Isolation of Quercetin and Anticonvulsant Properties of Ethanol Extract  Derived from Stem of Tragia involucrata Linn. (Euphorbiaceae)

N. Saraswathy; A. Satish; M. Chitravel; S. Krishnaveni; S.S. Syed Abuthahir

doi:10.14233/ajchem.2025.33875

Issue

Vol. 37 No. 7 (2025): Vol 37 Issue 7, 2025

Issue Published : 30 June 2025

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation of Quercetin and Anticonvulsant Properties of Ethanol Extract Derived from Stem of Tragia involucrata Linn. (Euphorbiaceae)

https://doi.org/10.14233/ajchem.2025.33875

N. Saraswathy

Department of Science and Humanities, Parisutham Institute of Technology and Science, Thanjavur-613006, India

https://orcid.org/0009-0009-0980-7568

A. Satish

Department of Chemistry, St. Joseph’s College of Engineering and Technology, Thanjavur-614403, India

https://orcid.org/0009-0005-5415-6769

M. Chitravel

Department of Chemistry, St. Joseph’s College of Engineering and Technology, Thanjavur-614403, India

https://orcid.org/0009-0000-9126-3137

S. Krishnaveni

Department of Chemistry, St. Joseph’s College of Engineering and Technology, Thanjavur-614403, India

https://orcid.org/0009-0001-1379-2356

S.S. Syed Abuthahir

PG and Research Department of Chemistry, Jamal Mohamed College (Autonomous), Affiliated to Bharathidasan University, Tiruchirappalli-620020, India

https://orcid.org/0000-0002-7872-0943

Corresponding Author(s) : N. Saraswathy

saraswathyrajam@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 7 (2025): Vol 37 Issue 7, 2025
Article Published : 30 June 2025

Abstract

Indian and Sri Lankan traditional medicines make the use of Tragia involucrata Linn., a plant belonging to the Euphorbiaceae family. This study aimed to isolate the bioactive flavonoid quercetin from the ethanolic extracts of stem of T. involucrata and evaluate its anticonvulsant effects. The ethanolic extracts from the plant underwent a thorough phytochemical analysis using column chromatography and TLC techniques. The isolated chemical was examined using mass spectrometry, UV, FT-IR, ¹H NMR, ¹³C NMR, etc. The LD₅₀ of the ethanolic extract was obtained using the acute toxicity assessments. Albino rats were subjected to convulsions induced by MES and lithium pilocarpine to evaluate the anticonvulsant effectiveness. The ethanolic extract of the stem diminishes the maximum electroshock (MES)-induced phases of flexion, hindlimb extension, clonus and stupor during convulsions. Lithium-pilocarpine convulsions were evaluated against a diazepam group and a control group and has shown anticonvulsant activity.

Keywords

Anticonvulsant activity Ethanol extract Lithium pilocarpine Quercetin Tragia involucrata

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Saraswathy, N.; Satish, A.; Chitravel, M.; Krishnaveni, S.; Abuthahir, S. S. Isolation of Quercetin and Anticonvulsant Properties of Ethanol Extract Derived from Stem of Tragia Involucrata Linn. (Euphorbiaceae). ajc 2025, 37, 1649-1654.

