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A Rapid and Sensitive UPLC-MS/MS Method for the Quantification of Danshensu in Dog Plasma and Application to Pharmacokinetics Study
Corresponding Author(s) : S. Chang
Asian Journal of Chemistry,
Vol. 26 No. 21 (2014): Vol 26 Issue 21
Abstract
A sensitive, specific and rapid ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of tanshinol (DSS) in dog plasma in the present study. The analysis were separated on a C18 column (50 × 2.1 mm, 1.7 μm) and a triple-quadtriple mass spectrometry equipped with electrospray ionization (ESI) source was applied for detection. The anion exchange solid-phase extraction cartridge was employed to extract the analysis from dog plasma, with high recovery of tanshinol (> 83 %). The method was linear over the concentration ranges of 1-1000 ng/mL for tanshinol. The lower limit of quantification (LLOQ) of tanshinol was 1 ng/mL. The intra-day and inter-day relative standard deviation (RSD) were less than 15 % and the relative error (RE) were all within 15 %. Finally, the method was successfully applied to support the pharmacokinetic study after the fufang danshen tablet was orally administrated to the beagle dog.
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References
T. Lu, J.L. Yang, X.M. Gao, P. Chen, F. Du, Y. Sun, F. Wang, F. Xu, H. Shang, Y. Huang, Y. Wang, R. Wan, C. Liu, B. Zhang and C. Li, Drug Metab. Dispos., 36, 1578 (2008); doi:10.1124/dmd.108.021592.
X.J. Luo, K.S. Bi, S.Y. Zhou, Q. Wei and R. Zhang, Biomed. Chromatogr., 15, 493 (2001); doi:10.1002/bmc.105.
B.B. Chang, L. Zhang, W.W. Cao, Y. Cao, W.- Yang, Y. Wang, Y.- Chen and X.- Liu, Acta Pharmacol. Sin., 31, 638 (2010); doi:10.1038/aps.2010.27.
X.R. Guo, X.H. Chen, L. Li, Z. Shen, X. Wang, P. Zheng, F. Duan, Y. Ma and K. Bi, J. Chromatogr. B, 873, 51 (2008); doi:10.1016/j.jchromb.2008.07.039.
X.H. Li, C. Yu, Y.B. Cai, G. Liu, J. Jia and Y. Wang, J. Chromatogr. B, 820, 41 (2005); doi:10.1016/j.jchromb.2005.03.005.
Y. Liu, X.R. Li, Y.H. Li, L. Wang and M. Xue, J. Pharm. Biomed. Anal., 53, 698 (2010); doi:10.1016/j.jpba.2010.03.041.
S.P. Wang, L. Liu, L.L. Wang, P. Jiang, L. Xiang, W.-D. Zhang and R.-H. Liu, J. Chromatogr. B, 912, 75 (2013); doi:10.1016/j.jchromb.2012.11.019.