.S. Schlotzhauer, Tobacco Sci., 24, 55 (1980).
D. Hoffmann, I. Schmeltz, S.S. Hecht and E.L. Wynder, in eds: H. Gelboin and P.O.P. Ts'o, Polycyclic Hydrocarbons and Cancer, pp. 85-117 (1978).
W.S. Scholtzhauer, R.F. Severson, O.T. Chortyk, R.F. Arrendale and H.C. Higman, J. Agric. Food Chem., 24, 992 (1976); doi:10.1021/jf60207a030.
M.J. Lagarda, G. Garcia-Llatas and R. Farré, J. Pharm. Biomed. Anal., 41, 1486 (2006); doi:10.1016/j.jpba.2006.02.052.
R.B. Jäpelt, D. Silvestro, J. Smedsgaard, P.E. Jensen and J. Jakobsen, Food Chem., 129, 217 (2011); doi:10.1016/j.foodchem.2011.04.029.
B. Lu, Y. Zhang, X. Wu and J. Shi, Anal. Chim. Acta, 588, 50 (2007); doi:10.1016/j.aca.2007.01.067.
M. Careri, L. Elviri and A. Mangia, J. Chromatogr. A, 935, 249 (2001); doi:10.1016/S0021-9673(01)01079-2.