Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction Combined with High Performance Liquid Chromatography-Fluorescence Detector for Determination of Nitrite in Urine

Jinyan Ning; Jiao Zhao; Lifen Meng; Li Bi

doi:10.14233/ajchem.2014.16523

Issue

Vol. 26 No. 21 (2014): Vol 26 Issue 21

Issue Published : September 30, 2014

This work is licensed under a Creative Commons Attribution 4.0 International License.

Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction Combined with High Performance Liquid Chromatography-Fluorescence Detector for Determination of Nitrite in Urine

https://doi.org/10.14233/ajchem.2014.16523

Jinyan Ning

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, P.R. China

Jiao Zhao

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, P.R. China

Lifen Meng

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, P.R. China

Li Bi

College of Science, Kunming University of Science and Technology, Kunming 650500, Yunnan Province, P.R. China

Corresponding Author(s) : Li Bi

libzty1990@163.com, 1073994058@qq.com

Asian Journal of Chemistry, Vol. 26 No. 21 (2014): Vol 26 Issue 21
Article Published : September 30, 2014

Abstract

A vortex-assisted surfactant-enhanced emulsification microextraction (VASEME) combined with high performance liquid chromatography-fluorescence detector (HPLC-FLD) procedure was developed for the determination of trace nitrite in human urine. The method is based on the selective reaction of nitrite with o-phenylenediamine in acid media to form benzotriazole, which exhibited strong fluorescence at 568 nm with excitation at 420 nm in alkaline medium. The fluorescence of system was enhanced by hydroxypropyl-b-cyclodextrin (HP-b-CD) through complexation. The nonionic surfactant Triton X-114 was adopted as emulsifier and n-octanol as the extraction solvent and vortex-mix was applied to assist emulsification in vortex-assisted surfactant-enhanced emulsification microextraction. Under the optimal conditions: the linearity was observed in the range of 4 to 80 ng/mL and the correlation coefficient ( r) was 0.996. The relative standard deviations were below 3.7 % (n = 5). The limit of detection (LOD) based on a signal-to-noise ratio (S/N) of 3 was 0.08 ng/mL and a signal-to-noise ratio (S/N) of 10 the quantification limits (LOQ) was 0.314 ng/mL. Good recoveries (³ 85 %) were obtained when the proposed method was applied to determine nitrite in human urine samples.

Keywords

Surfactant Vortex Microextraction High performance liquid chromatography Fluorescence Nitrite Urine

Ning, J., Zhao, J., Meng, L., & Bi, L. (2014). Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction Combined with High Performance Liquid Chromatography-Fluorescence Detector for Determination of Nitrite in Urine. Asian Journal of Chemistry, 26(21), 7129–7132. https://doi.org/10.14233/ajchem.2014.16523

