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Vol. 37 No. 5 (2025): Vol 37 Issue 5, 2025

Copyright (c) 2025 Priya Agarwal, Satya Prakash, Gaurav Saini

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Efficacy of Protein Extracts of Moringa oleifera and Benincasa hispida Seeds for the Treatment of Microplastics

https://doi.org/10.14233/ajchem.2025.33515
Priya Agarwal
Department of Civil Engineering, Sharda University, Knowledge Park III, Greater Noida-201310, India
https://orcid.org/0000-0003-4252-8172
Satya Prakash
Department of Civil Engineering, Sharda University, Knowledge Park III, Greater Noida-201310, India
https://orcid.org/0000-0001-6633-7480
Gaurav Saini
Department of Civil Engineering, Netaji Subhas University of Technology, Sector-3, Dwarka, New Delhi-110078, India
https://orcid.org/0000-0002-3975-346X

Corresponding Author(s) : Priya Agarwal

priyamittal1199@gmail.com

Asian Journal of Chemistry, Vol. 37 No. 5 (2025): Vol 37 Issue 5, 2025

Abstract

Microplastics are emerging contaminants and have found significant interest due to their pervasive nature and potential health impacts on different life forms. A number of methods have been investigated so far, including the use of synthetic and recently natural coagulants, however, protein extracts derived from plant-based materials have not been extensively studied for such applications. Therefore, there is an urgent need to develop a sustainable treatment technology for the removal of microplastics. In this study, coagulation experiments were conducted under varying conditions such as pH, coagulant type and concentration using jar test apparatus for removal of microplastics from aqueous samples. The proteins extracted from two natural coagulants, Moringa oleifera and Benincasa hispida, have been studied for the removal of microplastics in aqueous samples. It was found that M. oleifera protein extracts achieved microplastics removal of 94.14 ± 11.0% at a dosage of 30 mL/L whereas B. hispida protein extracts achieved 88.29 ± 10.7% removal at 40 mL/L at pH 7. Therefore, the novelty of the study lies in evaluating the impact of M. oleifera and B. hispida seed protein extracts for the removal of microplastics. The findings suggest that the M. oleifera and B. hispida can be effective, sustainable, eco-friendly and affordable alternatives to chemical coagulants for treatment of microplastics in aqueous systems.

