Comparison of Physico-Chemical and Antioxidant Activity of  Sargassum henslowianum J. Agardh by Different Extraction Methods

N.T. Viet; L.T. Le; N.N. Quy; N.M. Tien; T.C. Thai; T.K.N. Tran

doi:10.14233/ajchem.2025.33580

Issue

Vol. 37 No. 7 (2025): Vol 37 Issue 7, 2025

Issue Published : 30 June 2025

This work is licensed under a Creative Commons Attribution 4.0 International License.

Comparison of Physico-Chemical and Antioxidant Activity of Sargassum henslowianum J. Agardh by Different Extraction Methods

https://doi.org/10.14233/ajchem.2025.33580

N.T. Viet

Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam; NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

https://orcid.org/0000-0001-8489-7799

L.T. Le

Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam

N.N. Quy

Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam

https://orcid.org/0000-0003-3865-6518

N.M. Tien

Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City 700000, Vietnam

https://orcid.org/0009-0001-4769-8114

T.C. Thai

Reseach and Development Institute of Advanced Agribilogy, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

https://orcid.org/0009-0002-6071-8201

T.K.N. Tran

Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam; NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

https://orcid.org/0000-0002-1041-4851

Corresponding Author(s) : N.T. Viet

ntviet@ntt.edu.vn

Asian Journal of Chemistry, Vol. 37 No. 7 (2025): Vol 37 Issue 7, 2025
Article Published : 30 June 2025

Abstract

Sargassum henslowianum is a seaweed that shows promise for extracting valuable compounds used in various fields. This study focused on the extraction of nutrients from S. henslowianum to identify biologically active compounds and assess acetylcholinesterase inhibitory activity in different extracts. The seaweed was extracted using water and EDTA and the extracts were analyzed for active compounds and AChE inhibitory activity using Ellman’s spectrophotometric method. The results indicated that the water extract had higher levels of polyphenols, flavonoids and antioxidant activity, with IC₅₀ values of 1977.54 µg mL^–1 (DPPH) and 68.82 µg mL^–1 (ABTS). The water extract also showed a MIC of 2.813 mg mL^–1 against E. coli and S. aureus, while the EDTA extract had a MIC of 5.625 mg mL^–1. This study highlights the potential of seaweed as a source of acetylcholinesterase enzyme inhibitors. Extracting high-quality compounds from seaweed could lead to new opportunities in the pharmaceutical and cosmetic industries, supporting sustainable development in the marine sector.

Keywords

Sargassum henslowianum Physico-chemical Antioxidant Antibacterial Extraction

(1)

Viet, N.; Le, L.; Quy, N.; Tien, N.; Thai, T.; Tran, T. Comparison of Physico-Chemical and Antioxidant Activity of Sargassum Henslowianum J. Agardh by Different Extraction Methods. ajc 2025, 37, 1720-1726.

