Issue

Vol. 26 No. 21 (2014): Vol 26 Issue 21

Copyright (c) 2014 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Antitumor Activity in vitro by 9-Oxo-10,11-dehydroageraphorone Extracted from Eupatorium adenophorum

https://doi.org/10.14233/ajchem.2014.16696
Fei Liao
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China ; Qiandongnan Prefectural Center for Disease Control and Prevention of Guizhou Province, Kaili 556000, P.R. China; These authors contributed equally to this work and should be considered as first authors
Yanchun Hu
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China ; These authors contributed equally to this work and should be considered as first authors
Lei Wu
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Hui Tan
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Quan Mo
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Biao Luo
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Yajun He
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Junliang Deng
Key laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, Ya an 625014, P.R. China
Yahui Wei
Key Laboratory of Resource Biology and Biotechnology in Western China, School of Life Science, Northwest University, Xi'an 710069, P.R. China

Corresponding Author(s) : Yanchun Hu

yanchunhu@126.com

Asian Journal of Chemistry, Vol. 26 No. 21 (2014): Vol 26 Issue 21

Abstract

Ultrasonic-methanol extraction was used to extract cadenine sesquiterpene from Eupatorium adenophorum and then the crude 9-oxo-10,11-dehydroageraphorone (euptox A) was purified by column chromatography and XAD-2 Macroporous Resin. 4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the cytotoxicity of euptox A to human lung cancer A549 cells, Hela cells and Hep-2 cells in vitro. The results suggest that euptox A had significant antitumor activity against the three tumor cell lines in vitro in a dose-dependent manner. When the concentration of euptox A was at 500 μg/mL, the percent inhibition of human lung cancer A549 cells, Hela cells and Hep-2 cells were 76.42, 68.30 and 79.05 %, respectively. The 50 % inhibitory concentration (IC50) of euptox A for the three tumor cell lines were 369, 401 and 427 μg/mL (A549, Hela and Hep-2 cells, respectively). The results suggest it's better than the control of the 5-FU and euptox A may be considered as a potential candidate for developing a novel low toxicity antitumor agent.