Keywords

Protein extracts Natural coagulants Sustainable Coagulation Microplastics
(1)
Agarwal, P.; Prakash, S.; Saini, G. Efficacy of Protein Extracts of Moringa Oleifera and Benincasa Hispida Seeds for the Treatment of Microplastics. ajc 2025, 37, 1118-1126.
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References
  1. D. Pan, F. Su, C. Liu and Z. Guo, Adv. Compos. Hybrid Mater., 3, 443 (2020); https://doi.org/10.1007/s42114-020-00190-0
  2. O. Bajt, FEBS Open Bio, 11, 954 (2021); https://doi.org/10.1002/2211-5463.13120
  3. Statista 2023, Annual Production of Plastics Worldwide from 1950 to 2023. Statista Research Department, Accessed on November 21, 2024; https://www.statista.com/statistics/282732/global-production-of-plastics-since-1950/
  4. A. Rafey and F.Z. Siddiqui, Int. J. Environ. Anal. Chem., 103, 3971 (2023); https://doi.org/10.1080/03067319.2021.1917560
  5. R. Geyer, J.R. Jambeck and K.L. Law, Sci. Adv., 3, e1700782 (2017); https://doi.org/10.1126/sciadv.1700782
  6. D.K.A. Barnes, F. Galgani, R.C. Thompson and M. Barlaz, Philos. Trans. R. Soc. Lond. B Biol. Sci., 364, 1985 (2009); https://doi.org/10.1098/rstb.2008.0205
  7. S. D’Angelo and R. Meccariello, Int. J. Environ. Res. Public Health, 18, 2392 (2021); https://doi.org/10.3390/ijerph18052392
  8. Z. Gao, K. Wontor, J.V. Cizdziel and H. Lu, Microplast. Nanoplast., 2, 10 (2022); https://doi.org/10.1186/s43591-022-00029-z
  9. M. Kushwaha, S. Shankar, D. Goel, S. Singh, J. Rahul, K. Rachna and J. Singh, Mar. Pollut. Bull., 209, 117109 (2024); https://doi.org/10.1016/j.marpolbul.2024.117109
  10. N. Bikiaris, N.F. Nikolaidis and P. Barmpalexis, Cosmetics, 11, 145 (2024); https://doi.org/10.3390/cosmetics11050145
  11. B. Udovicki, M. Andjelkovic, T. Cirkovic-Velickovic and A. Rajkovic, Int. J. Food Contam., 9, 7 (2022); https://doi.org/10.1186/s40550-022-00093-6
  12. R.U. Duru, E.E. Ikpeama and J.A. Ibekwe, Waste Dispos. Sustain. Energy, 1, 117 (2019); https://doi.org/10.1007/s42768-019-00010-2
  13. G. Suzuki, N. Uchida, K. Tanaka, O. Higashi, Y. Takahashi, H. Kuramochi, N. Yamaguchi and M. Osako, Environ. Pollut., 348, 123855 (2024); https://doi.org/10.1016/j.envpol.2024.123855
  14. A. Vega-Herrera, X. Borrell-Diaz, P.E. Redondo-Hasselerharm, E. Abad, M. Llorca, C.M. Villanueva and M. Farré, Water Res., 220, 118645 (2022); https://doi.org/10.1016/j.watres.2022.118645
  15. M. Kedzierski, B. Lechat, O. Sire, G. Le Maguer, V. Le Tilly and S. Bruzaud, Food Packag. Shelf Life, 24, 100489 (2020); https://doi.org/10.1016/j.fpsl.2020.100489
  16. L. Alberghini, A. Truant, S. Santonicola, G. Colavita and V. Giaccone, Int. J. Environ. Res. Public Health, 20, 789 (2022); https://doi.org/10.3390/ijerph20010789
  17. D.B. Daniel, P.M. Ashraf, S.N. Thomas and K.T. Thomson, Chemosphere, 264, 128554 (2021); https://doi.org/10.1016/j.chemosphere.2020.128554
  18. B. Basaran, Z. Özçifçi, E.D. Kanbur, H.T. Akçay, S. Gül, Y. Bektas and Ü. Aytan, J. Food Compos. Anal., 135, 106646 (2024); https://doi.org/10.1016/j.jfca.2024.106646
  19. P. Makhdoumi, M. Pirsaheb, A.A. Amin, S. Kianpour and H. Hossini, J. Food Compos. Anal., 119, 105261 (2023); https://doi.org/10.1016/j.jfca.2023.105261
  20. N. Manimozhi, V. Rani, C. Sudhan, D. Manimekalai, R. Shalini and K.M. Abarna, Reg. Stud. Mar. Sci., 53, 102350 (2022); https://doi.org/10.1016/j.rsma.2022.102350
  21. G. Kutralam-Muniasamy, F. Pérez-Guevara, I. Elizalde-Martínez and V.C. Shruti, Sci. Total Environ., 714, 136823 (2020); https://doi.org/10.1016/j.scitotenv.2020.136823
  22. D. Xing, T. Zhao, X. Tan, J. Liu, S. Wu, J. Xu, M. Yan, B. Sun, S. Liu and P. Zheng, Food Chem., 455, 139636 (2024); https://doi.org/10.1016/j.foodchem.2024.139636
  23. Y. Li, L. Peng, J. Fu, X. Dai and G. Wang, Analyst, 147, 1099 (2022); https://doi.org/10.1039/D2AN00083K
  24. N.-N. Lazãr, M. Cãlmuc, S.-A. Milea, P.-L. Georgescu and C. Iticescu, Heliyon, 10, e28291 (2024); https://doi.org/10.1016/j.heliyon.2024.e28291
  25. A. Saraluck, T. Techarang, P. Bunyapipat, K. Boonchuwong, Y. Pullaput and A. Mordmuang, J. Clin. Med., 13, 4029 (2024); https://doi.org/10.3390/jcm13144029
  26. E. Persiani, A. Cecchettini, E. Ceccherini, I. Gisone, M.A. Morales and F. Vozzi, Biomedicines, 11, 264 (2023); https://doi.org/10.3390/biomedicines11020264
  27. S.A. Arat, Water Pollut. Res., 89, 1771 (2024); https://doi.org/10.2166/wst.2024.082