Download Citation

References

G.Y. Wu and M. Morris, Biochem. J., 336, 1 (1998).
M.D.I. Ehrén, M.D.H. Iversen, M.D.O. Jansson, M.D.J. Adolfsson and M.D.N.P. Wiklund, Urology, 44, 683 (1994); doi:10.1016/S0090-4295(94)80206-8.
E.M. Fock, V.T. Bachteeva, E.A. Lavrova, S.D. Nikolaeva and R.G. Parnova, Cell Tissue Biol., 2, 516 (2008); doi:10.1134/S1990519X0805009X.
S. Moncada, R.M.J. Palmer and E.A. Higgs, Pharmacol. Rev., 43, 109 (1991).
M. Kelm and K. Yoshida, in eds: M. Feelisch and J. Stamler, Methods in Nitric Oxide Research, John Wiley & Sons, Chichester, pp. 47-58 (1996).
J.O.N. Lundberg, S. Carlsson, L. Engstrand, E. Morcos, N.P. Wiklund and E. Weitzberg, Urology, 50, 189 (1997); doi:10.1016/S0090-4295(97)00257-4.
A. Afkhami, T. Madrakian and A. Maleki, Anal. Biochem., 347, 162 (2005); doi:10.1016/j.ab.2005.09.018.
X.F. Yue, Z.Q. Zhang and H.T. Yan, Talanta, 62, 97 (2004); doi:10.1016/S0039-9140(03)00421-1.
L. Wang, J. Chen, H. Chen, C. Zhou, B. Ling and J. Fu, J. Lumin., 131, 83 (2011); doi:10.1016/j.jlumin.2010.09.003.
Q.H. Liu, X.L. Yan, J.C. Guo, D.H. Wang, L. Li, F.Y. Yan and L.G. Chen, Spectrochim. Acta A, 73, 789 (2009); doi:10.1016/j.saa.2009.03.018.
K.J. Huang, H. Wang, Y.H. Guo, R.-L. Fan and H.-S. Zhang, Talanta, 69, 73 (2006); doi:10.1016/j.talanta.2005.08.062.
H. Li, C.J. Meininger and G. Wu, J. Chromatogr. B Biomed. Sci. Appl., 746, 199 (2000); doi:10.1016/S0378-4347(00)00328-5.
W.S. Jobgen, S.C. Jobgen, H. Li, C.J. Meininger and G. Wu, J. Chromatogr. B, 851, 71 (2007); doi:10.1016/j.jchromb.2006.07.018.
M.D. Croitoru, J. Chromatogr. B, 911, 154 (2012); doi:10.1016/j.jchromb.2012.11.006.
J. Hsu, J. Arcot and N. Alice Lee, Food Chem., 115, 334 (2009); doi:10.1016/j.foodchem.2008.11.081.
M.I. Helaleh and T. Korenaga, J. Chromatogr. B Biomed. Sci. Appl., 744, 433 (2000); doi:10.1016/S0378-4347(00)00264-4.
D.C. Siu and A. Henshall, J. Chromatogr. A, 804, 157 (1998); doi:10.1016/S0021-9673(97)01245-4.
P. Niedzielski, I. Kurzyca and J. Siepak, Anal. Chim. Acta, 577, 220 (2006); doi:10.1016/j.aca.2006.06.057.
S. Kage, K. Kudo and N. Ikeda, J. Chromatogr. B Biomed. Sci. Appl., 742, 363 (2000); doi:10.1016/S0378-4347(00)00189-4.
D. Tsikas, R.H. Böger, S.M. Bode-Böger, F.M. Gutzki and J.C. Frölich, J. Chromatogr. B Biomed. Sci. Appl., 661, 185 (1994); doi:10.1016/0378-4347(94)00374-2.
M.C. Boyce, Electrophoresis, 22, 1447 (2001); doi:10.1002/1522-2683(200105)22:8<1447::AID-ELPS1447>3.0.CO;2-#.
L. He, K. Zhang, C. Wang, X. Luo and S. Zhang, J. Chromatogr. A, 1218, 3595 (2011); doi:10.1016/j.chroma.2011.04.014.
W. Utermahlen Jr., D. Mellini and H. Issaq, J. Liq. Chromatogr. Rel. Technol., 15, 3315 (1992); doi:10.1080/10826079208020886.
M. Zhang, D. Yuan, G. Chen, Q. Li, Z. Zhang and Y. Liang, Mikrochim. Acta, 165, 427 (2009); doi:10.1007/s00604-009-0158-y.
N. Pourreza, M.R. Fat'hi and A. Hatami, Microchem. J., 104, 22 (2012); doi:10.1016/j.microc.2012.03.026.
H. Filik, D. Giray, B. Ceylan and R. Apak, Talanta, 85, 1818 (2011); doi:10.1016/j.talanta.2011.07.052.
Y.X. Guo, Q.F. Zhang, X. Shangguang and G. Zhen, Spectrochim. Acta A, 101, 107 (2013); doi:10.1016/j.saa.2012.09.083.

References

G.Y. Wu and M. Morris, Biochem. J., 336, 1 (1998).

M.D.I. Ehrén, M.D.H. Iversen, M.D.O. Jansson, M.D.J. Adolfsson and M.D.N.P. Wiklund, Urology, 44, 683 (1994); doi:10.1016/S0090-4295(94)80206-8.

E.M. Fock, V.T. Bachteeva, E.A. Lavrova, S.D. Nikolaeva and R.G. Parnova, Cell Tissue Biol., 2, 516 (2008); doi:10.1134/S1990519X0805009X.

S. Moncada, R.M.J. Palmer and E.A. Higgs, Pharmacol. Rev., 43, 109 (1991).

M. Kelm and K. Yoshida, in eds: M. Feelisch and J. Stamler, Methods in Nitric Oxide Research, John Wiley & Sons, Chichester, pp. 47-58 (1996).

J.O.N. Lundberg, S. Carlsson, L. Engstrand, E. Morcos, N.P. Wiklund and E. Weitzberg, Urology, 50, 189 (1997); doi:10.1016/S0090-4295(97)00257-4.