Keywords

Euptox A Antitumor activity Eupatorium adenophorum
Liao, F., Hu, Y., Wu, L., Tan, H., Mo, Q., Luo, B., … Wei, Y. (2014). Antitumor Activity in vitro by 9-Oxo-10,11-dehydroageraphorone Extracted from Eupatorium adenophorum. Asian Journal of Chemistry, 26(21), 7321–7323. https://doi.org/10.14233/ajchem.2014.16696
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Z.J. Lu and K.P. Ma, Weed Sci., 54, 1068 (2006); doi:10.1614/WS-06-040R1.1.
  2. A. Sahoo, B. Singh and O.P. Sharma, Livest. Sci., 136, 175 (2011); doi:10.1016/j.livsci.2010.08.019.
  3. K. Chakravarty, T. Mazumder and S.N. Chatterjee, Evid.-Based Complem. Altern. Med., Article ID 471074 (2011); doi:10.1093/ecam/neq1033.
  4. R.C. Padalia, D.S. Bisht, S.C. Joshi and C.S. Mathela, J. Essent. Oil. Res., 21, 522 (2009); doi:10.1080/10412905.2009.9700234.
  5. T. Kikuchi, K. Watanabe, Y. Tochigi, A. Yamamoto, M. Fukatsu, Y. Ezaki, R. Tanaka and T. Akihisa, Chem. Biodivers., 9, 1500 (2012); doi:10.1002/cbdv.201200111.
  6. S.V. Amand, A. Langenfeld, A. Blond, J. Dupont, B. Nay and S. Prado, J. Nat. Prod., 75, 798 (2012); doi:10.1021/np2009913.
  7. L. Trzoss, J. Xu, M.H. Lacoske, W.C. Mobley and E.A. Theodorakis, Chem. Eur. J., 19, 6398 (2013); doi:10.1002/chem.201300198.
  8. I. Montenegro, L. Pino, E. Werner, A. Madrid, L. Espinoza, L. Moreno, J. Villena and M. Cuellar, Molecules, 18, 4192 (2013); doi:10.3390/molecules18044192.
  9. A.A. Wube, F. Bucar, S. Gibbons, K. Asres, L. Rattray and S.L. Croft, Phytother. Res., 24, 1468 (2010); doi:10.1002/ptr.3126.
  10. R. Bhardwaj, A. Singh, O.P. Sharma, R.K. Dawra, N.P. Kurade and S.B. Mahato, J. Biochem. Mol. Toxicol., 15, 279 (2001); doi:10.1002/jbt.10001.
  11. X.Z. Zhu, J.T. Zhang and K.P. Ma, PloS one, 6, 253 (2011).
  12. F. Liao, Y.C. Hu, H. Tan, L. Wu, Y. Huang, Y.F. Wang, Q. Mo and Y.H. Wei, Exp. Parasitol., 140, 8 (2014); doi:10.1016/j.exppara.2014.02.009.
  13. L. He, J. Yang, A.C. Cao, Y.M. Liu, Y. An and J.-G. Shi, Chin. J. Chem., 24, 1375 (2006); doi:10.1002/cjoc.200690256.
  14. L. Ho, D. Hoefel, C.P. Saint and G. Newcombe, Water Res., 41, 4685 (2007); doi:10.1016/j.watres.2007.06.057.
  15. A.T. Taher, H.H. Georgey and H.I. El-Subbagh, Eur. J. Med. Chem., 47, 445 (2012); doi:10.1016/j.ejmech.2011.11.013.
  16. L. Zhang, S.R. Koyyalamudi, S.C. Jeong, N. Reddy, P.T. Smith, R. Ananthan and T. Longvah, Int. J. Biol. Macromol., 51, 1057 (2012); doi:10.1016/j.ijbiomac.2012.08.019.
  17. I. Khan, M. Nisar, M. Ahmad, H. Shah, Z. Iqbal, M. Saeed, S.M.A. Halimi, W.A. Kaleem, M. Qayum, A. Aman and S.M. Abdullah, Fitoterapia, 82, 276 (2011); doi:10.1016/j.fitote.2010.10.011.
  18. Q. Xu, Y. Liu, S. Su, W. Li, C. Chen and Y. Wu, Biomaterials, 33, 1627 (2012); doi:10.1016/j.biomaterials.2011.11.012.
  19. D.P. Fulzele and R.K. Satdive, Fitoterapia, 76, 643 (2005); doi:10.1016/j.fitote.2005.07.005.
  20. B. Ramesha, H. Suma, U. Senthilkumar, V. Priti, G. Ravikanth, R. Vasudeva, T. Kumar, K. Ganeshaiah and R. U. Shaanker, Phytomedicine, 20, 521 (2013); doi:10.1016/j.phymed.2012.12.003.
  21. L. Qin, M. Xue, W. Wang, R. Zhu, S. Wang, J. Sun, R. Zhang and X. Sun, J. Essent. Oil Res., 388, 223 (2010).
  22. J.Q. Zhang, Y.M. Li, T. Liu, W.T. He, Y.T. Chen, X.H. Chen, X. Li, W.C. Zhou, J.F. Yi, Z.J. Ren and J. World, Gastroenterol., 16, 4281 (2010).
  23. T. Zhang, Y. Zheng, Q. Peng, X. Cao, T. Gong and Z. Zhang, Int. J. Nanomed., 8, 1185 (2013); doi:10.2147/IJN.S41775.
  24. F. Li, F. Wang, F. Yu, Y. Fang, Z. Xin, F. Yang, J. Xu, L. Zhao and Q. Hu, Food Chem., 111, 165 (2008); doi:10.1016/j.foodchem.2008.03.057.
  25. Q. Yan, J. Yang, H. Li, A. Cao, Q. Chen, Y. Wen and L. He, J. Being Norm, Univ. Nat. (Sci. Ed.), 42, 70 (2006).
  26. Y. Sato, S. Itagaki, T. Kurokawa, J. Ogura, M. Kobayashi, T. Hirano, M. Sugawara and K. Iseki, Int. J. Pharm., 403, 136 (2011); doi:10.1016/j.ijpharm.2010.09.035.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 1 Download : 0