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Vol. 37 No. 2 (2025): Vol 37 Issue 2, 2025

Copyright (c) 2025 V Ravichandra Reddy V, Proff. P. Radhika; Professor & BOS, Chairman, Dr. Chidananda Swamy

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Isolation and Characterization of Anticancer Properties of Compounds from the Leaf Extracts of Vitex negundo

https://doi.org/10.14233/ajchem.2025.33147
V.V. Ravichandra Reddy
Department of Medicinal Chemistry (Analytical R&D), Aragen Life Sciences Private Limited, IDA, Mallapur, Hyderabad-500076, India; Department of Biochemistry, Andhra University, Visakhapatnam-530003, India
https://orcid.org/0000-0002-8490-0166
P. Radhika
Department of Biochemistry, Andhra University, Visakhapatnam-530003, India
https://orcid.org/0000-0002-4596-0182
R. Chidananda Swamy
Department of Medicinal Chemistry (Analytical R&D), Aragen Life Sciences Private Limited, IDA, Mallapur, Hyderabad-500076, India
https://orcid.org/0000-0002-8437-8347
D. Raju
Department of Medicinal Chemistry (Analytical R&D), Aragen Life Sciences Private Limited, IDA, Mallapur, Hyderabad-500076, India
https://orcid.org/0000-0002-5706-0691

Corresponding Author(s) : P. Radhika

parvataneni_radhika@yahoo.com

Asian Journal of Chemistry, Vol. 37 No. 2 (2025): Vol 37 Issue 2, 2025

Abstract

Vitex negundo belongs to the family of Lamiaceae and is popularly known as sambhaloo or Chinese chaste tree. It is widely used as Indian medicinal shrub to prevent a variety of ailments including cancer. The aim of the study was to investigate the anticancer activity of solvent extracts and fractionates of Vitex negundo (VN) leaves against human lung carcinoma epithelial cells A549 by MTT assay. The leaves were extracted with various solvents by ultrasonic-assisted extraction method, in the sequel of non-polar to polar solvents from n-hexane, chloroform, ethyl acetate and ethanol:water (1:1). Among the four solvent extracts, the ethanol:water (1:1) extract has shown the significant anticancer activity (IC50 = 44.31 ± 0.61 at 5 to 300 µg/mL). Four components were isolated from ethanol:water (1:1) extract. The structure of these compounds were elucidated and identified as 4-OH benzoic acid (VN-EtOH+H2O-P1), negundoside (VN-EtOH+H2O-P2), isoorientin (VN-EtOH+H2O-P3) and agnuside (VN-EtOH+H2O-P4) by NMR, LCMS, FTIR and melting point. Among the four fractionated products, isoorientin showed the significant cytotoxic activity against A549 cells with IC50 value 18.50 ± 0.76 µM/mL. The standard drug, cisplatin IC50 values for anticancer activity of crude extracts (IC50 value 13.45 ± 0.084 µM/mL) were compared with that of pure compounds (IC50 value 14.27 ± 0.143 µM/mL). The bioactive compound, isoorientin (VN-EtOH+H2O-P3) as well as the standard drug, doxorubicin treated with normal human dermal fibroblast (HDF) cells after 48 h, with an IC50 values of 416.57 ± 0.01µM/mL and 19.8 ± 0.01 µM/mL).