A. Afkhami, T. Madrakian and A. Maleki, Anal. Biochem., 347, 162 (2005); doi:10.1016/j.ab.2005.09.018.

X.F. Yue, Z.Q. Zhang and H.T. Yan, Talanta, 62, 97 (2004); doi:10.1016/S0039-9140(03)00421-1.

L. Wang, J. Chen, H. Chen, C. Zhou, B. Ling and J. Fu, J. Lumin., 131, 83 (2011); doi:10.1016/j.jlumin.2010.09.003.

Q.H. Liu, X.L. Yan, J.C. Guo, D.H. Wang, L. Li, F.Y. Yan and L.G. Chen, Spectrochim. Acta A, 73, 789 (2009); doi:10.1016/j.saa.2009.03.018.

K.J. Huang, H. Wang, Y.H. Guo, R.-L. Fan and H.-S. Zhang, Talanta, 69, 73 (2006); doi:10.1016/j.talanta.2005.08.062.

H. Li, C.J. Meininger and G. Wu, J. Chromatogr. B Biomed. Sci. Appl., 746, 199 (2000); doi:10.1016/S0378-4347(00)00328-5.

W.S. Jobgen, S.C. Jobgen, H. Li, C.J. Meininger and G. Wu, J. Chromatogr. B, 851, 71 (2007); doi:10.1016/j.jchromb.2006.07.018.

M.D. Croitoru, J. Chromatogr. B, 911, 154 (2012); doi:10.1016/j.jchromb.2012.11.006.

J. Hsu, J. Arcot and N. Alice Lee, Food Chem., 115, 334 (2009); doi:10.1016/j.foodchem.2008.11.081.

M.I. Helaleh and T. Korenaga, J. Chromatogr. B Biomed. Sci. Appl., 744, 433 (2000); doi:10.1016/S0378-4347(00)00264-4.

D.C. Siu and A. Henshall, J. Chromatogr. A, 804, 157 (1998); doi:10.1016/S0021-9673(97)01245-4.

P. Niedzielski, I. Kurzyca and J. Siepak, Anal. Chim. Acta, 577, 220 (2006); doi:10.1016/j.aca.2006.06.057.

S. Kage, K. Kudo and N. Ikeda, J. Chromatogr. B Biomed. Sci. Appl., 742, 363 (2000); doi:10.1016/S0378-4347(00)00189-4.

D. Tsikas, R.H. Böger, S.M. Bode-Böger, F.M. Gutzki and J.C. Frölich, J. Chromatogr. B Biomed. Sci. Appl., 661, 185 (1994); doi:10.1016/0378-4347(94)00374-2.

M.C. Boyce, Electrophoresis, 22, 1447 (2001); doi:10.1002/1522-2683(200105)22:8<1447::AID-ELPS1447>3.0.CO;2-#.

L. He, K. Zhang, C. Wang, X. Luo and S. Zhang, J. Chromatogr. A, 1218, 3595 (2011); doi:10.1016/j.chroma.2011.04.014.

W. Utermahlen Jr., D. Mellini and H. Issaq, J. Liq. Chromatogr. Rel. Technol., 15, 3315 (1992); doi:10.1080/10826079208020886.

M. Zhang, D. Yuan, G. Chen, Q. Li, Z. Zhang and Y. Liang, Mikrochim. Acta, 165, 427 (2009); doi:10.1007/s00604-009-0158-y.

N. Pourreza, M.R. Fat'hi and A. Hatami, Microchem. J., 104, 22 (2012); doi:10.1016/j.microc.2012.03.026.

H. Filik, D. Giray, B. Ceylan and R. Apak, Talanta, 85, 1818 (2011); doi:10.1016/j.talanta.2011.07.052.

Y.X. Guo, Q.F. Zhang, X. Shangguang and G. Zhen, Spectrochim. Acta A, 101, 107 (2013); doi:10.1016/j.saa.2012.09.083.

Issue

Vol. 26 No. 21 (2014): Vol 26 Issue 21

Vortex-Assisted Surfactant-Enhanced Emulsification Microextraction Combined with High Performance Liquid Chromatography-Fluorescence Detector for Determination of Nitrite in Urine

Corresponding Author(s) : Li Bi

Abstract

Keywords

Full Article

Download Citation

References

Table Of Contents