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Vol. 37 No. 5 (2025): Vol 37 Issue 5, 2025

Copyright (c) 2025 N.L. Hlabisa, A.K. Basson, Z.G. Ntombela, V.S.R.R. Pullabhotla

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Green Synthesis of Copper Nanoparticles using a Bioflocculant Extracted from Pseudomonas yamanorum and their Characterization

https://doi.org/10.14233/ajchem.2025.32807
N.L. Hlabisa
Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa
https://orcid.org/0000-0003-1208-1971
A.K. Basson
Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa
https://orcid.org/0000-0001-7685-2099
Z.G. Ntombela
Department of Biochemistry and Microbiology, Faculty of Science, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa
https://orcid.org/0000-0003-4107-2740
V.S.R.R. Pullabhotla
Department of Chemistry, Faculty of Science, Agriculture and Engineering, University of Zululand, P/Bag X1001, KwaDlangezwa 3886, South Africa
https://orcid.org/0000-0002-0093-460X

Corresponding Author(s) : V.S.R.R. Pullabhotla

pullabhotlav@unizulu.ac.za

Asian Journal of Chemistry, Vol. 37 No. 5 (2025): Vol 37 Issue 5, 2025

Abstract

This study used a bioflocculant produced by Pseudomonas yamanorum as a reducing and stabilizing agent in the green synthesis of copper nanoparticles (CuNPs). Several techniques like UV-Visible absorption, Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), thermogravimetric analysis (TGA) and transmission electron microscopy (TEM) were used to characterize the bioflocculant and biosynthesized CuNPs. Elemental analysis showed the existence of various elements, including copper (10.30 wt.%) in the CuNPs. FT-IR analysis showed carboxyl, hydroxyl and amide groups being present in the samples. The samples appeared almost spherical and agglomerated through SEM and TEM analysis, with sizes between 40 and 50 nm. The UV-visible spectra showed absorption peaks at 280 nm (bioflocculant) and 580 nm (CuNPs). After exposure to a high temperature of 900 ºC, the samples retained more than 60% of their weight, indicating good thermal stability.

Keywords

Bioflocculant Biosynthesis Copper nanoparticles Pseudomonas yamanorum
(1)
Hlabisa, N.; Basson, A.; Ntombela, Z.; Pullabhotla, V. Green Synthesis of Copper Nanoparticles Using a Bioflocculant Extracted from Pseudomonas Yamanorum and Their Characterization. ajc 2025, 37, 1127-1133.
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