Keywords

Anticancer activity Vitex negundo MTT assay Cytotoxicity PREP-HPLC
Reddy, V. R., Radhika, P., Swamy, R. C., & Raju, D. (2025). Isolation and Characterization of Anticancer Properties of Compounds from the Leaf Extracts of Vitex negundo. Asian Journal of Chemistry, 37(2), 439–446. https://doi.org/10.14233/ajchem.2025.33147
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References
  1. I. Bourais, S. Elmarrkechy, D. Taha, Y. Mourabit, A. Bouyahya, M. El Yadini, O. Machich, S. El Hajjaji, H. El Boury, N. Dakka and N. Iba, Food Rev. Int., 39, 6199 (2022); https://doi.org/10.1080/87559129.2022.2094401
  2. H.S.R. Rajula, G. Verlato, M. Manchia, N. Antonucci and V. Fanos, Medicina (B. Aires), 56, 455 (2020); https://doi.org/10.3390/medicina56090455
  3. C. Claus, C. Ferrara-Koller and C. Klein, MAbs, 15, 2167189 (2023); https://doi.org/10.1080/19420862.2023.2167189
  4. K. Gouthami and L. Lavanya, Indian J. Biochem. Biophys., 61, (2024); https://doi.org/10.56042/ijbb.v61i5.2015
  5. S.M. Hawkins and C.D. Robacker, J. Environ. Hortic., 37, 24 (2019); https://doi.org/10.24266/0738-2898-37.1.24
  6. N. Kamal, N.S. Mio Asni, I.N.A. Rozlan, M.A.H. Mohd Azmi, N.W. Mazlan, A. Mediani, S.N. Baharum, J. Latip, S. Assaw and R.A. Edrada-Ebel, Plants, 11, 1944 (2022); https://doi.org/10.3390/plants11151944
  7. M.F. Khan, P. Arora and M. Dhobi, Curr. Tradit. Med., 7, 138 (2021); https://doi.org/10.2174/2215083805666191021161005
  8. L.S.S. Mesquita, T.R.S.A. Luz, J.W.C. Mesquita, D.F. Coutinho, F.M.M. Amaral, M.N.S. Ribeiro and S. Malik, Food Rev. Int., 35, 105 (2018); https://doi.org/10.1080/87559129.2018.1467443
  9. P.S. Palaninathan, A.S. Kamalabai Raveendran and J. Swaminathan Kesavan, Int. J. Nutr. Pharmacol. Neurol. Dis., 12, 319 (2022); https://doi.org/10.4103/ijnpnd.ijnpnd_77_22
  10. K. Chaitanya Thandra, A. Barsouk, K. Saginala, J. Sukumar Aluru and A. Barsouk, K.C. Thandra, A. Barsouk, K. Saginala, J.S. Aluru and A. Barsouk, Contemp. Oncol., 25, 45 (2021); https://doi.org/10.5114/wo.2021.103829
  11. P. Panda, B. Das, D.S. Sahu, S.K. Meher, B.K. Das and G.C. Nanda, Res. J. Pharmacol. Pharmacodyn., 6, 162 (2014).
  12. K.V. Sonawala, P. Bhat and K.M. Sweta, J. Ayurv. Integr. Med. Sci., 9, 283 (2024); https://doi.org/10.21760/jaims.9.4.46
  13. S.S. Patil, A. Pathak and V.K. Rathod, Ultrason. Sonochem., 70, 105267 (2021); https://doi.org/10.1016/j.ultsonch.2020.105267
  14. B. Lin, S. Wang, A. Zhou, Q. Hu and G. Huang, Ultrason. Sonochem., 98, 106507 (2023); https://doi.org/10.1016/j.ultsonch.2023.106507
  15. A. Chapagain, D. Acharya, A.K. Das, K. Chhetri, H.B. Oli and A.P. Yadav, Electrochem, 3, 211 (2022); https://doi.org/10.3390/electrochem3020013
  16. X. Lu, N. Li, R. Zhao, M. Zhao, X. Cui, Y. Xu and X. Qiao, Front. Nutr., 8, 798450 (2021); https://doi.org/10.3389/fnut.2021.798450
  17. S. Omonmhenle and O. Iyekowa, NIPES-J. Sci. Technol. Res., 5, 40 (2023); https://doi.org/10.5281/zenodo.8010016
  18. A. Abdelraheem, R. Tukra, P. Kazarin, M.D. Sinanis, E.M. Topp, A. Alexeenko and D. Peroulis, PNAS Nexus, 1, 1 (2022); https://doi.org/10.1093/pnasnexus/pgac052
  19. E. Vasconcelos Soares Maciel, A.L. de Toffoli, E. Sobieski, C.E. Domingues Nazário and F.M. Lanças, Anal. Chim. Acta, 11, 1103 (2020); https://doi.org/10.1016/j.aca.2019.12.064
  20. Y. Ben-Tal, P.J. Boaler, H.J.A. Dale, R.E. Dooley, N.A. Fohn, Y. Gao, A. García-Domínguez, K.M. Grant, A.M.R. Hall, H.L.D. Hayes, M.M. Kucharski, R. Wei and G.C. Lloyd-Jones, Prog. Nucl. Magn. Reson. Spectrosc., 129, 28 (2022); https://doi.org/10.1016/j.pnmrs.2022.01.001
  21. R. Hoffman, J. Magn. Reson., 335, 107105 (2022); https://doi.org/10.1016/j.jmr.2021.107105
  22. Ü. Babacan, A. Kaba, F. Akçakale, M.F. Cengiz and E. Akinci, Int. J. Life Sci. Biotechnol., 5, 9 (2022); https://doi.org/10.38001/ijlsb.991615
  23. N.T.H. Nga, T.T.B. Ngoc, N.T.M. Trinh, T.L. Thuoc and D.T.P. Thao, Anal. Biochem., 610, 113937 (2020); https://doi.org/10.1016/j.ab.2020.113937
  24. D. Grasso, L.X. Zampieri, T. Capelôa, J.A. Van de Velde and P. Sonveaux, Cell Stress, 4, 114 (2020); https://doi.org/10.15698/cst2020.06.221
  25. P.E. Porporato, N. Filigheddu, J.M. Pedro, G. Kroemer and L. Galluzzi, Cell Res., 28, 265 (2018); https://doi.org/10.1038/cr.2017.155
  26. P. Ghosh, C. Vidal, S. Dey and L. Zhang, Int. J. Mol. Sci., 21, 3363 (2020); https://doi.org/10.3390/ijms21093363
  27. M. Neagu, C. Constantin, I.D. Popescu, D. Zipeto, G. Tzanakakis, D. Nikitovic, C. Fenga, C.A. Stratakis, D.A. Spandidos and A.M. Tsatsakis, Front. Oncol., 9, 348 (2019); https://doi.org/10.3389/fonc.2019.00348
  28. R.C. Santhanam, S.A.M. Yacoob and A. Venkatraman, Braz. J. Pharm. Sci., 58, e19542 (2022); https://doi.org/10.1590/s2175-97902022e19542
  29. W.T. Xu, G.N. Shen, T.Z. Li, Y. Zhang, T. Zhang, H. Xue, W.B. Zuo, Y.N. Li, D.J. Zhang and C.H. Jin, Int. J. Oncol., 57, 550 (2020); https://doi.org/10.3892/ijo.2020.5079
  30. H.K. Huang, S.Y. Lee, S.F. Huang, Y.S. Lin, S.C. Chao, S.F. Huang, S.C. Lee, T.H. Cheng, S.H. Loh and Y.T. Tsai, Am. J. Chin. Med., 48, 201 (2020); https://doi.org/10.1142/S0192415X20500111
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