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References

S. Sen, R. Chakraborty and B. De, J. Herbal Med., 1, 67 (2011); https://doi.org/10.1016/j.hermed.2011.11.001
, S. Saggar, P.A. Mir, N. Kumar, A. Chawla, J. Uppal, Shilpa and A. Kaur, Pharmacogn. Res., 14, 107 (2022); https://doi.org/10.5530/pres.14.2.15
R.P. Samy, P. Gopalakrishnakone, P. Houghton and S. Ignacimuthu, J. Ethnopharmacol., 107, 99 (2006); https://doi.org/10.1016/j.jep.2006.02.020
M.S. Pallie, P.K. Perera, N. Kumarasinghe, M. Arawwawala and C.L. Goonasekara, Evid. Based Complement Alternat Med., 2020, 8848676 (2020); https://doi.org/10.1155/2020/8848676
B. Saraceno, G. Avanzini and P. Lee, ‘Atlas: Epilepsy care in the world’, WHO, (2005).
C.R. Newton and H.H. Garcia, Lancet, 380, 1193 (2012); https://doi.org/10.1016/S0140-6736(12)61381-6
B.S. Reddy, N.R. Rao, K. Vijeepallam and V. Pandy, Afr. J. Tradit. Complement Altern. Med., 14, 105 (2017); https://doi.org/10.21010/ajtcam.v14i3.11
S. Vigneshwaran, K. Maharani, P. Sivasakthi, P.S. Selvan, S.D. Saraswathy and E.S. Priya, Inflammopharmacology, 31, 967 (2023); https://doi.org/10.1007/s10787-023-01154-8
G.A. Ayoola, O.O. Johnson, J. Aderounmu, L. Raji, R.B. Aremu, S. Bankole, Trop. J. Phytochem. Pharm. Sci., 3, 448 (2024); https://doi.org/10.26538/tjpps/v3i9.4
J.B. Harbone, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, New York: Chapman and Hall Publishers, pp. 49–188 (1973).
M.K. Shelar, M.J. Patil, S. Bhujbal and R.B. Chaudhari, Future J. Pharm. Sci., 4, 215 (2018); https://doi.org/10.1016/j.fjps.2018.06.002
H.O. Edeoga, D.E. Okwu and B.O. Mbaebie, Afr. J. Biotechnol., 4, 685 (2005); https://doi.org/10.5897/AJB2005.000-3127
E. Bienvenu, G.J. Amabeoku, P.K. Eagles, G. Scott and E.P. Springfield, Phytomedicine, 9, 217 (2002); https://doi.org/10.1078/0944-7113-00103
S. Patel, B.S. Meldrum and A. Fine, Behav. Brain Res., 31, 165 (1988); https://doi.org/10.1016/0166-4328(88)90019-8
V. Velu, S. Banerjee, V. Radhakrishnan, G. Gupta, D.K. Chellappan, N.K. Fuloria, S. Fuloria, M. Mehta, K. Dua and H. Malipeddi, Antiinflamm. Antiallergy Agents Med. Chem., 20, 308 (2021); https://doi.org/10.2174/1871523020666210126144506
M. Menon and L. Varghese, J. Appl. Biol. Biotechnol., 11, 84 (2023); https://doi.org/10.7324/JABB.2023.87672
P. Singh, I. Singh and R.K. Goel, Int. J. Pharm. Pharm. Sci., 6, 51 (2014).
M. Sathyadevi and S. Subramanian, Asian. J. Pharm. Clin. Res, l8, 152 (2015).
D. Thiyagarajan, S. Bharathi, A. Ayyaswamy and P. Raman, Int. J. Pharm. Pharm. Sci., 8, 120 (2016).
K.R. Markham, Techniques of Flavonoid Identification, London: Michigan: Academic Press (1982).
B.A. Chindo, J.A. Anuka, L. Mcneil, A.H. Yaro, S.S. Adamu, S. Amos, W.K. Connelly, G. Lees and K.S. Gamaniel, Brain Res. Bull., 78, 276 (2009); https://doi.org/10.1016/j.brainresbull.2008.12.005
W. Xu, K. Chu, H. Li, Y. Zhang, H. Zheng, R. Chen and L. Chen, Molecules, 17, 14323 (2012); https://doi.org/10.3390/molecules171214323

References

S. Sen, R. Chakraborty and B. De, J. Herbal Med., 1, 67 (2011); https://doi.org/10.1016/j.hermed.2011.11.001

, S. Saggar, P.A. Mir, N. Kumar, A. Chawla, J. Uppal, Shilpa and A. Kaur, Pharmacogn. Res., 14, 107 (2022); https://doi.org/10.5530/pres.14.2.15