Download Citation

References

B. Minh Ly, N. Quoc Buu, N. Duy Nhut, P. Duc Thinh and T. Thi Thanh Van, ASEAN J. Sci. Technol. Dev., 22, 371 (2017); https://doi.org/10.29037/ajstd.173.
M.I. Rushdi, I.A.M. Abdel-Rahman, H. Saber, E.Z. Attia, W.M. Abdelraheem, H.A. Madkour, H.M. Hassan, A.H. Elmaidomy and U.R. Abdelmohsen, RSC Adv., 10, 24951 (2020); https://doi.org/10.1039/D0RA03576A
S. Al-Hashdy, S. Raweh, A. El-Shaibany, A. Humaid and M. El-Aasser, Int. J. Res. Dev. Pharm. Life Sci., 8, 2 (2022).
J.K. Patra, S.K. Rath, K. Jena, V.K. Rathod and H. Thatoi, Turk. J. Biol., 32, 119 (2008).
A.E. Abd El-Wahab, D.A. Ghareeb, E.E.M. Sarhan, M.M. Abu-Serie and M.A. El Demellawy, BMC Complement. Altern. Med., 13, 218 (2013); https://doi.org/10.1186/1472-6882-13-218
A. Husni, N. Izmi, F.Z. Ayunani, A. Kartini, N. Husnayain and A. Isnansetyo, Int. J. Food Sci., 2022, 1 (2022); https://doi.org/10.1155/2022/3689724
A.A. Ageeli and S.F. Mohamed, Chemistry, 5, 2756 (2023); https://doi.org/10.3390/chemistry5040176
B. Luo, Z. Wang, J. Chen, X. Chen, J. Li, Y. Li, R. Li, X. Liu, B. Song, K.L. Cheong and S. Zhong, Molecules, 28, 2610 (2023); https://doi.org/10.3390/molecules28062610
Q.-L. Sun, Y. Li, L.-Q. Ni, Y.-X. Li, Y.-S. Cui, S.-L. Jiang, E.-Y. Xie, J. Du, F. Deng and C.-X. Dong, Carbohydr. Polym., 229, 115487 (2020); https://doi.org/10.1016/j.carbpol.2019.115487
D. Al-hashdy, S. Raweh, A. El-shaibany, A. Humaid and M. El-aasser, Int. J. Res. Dev. Pharm. Life Sci., 8, (2022); https://doi.org/10.4172/2278-0238.1000130
M. Sanjivkumar, S.T. Selvan, B. Velramar, K. Nagajothi, S.S.M. Sophia and A. Parameswari, Biotherapeutic Potential and Properties of Seaweeds; In: Whole-Cell Biocatalysis. Apple Academic Press, pp. 199–220 (2024).
M.D.N. Meinita, D. Harwanto and J.-S. Choi, Appl. Sci., 12, 2638 (2022); https://doi.org/10.3390/app12052638
J. Liu, S.-Y. Wu, L. Chen, Q.-J. Li, Y.-Z. Shen, L. Jin, X. Zhang, P.-C. Chen, M.-J. Wu, J. Choi and H.-B. Tong, Int. J. Biol. Macromol., 155, 1385 (2020); https://doi.org/10.1016/j.ijbiomac.2019.11.113
P.L. Thao My, V.V. Sung, T.D. Dat, H.M. Nam, M.T. Phong and N.H. Hieu, ChemistrySelect, 5, 4371 (2020); https://doi.org/10.1002/slct.201903818
N.M.S. Moubayed, H.J. Al Houri, M.M. Al Khulaifi and D.A. Al Farraj, Saudi J. Biol. Sci., 24, 162 (2017); https://doi.org/10.1016/j.sjbs.2016.05.018
S.J. Lim and W.M.W. Aida, Extraction of Sulfated Polysaccharides (Fucoidan) from Brown Seaweed; In: Seaweed Polysaccharides. Elsevier, pp. 27–46 (2017).
K. Rafiñska, P. Pomastowski, J. Rudnicka, A. Krakowska, A. Maruska, M. Narkute and B. Buszewski, Food Chem., 289, 16 (2019); https://doi.org/10.1016/j.foodchem.2019.03.025
M. Heinrich, J. Mah and V. Amirkia, Molecules, 26, 1836 (2021); https://doi.org/10.3390/molecules26071836
B. Tan, X. Wu, J. Yu and Z. Chen, Molecules, 27, 3956 (2022); https://doi.org/10.3390/molecules27123956
R. Lailatussifa, A. Husni and A.E. Nugroho, Food Sci. Biotechnol., 25, 589 (2016); https://doi.org/10.1007/s10068-016-0082-y
M. Rudrapal, S.J. Khairnar, J. Khan, A.B. Dukhyil, M.A. Ansari, M.N. Alomary, F.M. Alshabrmi, S. Palai, P.K. Deb and R. Devi, Front. Pharmacol., 13, 806470 (2022); https://doi.org/10.3389/fphar.2022.806470
L. Wang and C.L. Weller, Trends Food Sci. Technol., 17, 300 (2006); https://doi.org/10.1016/j.tifs.2005.12.004
Y. Velioglu, G. Mazza, L. Gao and B.D. Oomah, J. Agric. Food Chem., 46, 4113 (1998); https://doi.org/10.1021/jf9801973
G. Marucci, M. Buccioni, D.D. Ben, C. Lambertucci, R. Volpini and F. Amenta, Neuropharmacology, 190, 108352 (2021); https://doi.org/10.1016/j.neuropharm.2020.108352

References

B. Minh Ly, N. Quoc Buu, N. Duy Nhut, P. Duc Thinh and T. Thi Thanh Van, ASEAN J. Sci. Technol. Dev., 22, 371 (2017); https://doi.org/10.29037/ajstd.173.

M.I. Rushdi, I.A.M. Abdel-Rahman, H. Saber, E.Z. Attia, W.M. Abdelraheem, H.A. Madkour, H.M. Hassan, A.H. Elmaidomy and U.R. Abdelmohsen, RSC Adv., 10, 24951 (2020); https://doi.org/10.1039/D0RA03576A

S. Al-Hashdy, S. Raweh, A. El-Shaibany, A. Humaid and M. El-Aasser, Int. J. Res. Dev. Pharm. Life Sci., 8, 2 (2022).

J.K. Patra, S.K. Rath, K. Jena, V.K. Rathod and H. Thatoi, Turk. J. Biol., 32, 119 (2008).