  28. M.N. Issac and B. Kandasubramanian, Environ. Sci. Pollut. Res. Int., 28, 19544 (2021); https://doi.org/10.1007/s11356-021-13184-2
  29. K. Ziani, C.-B. Ionita-Mîndrican, M. Mititelu, S.M. Neacsu, C. Negrei, E. Morosan, D. Drãgãnescu and O.-T. Preda, Nutrients, 15, 617 (2023); https://doi.org/10.3390/nu15030617
  30. A. Nawab, M. Ahmad, M.T. Khan, M. Nafees, I. Khan and I. Ihsanullah, J. Hazard. Mater. Adv., 16, 100487 (2024); https://doi.org/10.1016/j.hazadv.2024.100487
  31. Y. Chen, Y. Meng, G. Liu, X. Huang, G. Chai and Y. Xie, Environ. Pollut., 355, 124192 (2024); https://doi.org/10.1016/j.envpol.2024.124192
  32. Y.S. Ibrahim, S. Tuan Anuar, A.A. Azmi, W.M.A. Wan Mohd Khalik, S. Lehata, S.R. Hamzah, D. Ismail, Z.F. Ma, A. Dzulkarnaen, Z. Zakaria, N. Mustaffa, S.E. Tuan Sharif and Y.Y. Lee, JGH Open, 5, 116 (2021); https://doi.org/10.1002/jgh3.12457
  33. M. Liu, J. Liu, F. Xiong, K. Xu, Y. Pu, J. Huang, J. Zhang, Y. Pu, R. Sun and K. Cheng, Environ. Geochem. Health, 45, 2803 (2023); https://doi.org/10.1007/s10653-022-01458-8
  34. K. Kadac-Czapska, J. Oœko, E. Knez and M. Grembecka, Antioxidants, 13, 579 (2024); https://doi.org/10.3390/antiox13050579
  35. W. Gao, Y. Zhang, A. Mo, J. Jiang, Y. Liang, X. Cao and D. He, Green Anal. Chem., 3, 100042 (2022); https://doi.org/10.1016/j.greeac.2022.100042
  36. L. Dayal, K. Yadav, U. Dey, K. Das, P. Kumari, D. Raj and R.R. Mandal, JJ. Hazard. Mater. Adv., 16, 100460 (2024); https://doi.org/10.1016/j.hazadv.2024.100460
  37. S. Ziajahromi, P.A. Neale, L. Rintoul and F.D.L. Leusch, Water Res., 112, 93 (2017); https://doi.org/10.1016/j.watres.2017.01.042
  38. N. Girish, N. Parashar and S. Hait, Sci. Total Environ., 899, 165723 (2023); https://doi.org/10.1016/j.scitotenv.2023.165723
  39. B. Koul, N. Bhat, M. Abubakar, M. Mishra, A.P. Arukha and D. Yadav, Water, 14, 3751 (2022); https://doi.org/10.3390/w14223751
  40. P. Agarwal, S. Prakash and G. Saini, Cleaner Water, 1, 100010 (2024); https://doi.org/10.1016/j.clwat.2024.100010
  41. T. Reza, Z.H. Mohamad Riza, S.R. Sheikh Abdullah, H. Abu Hasan, N. Ismail and A.R. Othman, Toxics, 12, 12 (2023); https://doi.org/10.3390/toxics12010012
  42. S. Mathupreetha, W.M.A.P. Wijekoon, W.K.C.N. Dayanthi and L.F.U. Nusrath, Total Environ. Eng., 2, 100009 (2025); https://doi.org/10.1016/j.teengi.2025.100009
  43. M.H. Abdul-Rahman-Adrin, N. Ngadiman, N.H. Abdullah, R. Hamdan and M.S. Abdullah, IOP Conf. Ser. Earth Environ. Sci., 1453, 012052 (2025); https://doi.org/10.1088/1755-1315/1453/1/012052
  44. C. Panigrahi, S. Kamal, J. Qin, S. Ziemann, E. Rahman, M. House, C. Dutcher and B. Xiong, Environ. Eng. Sci., 41, 477 (2024); https://doi.org/10.1089/ees.2024.0135
  45. E. Ali, S. Muyibi, H. Salleh, M. Alam and M. Salleh, J. Water Resour. Prot., 2, 259 (2010); https://doi.org/10.4236/jwarp.2010.23030
  46. S.A. Muyibi, S.A. Abbas, M.J.M.M. Noor and F.R. Ahmadon, IIUM Eng. J., 4, 1 (2003).
  47. S.B. Kurniawan, A. Ahmad, N.S.M. Said, K. Gustinasari, S.R.S. Abdullah and M.F. Imron, Environ. Adv., 14, 100441 (2023); https://doi.org/10.1016/j.envadv.2023.100441
  48. M. Bradford, Anal. Biochem., 72, 248 (1976); https://doi.org/10.1016/0003-2697(76)90527-3
  49. D. Churiyah and L.K. Darusman, Hayati J. Biosci., 16, 161 (2009); https://doi.org/10.4308/hjb.16.4.161
  50. R. Chércoles Asensio, M. San Andrés Moya, J.M. de la Roja and M. Gómez, Anal. Bioanal. Chem., 395, 2081 (2009); https://doi.org/10.1007/s00216-009-3201-2
  51. S. Avazpour and M. Noshadi, Chemosphere, 358, 142215 (2024); https://doi.org/10.1016/j.chemosphere.2024.142215
  52. S. Prasetyo, C.A. Santos, A.K. Sugih and H. Kristianto, Sustain. Chem. Environ., 9, 100225 (2025); https://doi.org/10.1016/j.scenv.2025.100225
  53. J. Yao, Z. Peng, W. Chen, Q. Lin, M. Cheng, H. Li, Y. Yang and H.Y. Yang, Mar. Pollut. Bull., 186, 114347 (2023); https://doi.org/10.1016/j.marpolbul.2022.114347
  54. C. Li, R. Busquets and L.C. Campos, Chemosphere, 364, 143145 (2024); https://doi.org/10.1016/j.chemosphere.2024.143145
  55. K. Yu, Y. Zhang, S. Yang and L. Chen, Environ. Eng. Sci., 41, 459 (2024); https://doi.org/10.1089/ees.2023.0332
  56. S. Ziembowicz, M. Kida and P. Koszelnik, Materials, 16, 4070 (2023); https://doi.org/10.3390/ma16114070
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