R.P. Samy, P. Gopalakrishnakone, P. Houghton and S. Ignacimuthu, J. Ethnopharmacol., 107, 99 (2006); https://doi.org/10.1016/j.jep.2006.02.020

M.S. Pallie, P.K. Perera, N. Kumarasinghe, M. Arawwawala and C.L. Goonasekara, Evid. Based Complement Alternat Med., 2020, 8848676 (2020); https://doi.org/10.1155/2020/8848676

B. Saraceno, G. Avanzini and P. Lee, ‘Atlas: Epilepsy care in the world’, WHO, (2005).

C.R. Newton and H.H. Garcia, Lancet, 380, 1193 (2012); https://doi.org/10.1016/S0140-6736(12)61381-6

B.S. Reddy, N.R. Rao, K. Vijeepallam and V. Pandy, Afr. J. Tradit. Complement Altern. Med., 14, 105 (2017); https://doi.org/10.21010/ajtcam.v14i3.11

S. Vigneshwaran, K. Maharani, P. Sivasakthi, P.S. Selvan, S.D. Saraswathy and E.S. Priya, Inflammopharmacology, 31, 967 (2023); https://doi.org/10.1007/s10787-023-01154-8

G.A. Ayoola, O.O. Johnson, J. Aderounmu, L. Raji, R.B. Aremu, S. Bankole, Trop. J. Phytochem. Pharm. Sci., 3, 448 (2024); https://doi.org/10.26538/tjpps/v3i9.4

J.B. Harbone, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, New York: Chapman and Hall Publishers, pp. 49–188 (1973).

M.K. Shelar, M.J. Patil, S. Bhujbal and R.B. Chaudhari, Future J. Pharm. Sci., 4, 215 (2018); https://doi.org/10.1016/j.fjps.2018.06.002

H.O. Edeoga, D.E. Okwu and B.O. Mbaebie, Afr. J. Biotechnol., 4, 685 (2005); https://doi.org/10.5897/AJB2005.000-3127

E. Bienvenu, G.J. Amabeoku, P.K. Eagles, G. Scott and E.P. Springfield, Phytomedicine, 9, 217 (2002); https://doi.org/10.1078/0944-7113-00103

S. Patel, B.S. Meldrum and A. Fine, Behav. Brain Res., 31, 165 (1988); https://doi.org/10.1016/0166-4328(88)90019-8

V. Velu, S. Banerjee, V. Radhakrishnan, G. Gupta, D.K. Chellappan, N.K. Fuloria, S. Fuloria, M. Mehta, K. Dua and H. Malipeddi, Antiinflamm. Antiallergy Agents Med. Chem., 20, 308 (2021); https://doi.org/10.2174/1871523020666210126144506

M. Menon and L. Varghese, J. Appl. Biol. Biotechnol., 11, 84 (2023); https://doi.org/10.7324/JABB.2023.87672

P. Singh, I. Singh and R.K. Goel, Int. J. Pharm. Pharm. Sci., 6, 51 (2014).

M. Sathyadevi and S. Subramanian, Asian. J. Pharm. Clin. Res, l8, 152 (2015).

D. Thiyagarajan, S. Bharathi, A. Ayyaswamy and P. Raman, Int. J. Pharm. Pharm. Sci., 8, 120 (2016).

K.R. Markham, Techniques of Flavonoid Identification, London: Michigan: Academic Press (1982).

B.A. Chindo, J.A. Anuka, L. Mcneil, A.H. Yaro, S.S. Adamu, S. Amos, W.K. Connelly, G. Lees and K.S. Gamaniel, Brain Res. Bull., 78, 276 (2009); https://doi.org/10.1016/j.brainresbull.2008.12.005

W. Xu, K. Chu, H. Li, Y. Zhang, H. Zheng, R. Chen and L. Chen, Molecules, 17, 14323 (2012); https://doi.org/10.3390/molecules171214323

Author biographies is not available.