A.E. Abd El-Wahab, D.A. Ghareeb, E.E.M. Sarhan, M.M. Abu-Serie and M.A. El Demellawy, BMC Complement. Altern. Med., 13, 218 (2013); https://doi.org/10.1186/1472-6882-13-218

A. Husni, N. Izmi, F.Z. Ayunani, A. Kartini, N. Husnayain and A. Isnansetyo, Int. J. Food Sci., 2022, 1 (2022); https://doi.org/10.1155/2022/3689724

A.A. Ageeli and S.F. Mohamed, Chemistry, 5, 2756 (2023); https://doi.org/10.3390/chemistry5040176

B. Luo, Z. Wang, J. Chen, X. Chen, J. Li, Y. Li, R. Li, X. Liu, B. Song, K.L. Cheong and S. Zhong, Molecules, 28, 2610 (2023); https://doi.org/10.3390/molecules28062610

Q.-L. Sun, Y. Li, L.-Q. Ni, Y.-X. Li, Y.-S. Cui, S.-L. Jiang, E.-Y. Xie, J. Du, F. Deng and C.-X. Dong, Carbohydr. Polym., 229, 115487 (2020); https://doi.org/10.1016/j.carbpol.2019.115487

D. Al-hashdy, S. Raweh, A. El-shaibany, A. Humaid and M. El-aasser, Int. J. Res. Dev. Pharm. Life Sci., 8, (2022); https://doi.org/10.4172/2278-0238.1000130

M. Sanjivkumar, S.T. Selvan, B. Velramar, K. Nagajothi, S.S.M. Sophia and A. Parameswari, Biotherapeutic Potential and Properties of Seaweeds; In: Whole-Cell Biocatalysis. Apple Academic Press, pp. 199–220 (2024).

M.D.N. Meinita, D. Harwanto and J.-S. Choi, Appl. Sci., 12, 2638 (2022); https://doi.org/10.3390/app12052638

J. Liu, S.-Y. Wu, L. Chen, Q.-J. Li, Y.-Z. Shen, L. Jin, X. Zhang, P.-C. Chen, M.-J. Wu, J. Choi and H.-B. Tong, Int. J. Biol. Macromol., 155, 1385 (2020); https://doi.org/10.1016/j.ijbiomac.2019.11.113

P.L. Thao My, V.V. Sung, T.D. Dat, H.M. Nam, M.T. Phong and N.H. Hieu, ChemistrySelect, 5, 4371 (2020); https://doi.org/10.1002/slct.201903818

N.M.S. Moubayed, H.J. Al Houri, M.M. Al Khulaifi and D.A. Al Farraj, Saudi J. Biol. Sci., 24, 162 (2017); https://doi.org/10.1016/j.sjbs.2016.05.018

S.J. Lim and W.M.W. Aida, Extraction of Sulfated Polysaccharides (Fucoidan) from Brown Seaweed; In: Seaweed Polysaccharides. Elsevier, pp. 27–46 (2017).

K. Rafiñska, P. Pomastowski, J. Rudnicka, A. Krakowska, A. Maruska, M. Narkute and B. Buszewski, Food Chem., 289, 16 (2019); https://doi.org/10.1016/j.foodchem.2019.03.025

M. Heinrich, J. Mah and V. Amirkia, Molecules, 26, 1836 (2021); https://doi.org/10.3390/molecules26071836

B. Tan, X. Wu, J. Yu and Z. Chen, Molecules, 27, 3956 (2022); https://doi.org/10.3390/molecules27123956

R. Lailatussifa, A. Husni and A.E. Nugroho, Food Sci. Biotechnol., 25, 589 (2016); https://doi.org/10.1007/s10068-016-0082-y

M. Rudrapal, S.J. Khairnar, J. Khan, A.B. Dukhyil, M.A. Ansari, M.N. Alomary, F.M. Alshabrmi, S. Palai, P.K. Deb and R. Devi, Front. Pharmacol., 13, 806470 (2022); https://doi.org/10.3389/fphar.2022.806470

L. Wang and C.L. Weller, Trends Food Sci. Technol., 17, 300 (2006); https://doi.org/10.1016/j.tifs.2005.12.004

Y. Velioglu, G. Mazza, L. Gao and B.D. Oomah, J. Agric. Food Chem., 46, 4113 (1998); https://doi.org/10.1021/jf9801973

G. Marucci, M. Buccioni, D.D. Ben, C. Lambertucci, R. Volpini and F. Amenta, Neuropharmacology, 190, 108352 (2021); https://doi.org/10.1016/j.neuropharm.2020.108352

Author biographies is not available.