Novel 5-((Phenylimino)methyl)-1,2,4-triazol-3-one Derivatives: Synthesis, Anticancer Potential and Molecular Docking Insights

Rajitha Balavanthapu; Girija Sastry Vedula

doi:10.14233/ajchem.2025.33249

Issue

Vol. 37 No. 6 (2025): Vol 37 Issue 6, 2025

Issue Published : 27 May 2025

This work is licensed under a Creative Commons Attribution 4.0 International License.

Novel 5-((Phenylimino)methyl)-1,2,4-triazol-3-one Derivatives: Synthesis, Anticancer Potential and Molecular Docking Insights

https://doi.org/10.14233/ajchem.2025.33249

Rajitha Balavanthapu

Department of Chemistry, Andhra University, Visakhapatnam-530003, India

Girija Sastry Vedula

Department of Chemistry, Andhra University, Visakhapatnam-530003, India

Corresponding Author(s) : Rajitha Balavanthapu

rajitha.mpharm@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 6 (2025): Vol 37 Issue 6, 2025
Article Published : 27 May 2025

Abstract

In this study, a series of substituted 5-((phenylimino)methyl)-2,4-dihydro-3H-1,2,4-triazol-3-one derivatives (5a-o) were synthesized and evaluated for their anticancer activity against various human cancer cell lines, including A549 (non-small cell lung cancer), HCT-116 (colorectal cancer) and PANC-1 (pancreatic cancer). The compounds were synthesized through a multi-steps process involving the preparation of ethyl β-N-Boc-oxalamidrazone, followed by cyclization to form ethyl 5-oxo-4,5-dihydro-1H-1,2,4-triazole-3-carboxylate, reduction to yield 5-oxo-4,5-dihydro-1H-1,2,4-triazole-3-carbaldehyde and subsequent condensation with various substituted anilines to obtain the final derivatives. Structures of the compounds were confirmed by ¹H NMR, ¹³C NMR and high-resolution mass (HRMS) spectral methods. Molecular docking studies targeting epidermal growth factor receptor (EGFR) and cyclin-dependent kinase 4 (CDK4) revealed that certain derivatives, particularly those with electron-withdrawing groups like -NO₂, -OH and -CF₃, exhibited strong binding affinities, suggesting potential as inhibitors of these targets. In vitro cytotoxicity assays showed significant antiproliferative effects, with compound 5h (4-CF₃) exhibiting the highest potency against A549 (IC₅₀: 7.80 ± 3.06 µM) and PANC-1 (IC₅₀: 8.75 ± 1.86 µM). Compound 5j (3,4-(OCH₃)₂) demonstrated notable activity against A549 (IC₅₀: 5.96 ± 1.02 µM). Structure activity relationship analysis indicated that electron-donating groups generally enhanced activity, while electron-withdrawing groups had varying effects based on their position. These findings highlight the potential of these derivatives as lead compounds for anticancer drug development. Further optimization and in vivo studies are needed to fully explore their therapeutic potential.

Keywords

1,2,4-Triazoles Anticancer activity Molecular docking EGFR CDK

(1)

Balavanthapu, R.; Vedula, G. S. Novel 5-(Phenylimino)methyl)-1,2,4-Triazol-3-One Derivatives: Synthesis, Anticancer Potential and Molecular Docking Insights. ajc 2025, 37, 1257-1265.

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Author biographies is not available.