Issue

Vol. 26 No. 21 (2014): Vol 26 Issue 21

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation of Nitrification-Denitrification Fungus for Removal of Ammonium-Nitrogen

https://doi.org/10.14233/ajchem.2014.16567
Yongkang Lv
Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, Shanxi Province, P.R. China
Feilong Niu
Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, Shanxi Province, P.R. China
Yuxiang Liu
College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, Shanxi Province, P.R. China
Xiaohua Yang
Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, Shanxi Province, P.R. China

Corresponding Author(s) : Yongkang Lv

yongkanglv@163.com

Asian Journal of Chemistry, Vol. 26 No. 21 (2014): Vol 26 Issue 21

Abstract

A filamentous fungus, which exhibited an ability to remove high-strength ammonium nitrogen and had nitrification-denitrification characteristics, was isolated from the activated sludge of a cokes wastewater treatment facility and named A60. It was provisionally identified as Fusarium sp, according to its colonial morphology, microscopic photos and 26S rDNA-ITS sequences analysis. In order to optimum its ammonium removal conditions, several experiments were designed at different carbon sources, nitrogen sources, C/N ratios (mol/mol) and pH. Under optimum conditions, an initial concentration of 450 mg/L NH4+-N was removed by 94.97 %, while total nitrogen was reduced by 88.35 %, however, with a few accumulation of hydroxylamine, nitrite and nitrate during nitrification process. Ammonium or the nitrification products such as hydroxylamine, nitrite and nitrate was used as the only source of nitrogen in aerobic denitrification experiment, respectively. The results indicated that all the kinds of nitrogen source could be utilized and converted to nitrogen gas (N2) about 35-45 %. A60 performed powerful simultaneous nitrification-denitrification ability.

Keywords

Fungus High-strength ammonium Optimization of condition Nitrification Denitrification
Lv, Y., Niu, F., Liu, Y., & Yang, X. (2014). Isolation of Nitrification-Denitrification Fungus for Removal of Ammonium-Nitrogen. Asian Journal of Chemistry, 26(21), 7207–7213. https://doi.org/10.14233/ajchem.2014.16567
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Y.K. Lv, J.Y. Yang and Y.X. Liu, Asian J. Chem., 25, 7243 (2013); doi:10.14233/ajchem.2013.14525.
  2. J.K. Kim, K.J. Park, K.S. Cho, S.-W. Nam, T.-J. Park and R. Bajpai, Bioresour. Technol., 96, 1897 (2005); doi:10.1016/j.biortech.2005.01.040.
  3. J. Wang, Y.S. Pei, Z.F. Yang and C.J. Lin, Fresenius Environ. Bull., 20, 114 (2011).
  4. A.H. Stouthamer, A.P.N. de Boer, J. van der Oost and R.J.M. van Spanning, Antonie van Leeuwenhoek, 71, 33 (1997); doi:10.1023/A:1000113824961.
  5. J.J. Su, B.Y. Liu and C.Y. Liu, Appl. Microbiol., 90, 457 (2001); doi:10.1046/j.1365-2672.2001.01265.x.
  6. X.-P. Yang, S.-M. Wang, D.-W. Zhang and L.-X. Zhou, Bioresour. Technol., 102, 854 (2011); doi:10.1016/j.biortech.2010.09.007.
  7. T. Nishio, T. Yoshikura, K. Chiba and Z. Inouye, Biosci. Biotechnol. Biochem., 58, 1574 (1994); doi:10.1271/bbb.58.1574.
  8. H.S. Joo, M. Hirai and M. Shoda, Biotechnol. Lett., 27, 773 (2005); doi:10.1007/s10529-005-5634-9.
  9. P. Li, S. Zhang, Y.L. Zhang, Y. Jiang, S.L. Chen, Y.P. Zou and D.L. Liu, Environ. Sci. Technol., 29, 9 (2006).
  10. 10Y. Lu, H. Yan, Wang, S. Zhou, J. Fu and J. Zhang, J. Hazard. Mater., 165, 1091 (2009); doi:10.1016/j.jhazmat.2008.10.091.
  11. Y. Wen, C. Xu, G. Liu, Y. Chen and Q. Zhou, Frontiers Environ. Sci. Eng., 6, 140 (2012); doi:10.1007/s11783-011-0286-6.
  12. S. Tang, Q. Yang, H.T. Shang and T. Sun, Fresenius Environ. Bull., 19, 3193 (2010).
  13. G. Koch, J.R. Vander Meer, H. Siegrist and K. Egli, Water Sci. Technol., 41, 191 (2000).
  14. J.J. Xing, H. Xiao and S.H. Deng, Chinese J. Environ. Eng., 4, 1541 (2010).
  15. C. Booth, The Genus fusarium, Agricultural Press, Beijing, pp. 1-12 (1988).
  16. D.S. Arora and P.K. Gill, Bioresour. Technol., 77, 89 (2001); doi:10.1016/S0960-8524(00)00114-0.
  17. A.R. Lesley, E.W.J. Van Niel, R.A.M. Torremans and J.G. Kuenen, Appl. Environ. Microbiol., 54, 2812 (1988).
  18. Z.X. Peng, Y.Z. Peng, Z.B. Yu, X.L. Liu, X.L. Li and R.D. Wang, Frontiers Environ. Sci. Eng., 6, 884 (2012); doi:10.1007/s11783-012-0408-9.
  19. C.- Tang, P. Zheng, C.- Wang and Q. Mahmood, Bioresour. Technol., 101, 1762 (2010); doi:10.1016/j.biortech.2009.10.032.
  20. P. Klangduen and K. Jurg, Water Sci. Technol., 39, 235 (1999).
  21. L.A. Robertson and J.G. Kuenen, J. Gen. Microbiol., 129, 2847 (1983).
  22. C. Glass, J. Silverstein and J. Oh, Water Environ. Res., 69, 1086 (1997); doi:10.2175/106143097X125803.
  23. Y.X. Zhang, J.T. Zhou, J.S. Zhang and S.Z. Yuan, J. Environ. Sci. (China), 21, 568 (2009); doi:10.1016/S1001-0742(08)62309-8.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0